Skip to main content

Nd:YAG laser irradiation associated with fluoridated gels containing photo absorbers in the prevention of enamel erosion

Lasers in Medical Science volume 32, pages 1453–1459 (2017)Cite this article

An Erratum to this article was published on 20 June 2017

This article has been updated

Abstract

This study evaluated the combined effect of Nd:YAG laser irradiation and fluoridated gels containing photo absorbers against enamel erosion. Enamel specimens from bovine teeth were polished, eroded (10 min, with 1% citric acid, pH = 2.6), and randomly allocated into the experimental groups (n = 8), according to the different surface treatments: fluoridated gels (F: 9047 ppm F and F + Sn: 9047 ppm F and 3000 ppm Sn), with or without photo absorbers (E: erythrosine and MB: methylene blue), and associated or not with Nd:YAG laser irradiation (in contact; 0.5 W; 50 mJ; ~41.66 J/cm2; 10 Hz; 40 s; pulse duration of 120 μs). A placebo gel (PLA) associated or not with laser was used as control. All gels had pH = 4.5 and were applied for 2 min. Laser irradiation was performed during gel application. The specimens were then submitted to a 5-day erosion-remineralization cycling model using 0.3% citric acid (pH = 2.6), 4×/day. Enamel surface loss (SL) was analyzed by optical profilometry in the end of the cycling (in μm). Data were analyzed by ANOVA and Tukey tests (α = 0.05). Means (SD) of SL for the groups were the following (different superscript letters imply significant difference among groups): PLA (21.02 ± 1.28)a, PLA + laser (19.20 ± 0.96)ab, laser (17.47 ± 1.50)b, F + Sn + E + laser (13.69 ± 0.62)c, F + E + laser (13.52 ± 1.16)c, F (13.10 ± 1.08)c, F + laser (11.94 ± 1.44)cd, F + Sn + MB + laser (11.90 ± 4.02)cd, F + MB + laser (11.42 ± 1.42)cd, F + Sn (11.12 ± 1.20)cd, and F + Sn + laser (10.35 ± 0.89)d. In conclusion, all fluoridated gels and the Nd:YAG laser irradiation reduced erosion development, but the combination of treatments did not promote further protection. The addition of photo absorbers to the fluoridated gels did not influence the anti-erosive effect of the combination of laser plus fluoridated gels.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1

Change history

  • 20 June 2017

    An erratum to this article has been published.

References

  1. Lussi A, Carvalho TS (2014) Erosive tooth wear: a multifactorial condition of growing concern and increasing knowledge. Monogr Oral Sci 25:1–15. doi:10.1159/000360380

    Article  PubMed  Google Scholar 

  2. ten Cate JM, Imfeld T (1996) Dental erosion, summary. Eur J Oral Sci 104:241–244

    Article  PubMed  Google Scholar 

  3. Addy M, Shellis RP (2014) Interaction between attrition, abrasion and erosion in tooth wear. Monogr Oral Sci 20:17–31. doi:10.1159/000093348

    Article  Google Scholar 

  4. Lussi A, Schlueter N, Rakhmatullina E, Ganss C (2011) Dental erosion—an overview with emphasis on chemical and histopathological aspects. Caries Res 45:2–12. doi:10.1159/000325915

    Article  PubMed  Google Scholar 

  5. Schlueter N, Jaeggi T, Lussi A (2012) Is dental erosion really a problem? Adv Dent Res 24:68–71. doi:10.1177/0022034512449836

    Article  CAS  PubMed  Google Scholar 

  6. Jaeggi T, Lussi A (2014) Prevalence, incidence and distribution of erosion. Monogr Oral Sci 25:55–73. doi:10.1159/000360973

    Article  PubMed  Google Scholar 

  7. Scaramucci T, Borges AB, Lippert F et al (2013) Sodium fluoride effect on erosion-abrasion under hyposalivatory simulating conditions. Arch Oral Biol. doi:10.1016/j.archoralbio. 2013.06.004

    PubMed  Google Scholar 

  8. Huysmans M-C, Young A, Ganss C (2014) The role of fluoride in erosion therapy. Monogr Oral Sci 25:230–243. doi:10.1159/000360555

    Article  PubMed  Google Scholar 

  9. Scaramucci T, Borges AB, Lippert F et al (2015) Anti-erosive properties of solutions containing fluoride and different film-forming agents. J Dent 43:458–465. doi:10.1016/j.jdent. 2015.01.007

    Article  CAS  PubMed  Google Scholar 

  10. Hove LH, Holme B, Young A, Tveit AB (2008) The protective effect of tif 4, SnF2 and NaF against erosion-like lesions in situ. Caries Res 42:68–72. doi:10.1159/000112816

    Article  CAS  PubMed  Google Scholar 

  11. Ganss C, Klimek J, Schäffer U, Spall T Effectiveness of two fluoridation measures on erosion progression in human enamel and dentine in vitro. Caries Res 35:325–330 doi: 47470

  12. Venasakulchai A, Williams NA, Gracia LH, Rees GD (2010) A comparative evaluation of fluoridated and non-fluoridated mouthrinses using a 5-day cycling enamel erosion model. J Dent 38:S21–S29. doi:10.1016/S0300-5712(11)70005-7

    Article  CAS  PubMed  Google Scholar 

  13. Lagerweij MD, Buchalla W, Kohnke S et al (2006) Prevention of erosion and abrasion by a high fluoride concentration gel applied at high frequencies. Caries Res 40:148–153. doi:10.1159/000091062

    Article  CAS  PubMed  Google Scholar 

  14. Austin RS, Rodriguez JM, Dunne S et al (2010) The effect of increasing sodium fluoride concentrations on erosion and attrition of enamel and dentine in vitro. J Dent 38:782–787. doi:10.1016/j.jdent. 2010.06.009

    Article  CAS  PubMed  Google Scholar 

  15. João-Souza SH, Bezerra SJC, Borges AB et al (2015) Effect of sodium fluoride and stannous chloride associated with Nd:YAG laser irradiation on the progression of enamel erosion. Lasers Med Sci. doi:10.1007/s10103-015-1791-9

    Google Scholar 

  16. Magalhães AC, Rios D, Machado MADA et al (2008) Effect of Nd:YAG irradiation and fluoride application on dentine resistance to erosion in vitro. Photomed Laser Surg 26:559–563. doi:10.1089/pho. 2007.2231

    Article  PubMed  Google Scholar 

  17. Rios D, Magalhães AC, de AM MMA et al (2009) In vitro evaluation of enamel erosion after Nd:YAG laser irradiation and fluoride application. Photomed Laser Surg 27:743–747. doi:10.1089/pho. 2008.2384

    Article  PubMed  Google Scholar 

  18. Tagomori S, Iwase T (1995) Ultrastructural change of enamel exposed to a normal pulsed Nd-YAG laser. Caries Res 29:513–520

    Article  CAS  PubMed  Google Scholar 

  19. Castellan CS, Luiz AC, Bezinelli LM et al (2007) In vitro evaluation of enamel demineralization after Er:YAG and Nd:YAG laser irradiation on primary teeth. Photomed Laser Surg 25:85–90. doi:10.1089/pho. 2006.2043

    Article  CAS  PubMed  Google Scholar 

  20. Vlacic J, Meyers IA, Walsh LJ (2007) Laser-activated fluoride treatment of enamel as prevention against erosion. Aust Dent J 52:175–180. doi:10.1111/j.1834-7819. 2007.tb 00485.x

    Article  CAS  PubMed  Google Scholar 

  21. Gao X-LX-L, Pan J-SJ-SJ-S, Hsu C-YC-Y (2006) Laser-fluoride effect on root demineralization. J Dent Res 85:919–923. doi:10.1177/154405910608501009

    Article  PubMed  Google Scholar 

  22. Kwon YH, Kwon O-W, Kim H-I, Kim K-H (2003) Nd:YAG laser ablation and acid resistance of enamel. Dent Mater J 22:404–411

    Article  CAS  PubMed  Google Scholar 

  23. Zezell DM, Boari HGD, Ana PA et al (2009) Nd:YAG laser in caries prevention: a clinical trial. Lasers Surg Med 41:31–35. doi:10.1002/lsm.20738

    Article  PubMed  Google Scholar 

  24. Yonaga K, Kimura Y, Matsumoto K (1999) Treatment of cervical dentin hypersensitivity by various methods using pulsed Nd:YAG laser. J Clin Laser Med Surg 17:205–210. doi:10.1089/clm. 1999.17.205

    CAS  PubMed  Google Scholar 

  25. Zapletalova Z, Perina JJ, Novotny R, Chmelickova H (2007) Suitable conditions for sealing of open dentinal tubules using a pulsed Nd:YAG laser. Photomed Laser Surg 25:495–499. doi:10.1089/pho. 2007.2085

    Article  PubMed  Google Scholar 

  26. Pancote LP, Manarelli MM, Danelon M, Delbem ACB (2014) Effect of fluoride gels supplemented with sodium trimetaphosphate on enamel erosion and abrasion: in vitro study. Arch Oral Biol 59:336–340. doi:10.1016/j.archoralbio. 2013.12.007

    Article  CAS  PubMed  Google Scholar 

  27. Ganss C, Klimek J, Schlueter N (2014) Erosion/abrasion-preventing potential of NaF and F/Sn/chitosan toothpastes in dentine and impact of the organic matrix. Caries Res 48:163–169. doi:10.1159/000354679

    Article  CAS  PubMed  Google Scholar 

  28. Scaramucci T, João-Souza SH, Lippert F et al (2016) Influence of toothbrushing on the antierosive effect of film-forming agents. Caries Res 50:104–110. doi:10.1159/000443619

    Article  CAS  PubMed  Google Scholar 

  29. Scaramucci T, Borges AB, Lippert F et al (2013) Sodium fluoride effect on erosion-abrasion under hyposalivatory simulating conditions. Arch Oral Biol 58:1457–1463. doi:10.1016/j.archoralbio. 2013.06.004

    Article  CAS  PubMed  Google Scholar 

  30. Zhang C, Kimura Y, Matsumoto K et al (1998) Effects of pulsed Nd:YAG laser irradiation on root canal wall dentin with different laser initiators. J Endod 24:352–355. doi:10.1016/S0099-2399(98)80133-7

    Article  CAS  PubMed  Google Scholar 

  31. Maleki-Pour MR, Birang R, Khoshayand M, Naghsh N (2015) Effect of Nd:YAG laser irradiation on the number of open dentinal tubules and their diameter with and without smear of graphite: an in vitro study. J lasers Med Sci 6:32–39

    PubMed  PubMed Central  Google Scholar 

  32. Meurman JH, Hemmerlé J, Voegel JC et al (1997) Transformation of hydroxyapatite to fluorapatite by irradiation with high-energy CO2 laser. Caries Res 31:397–400

    Article  CAS  PubMed  Google Scholar 

  33. Magalhães AC, Romanelli AC, Rios D et al (2011) Effect of a single application of TiF4 and NaF varnishes and solutions combined with Nd:YAG laser irradiation on enamel erosion in vitro. Photomed Laser Surg 29:537–544. doi:10.1089/pho. 2010.2886

    Article  PubMed  Google Scholar 

  34. Braga SRM, de Oliveira E, Sobral MAP (2015) Effect of neodymium: yttrium-aluminum-garnet laser and fluoride on the acid demineralization of enamel. J Investig Clin Dent. doi:10.1111/jicd.12185

    PubMed  Google Scholar 

  35. Bedini R, Manzon L, Fratto G, Pecci R (2010) Microhardness and morphological changes induced by Nd:Yag laser on dental enamel: an in vitro study. Ann dell’Istituto Super di sanità 46:168–172. doi:10.4415/ANN_10_02_10

    Google Scholar 

  36. Majori M, Manzon L, Pane S, Bedini R Effects of Nd:YAG laser on dental enamel. J Appl Biomater Biomech 3:128–133

  37. Liu Y, Hsu C-YSC-YS, Teo CMJ, Teoh SH (2013) Potential mechanism for the laser-fluoride effect on enamel demineralization. J Dent Res 92:71–75. doi:10.1177/0022034512466412

    Article  CAS  PubMed  Google Scholar 

  38. Fowler BO, Kuroda S (1986) Changes in heated and in laser-irradiated human tooth enamel and their probable effects on solubility. Calcif Tissue Int 38:197–208

    Article  CAS  PubMed  Google Scholar 

  39. Hsu C-YS, Jordan TH, Dederich DN, Wefel JS (2001) Laser-matrix-fluoride effects on enamel demineralization. J Dent Res 80:1797–1801. doi:10.1177/00220345010800090501

    Article  CAS  PubMed  Google Scholar 

  40. Nelson DGA, Wefel JS, Jongebloed WL, Featherstone JDB (1987) Morphology, histology and crystallography of human dental enamel treated with pulsed low-energy infrared laser radiation. Caries Res 21:411–426. doi:10.1159/000261047

    Article  CAS  PubMed  Google Scholar 

  41. Magalhães AC, Wiegand A, Rios D et al (2011) Fluoride in dental erosion. Fluoride Oral Environ 22:158–170. doi:10.1159/000325167

    Article  Google Scholar 

  42. Ganss C, Schlueter N, Klimek J (2007) Retention of KOH-soluble fluoride on enamel and dentine under erosive conditions—a comparison of in vitro and in situ results. Arch Oral Biol 52:9–14. doi:10.1016/j.archoralbio. 2006.07.004

    Article  CAS  PubMed  Google Scholar 

  43. Kadajji VG, Betageri GV (2011) Water soluble polymers for pharmaceutical applications. Polymers (Basel) 3:1972–2009. doi:10.3390/polym 3041972

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank FAPESP (São Paulo Research Foundation, Grant Nos. 2012/20632-9, 2011/17699-1, and 2014/14055-4), CNPQ (National Council of Technological and Scientific Development, for the scholarship of the first author), and LELO (Special Laboratory of Lasers in Dentistry from the Department of Restorative Dentistry at the School of Dentistry of the University of São Paulo, Brazil).

Author information

Authors and Affiliations

  1. Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes 2227, São Paulo, SP, 05508-000, Brazil

    LGS Pereira, SH Joao-Souza, SJC Bezerra, ACC Aranha & T Scaramucci

  2. Department of Restorative Dentistry, Institute of Science and Technology, São Paulo State University-UNESP, Av. Eng. Francisco Jose Longo 777, Jardim São Dimas, Sao Jose dos Campos, SP, 12245-000, Brazil

    AB Borges

Authors
  1. LGS Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. SH Joao-Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. SJC Bezerra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. AB Borges
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. ACC Aranha
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T Scaramucci
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T Scaramucci.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

For this type of study, formal consent is not required.

Funding

The work was supported by the São Paulo Research Foundation (FAPESP, Grant No. 2014/14055-4) and the National Council of Technological and Scientific Development (CNPQ, for the scholarship of the first author).

Additional information

The original version of this article was revised: The first author name was incorrectly captured. The given name and family name were interchanged. The correct name is shown in the author group section.

An erratum to this article is available at https://doi.org/10.1007/s10103-017-2248-0.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Pereira, L., Joao-Souza, S., Bezerra, S. et al. Nd:YAG laser irradiation associated with fluoridated gels containing photo absorbers in the prevention of enamel erosion. Lasers Med Sci 32, 1453–1459 (2017). https://doi.org/10.1007/s10103-017-2226-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-017-2226-6

Keywords

  • Tooth erosion
  • Sodium fluoride
  • Stannous chloride
  • Nd:YAG laser
  • Optical profilometry