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Evaluation of the effectiveness of micro-Raman spectroscopy in monitoring the mineral contents change of human enamel in vitro

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Abstract

The purpose of this in vitro study was to investigate the efficacy of micro-Raman spectroscopy on detecting mineral content change during the demineralization and de/remineralization cycling process. The enamel samples (n = 55) were randomly divided into three groups and separately treated with demineralization solution (n = 20), de/remineralization cycling solution (n = 30), and distilled water (n = 5). Micro-Raman spectroscopy, microhardness (MHS), and the released calcium ions concentration were performed before and after treatment, respectively. A one-way analysis of variance (ANOVA) with a post hoc Tukey test was used to analyze the results. The Spearman correlation coefficients among the parameters of Raman relative intensity decrease (RRID%), the percentage of MHS loss (PML), and the released calcium ions concentration were also analyzed. In demineralization group, RRID%, PML, and released calcium ions concentration were highly correlated with each other (r = 0.979, p < 0.001; r = 0.984, p < 0.001; and r = 0.983, p < 0.001, respectively). While for the de/remineralization cycling group, there also existed a high correlation between RRID% and PML (r = 0.987, p < 0.001). In conclusion, micro-Raman spectroscopy could effectively monitor the mineral content change, and its efficacy was validated by the measurement of released calcium ions concentration and MHS.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 81500887 and 81071190).

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Author notes

  1. Yue Sa and Xiaowei Feng contributed equally to this work.

Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People’s Republic of China

    Yue Sa, Xiaowei Feng, Chang Lei, Yan Yu, Tao Jiang & Yining Wang

  2. Department of Prosthodontics, Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, People’s Republic of China

    Yue Sa, Tao Jiang & Yining Wang

  3. Tianjin Stomatological Hospital, Nankai University, 75 Dagu Road, Heping District, Tianjin, 300000, People’s Republic of China

    Yan Yu

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  1. Yue Sa
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  2. Xiaowei Feng
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  3. Chang Lei
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  4. Yan Yu
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Correspondence to Tao Jiang or Yining Wang.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments and was approved by the Ethics Committee of the School and Hospital of Stomatology, Wuhan University.

Informed consent

Informed consent was obtained from all individual participants whose extracted premolars were used in the study.

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Sa, Y., Feng, X., Lei, C. et al. Evaluation of the effectiveness of micro-Raman spectroscopy in monitoring the mineral contents change of human enamel in vitro. Lasers Med Sci 32, 985–991 (2017). https://doi.org/10.1007/s10103-017-2197-7

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  • DOI: https://doi.org/10.1007/s10103-017-2197-7

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