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Acute LED irradiation does not change the anaerobic capacity and time to exhaustion during a high-intensity running effort: a double-blind, crossover, and placebo-controlled study

Effects of LED irradiation on anaerobic capacity and performance in running

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The purpose of this study was to investigate the acute effects of photobiomodulation therapy using cluster light-emitting diodes (LEDT; 104 diodes) (wavelength 660 and 850 nm; energy density 1.5 and 4.5 J/cm2; energy 60 J at each point; total energy delivered 600 J) on alternative maximal accumulated oxygen deficit (MAODALT) and time to exhaustion, during a high-intensity running effort. Fifteen moderately active and healthy males (age 25.1 ± 4.4 years) underwent a graded exercise test and two supramaximal exhaustive efforts at 115 % of the intensity associated with maximal oxygen uptake performed after acute LEDT or placebo irradiation in a double-blind, crossover, and placebo-controlled study design. The MAODALT was assumed as the sum of both oxygen equivalents estimated from the glycolytic and phosphagen metabolism pathways during each supramaximal effort. For the statistical analysis, a paired t test was used to determine differences between the treatments. The significance level was assumed as 95 %. In addition, a qualitative analysis was used to determine the magnitude of differences between groups. No significant differences were found for the values of oxygen equivalents from each energetic metabolism (P ≥ 0.28), for MAODALT values between the LEDT and placebo conditions (P ≥ 0.27), or for time to exhaustion (P = 0.80), except for the respiratory exchange ratio (P = 0.01). The magnitude-based inference of effect size reported only a possibly negative effect of photobiomodulation on MAODALT when expressed in units relative to body mass and on the glycolysis pathway (26 %). In summary, LEDT after a high-intensity running effort did not alter the MAODALT, metabolic energy pathways, or high-intensity running performance.

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  1. Anders JJ, Lanzafame RJ, Arany PR (2015) Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg 33:183–184

    Article  PubMed  PubMed Central  Google Scholar 

  2. Ferraresi C, Kaippert B, Avci P, Huang YY, de Sousa MV, Bagnato VS, Parizotto NA, Hamblin MR (2015) Low-level laser (light) therapy increases mitochondrial membrane potential and ATP synthesis in C2C12 myotubes with a peak response at 3-6 h. Photochem Photobiol 91:411–416

    Article  CAS  PubMed  Google Scholar 

  3. De Marchi T, Leal Junior EC, Bortoli C, Tomazoni SS, Lopes-Martins RA, Salvador M (2012) Low-level laser therapy (LLLT) in human progressive-intensity running: effects on exercise performance, skeletal muscle status, and oxidative stress. Lasers Med Sci 27:231–236

    Article  PubMed  Google Scholar 

  4. Ferraresi C, Hamblin MR, Parizotto NA (2012) Low-level laser (light) therapy (LLLT) on muscle tissue: performance, fatigue and repair benefited by the power of light. Photonics Lasers Med 1:267–286

    Article  PubMed  PubMed Central  Google Scholar 

  5. Dos Reis FA, da Silva BA, Laraia EM, de Melo RM, Silva PH, Leal-Junior EC, de Carvalho PT (2014) Effects of pre- or post-exercise low-level laser therapy (830 nm) on skeletal muscle fatigue and biochemical markers of recovery in humans: double-blind placebo-controlled trial. Photomed Laser Surg 32:106–112

    Article  PubMed  Google Scholar 

  6. Samoilova KA, Zhevago NA, Petrishchev NN, Zimin AA (2008) Role of nitric oxide in the visible light-induced rapid increase of human skin microcirculation at the local and systemic levels: II. Healthy volunteers. Photomed Laser Surg 26:443–449

    Article  PubMed  Google Scholar 

  7. De Brito Vieira WH, Bezerra RM, Queiroz RA, Maciel NF, Parizotto NA, Ferraresi C (2014) Use of low-level laser therapy (808 nm) to muscle fatigue resistance: a randomized double-blind crossover trial. Photomed Laser Surg 32:678–685

    Article  PubMed  Google Scholar 

  8. Zagatto AM, Ramos SP, Nakamura FY, Lira FS, Lopes-Martins RAB, Carvalho RLP (2016) Effects of low-level laser therapy on performance, inflammatory markers and muscle damage in young water polo athletes: a double-blind, randomized, placebo-controlled study. Lasers Med Sci 31:511–521

    Article  PubMed  Google Scholar 

  9. Bertuzzi RC, Franchini E, Ugrinowitsch C, Kokubun E, Lima-Silva AE, Pires FO, Nakamura FY, Kiss MA (2010) Predicting MAOD using only a supramaximal exhaustive test. Int J Sports Med 31:477–481

    Article  CAS  PubMed  Google Scholar 

  10. Brisola GM, Miyagi WE, da Silva HS, Zagatto AM (2015) Sodium bicarbonate supplementation improved MAOD but is not correlated with 200- and 400-m running performances: a double-blind, crossover, and placebo-controlled study. Appl Physiol Nutr Metab 40:931–937

    Article  CAS  PubMed  Google Scholar 

  11. Milioni F, Malta ES, Rocha LGSA, Mesquita CAA, Freitas EC, Zagatto AM (2016) Acute administration of high doses of taurine does not substantially improve high-intensity running performance and the effect on maximal accumulated oxygen deficit is unclear. Appl Physiol Nutr Metab 41:498–503

    Article  CAS  PubMed  Google Scholar 

  12. Zagatto AM, Miyagi WE, Bertuzzi R, Papoti M (2016, In press) MAOD determined in a single supramaximal test: a study on the reliability and effects of supramaximal intensities. Int J Sports Med

  13. Zagatto AM, de Mello-Leite JV, Papoti M, Beneke R (2016, In press) Energetics of table tennis and table tennis specific exercise testing. Int J Sports Physiol Perform

  14. di Prampero PE, Ferretti G (1999) The energetics of anaerobic muscle metabolism: a reappraisal of older and recent concepts. Respir Physiol 118:103–115

    Article  PubMed  Google Scholar 

  15. Margaria R, Edwards HT, Dill DB (1933) The possible mechanisms of contracting and paying the oxygen debt and the role of lactic acid in muscular contraction. A J Physiol 106:689–715

    CAS  Google Scholar 

  16. Hill DW, Vingren JL (2014) Effects of exercise mode and participant sex on measures of anaerobic capacity. J Sports Med Phys Fitness 54:255–263

    CAS  PubMed  Google Scholar 

  17. Jurkowski JE, Jones NL, Toews CJ, Sutton JR (1981) Effects of menstrual cycle on blood lactate, O2 delivery, and performance during exercise. J Appl Physiol Respir Environ Exerc Physiol 51:1493–1499

    CAS  PubMed  Google Scholar 

  18. Jones AM, Doust JH (1996) A 1% treadmill grade most accurately reflects the energetic cost of outdoor running. J Sports Sci 14:321–327

    Article  CAS  PubMed  Google Scholar 

  19. Denis R, O’Brien C, Delahunt E (2013) The effects of light emitting diode therapy following high intensity exercise. Phys Ther Sport 14:110–115

    Article  PubMed  Google Scholar 

  20. Borg GA (1982) Psychophysical bases of perceived exertion. Med Sci Sports Exerc 14:377–381

    CAS  PubMed  Google Scholar 

  21. Yoon BK, Kravitz L, Robergs R (2007) VO2max, protocol duration, and the VO2 plateau. Med Sci Sports Exerc 39:1186–1192

    Article  PubMed  Google Scholar 

  22. Howley ET, Bassett DR Jr, Welch HG (1995) Criteria for maximal oxygen uptake: review and commentary. Med Sci Sports Exerc 27:1292–1301

    Article  CAS  PubMed  Google Scholar 

  23. Altman DG, Bland JM (1999) Statistics notes. Treatment allocation in controlled trials: why randomise? British Med J 318:1209

    Article  CAS  Google Scholar 

  24. Cohen J (1988) Statistical power analysis for the behavioral sciences. Lawrence Erlbaum, Hillsdale

    Google Scholar 

  25. Leal-Junior EC, Lopes-Martins RA, Baroni BM, De Marchi T, Rossi RP, Grosselli D, Generosi RA, de Godoi V, Basso M, Mancalossi JL, Bjordal JM (2009) Comparison between single-diode low-level laser therapy (LLLT) and LED multi-diode (cluster) therapy (LEDT) applications before high-intensity exercise. Photomed Laser Surg 27:617–623

    Article  PubMed  Google Scholar 

  26. Perini JL, Scotta Hentschke V, Sonza A, Dal Lago P (2016) Long-term low-level laser therapy promotes an increase in maximal oxygen uptake and exercise performance in a dose-dependent manner in Wistar rats. Lasers Med Sci 31:241–248

    Article  PubMed  Google Scholar 

  27. Leal-Junior EC, Lopes-Martins RA, Dalan F, Ferrari M, Sbabo FM, Generosi RA, Baroni BM, Penna SC, Iversen VV, Bjordal JM (2008) Effect of 655-nm low-level laser therapy on exercise-induced skeletal muscle fatigue in humans. Photomed Laser Surg 26:419–424

    Article  PubMed  Google Scholar 

  28. Leal-Junior EC, Vanin AA, Miranda EF, de Carvalho PT, Dal Corso S, Bjordal JM (2015) Effect of phototherapy (low-level laser therapy and light-emitting diode therapy) on exercise performance and markers of exercise recovery: a systematic review with meta-analysis. Lasers Med Sci 30:925–939

    Article  PubMed  Google Scholar 

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Funding for the study was provided by Foundation of Research Support of São Paulo State (FAPESP – protocols no. 2013/12940-8, 2016/02683-6, 2015/05012-2, 2016/02683-6 and 2015/05012-2).

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Correspondence to Alessandro Moura Zagatto.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Malta, E.D.S., De Poli, R.A.B., Brisola, G.M.P. et al. Acute LED irradiation does not change the anaerobic capacity and time to exhaustion during a high-intensity running effort: a double-blind, crossover, and placebo-controlled study. Lasers Med Sci 31, 1473–1480 (2016).

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