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Morphology, proliferation, and gene expression of gingival fibroblasts on Laser-Lok, titanium, and zirconia surfaces

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Abstract

Soft tissue seal plays a critical role in long-term success of dental implants, and the effects of implant surface treatments such as laser ablation have been a topic of particular interest in this respect. Considering the existing controversy regarding soft tissue behavior in contact with implant surfaces, this study sought to assess the morphology, proliferation, and gene expression of human gingival fibroblasts (HGFs) on different abutment surfaces. In this in vitro, experimental study, HGFs were cultured on 45 discs (Laser-Lok, titanium, and zirconia). Cell morphology, proliferation rate, and interleukin 10 (IL-10), tumor necrosis factor alpha (TNFα), fibronectin, and integrin gene expressions were assessed by electron microscopy, methyl thiazol tetrazolium (MTT) assay, and real-time polymerase chain reaction (PCR), respectively. Data were analyzed using ANOVA and the Kruskal–Wallis H test. Fibroblast attachment was noted in all the three groups. Spindle-shaped cells with pseudopod-like processes were more frequently seen in the Laser-Lok group. Cell proliferation was significantly higher in the Laser-Lok group compared to those in the other groups (P = 0.0002). Significant differences were found in the expression of IL-10, TNFα, fibronectin, and integrin genes among the groups (P < 0.01). Within the limitations of this study, HGFs on Laser-Lok surfaces had a more mature morphology and greater proliferation and differentiation as compared to those on zirconia and titanium surfaces. This indicates better attachment of these cells to laser-modified surfaces, creating a more efficient soft tissue seal around dental implants.

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Acknowledgment

The authors would like to express their gratitude to the Zarin Iran Company and BioHorizons, USA, for providing us with the discs.

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Authors and Affiliations

  1. Department of Periodontics, Dental Branch, Islamic Azad University, Tehran, Iran

    Nasrin Esfahanizadeh & Sara Motalebi

  2. Dental Implant Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Nasrin Esfahanizadeh

  3. 1501 Harvey Rd, #586, College Station, TX, 77840, USA

    Niloufar Daneshparvar

  4. Department of Mathematics, South Tehran Branch, Islamic Azad University, Tehran, Iran

    Nasrin Akhoundi

  5. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Shahin Bonakdar

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  1. Nasrin Esfahanizadeh
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  2. Sara Motalebi
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Correspondence to Niloufar Daneshparvar.

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Esfahanizadeh, N., Motalebi, S., Daneshparvar, N. et al. Morphology, proliferation, and gene expression of gingival fibroblasts on Laser-Lok, titanium, and zirconia surfaces. Lasers Med Sci 31, 863–873 (2016). https://doi.org/10.1007/s10103-016-1927-6

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  • DOI: https://doi.org/10.1007/s10103-016-1927-6

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