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The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation

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Abstract

The therapeutic effects of low-power laser radiation of different wavelengths and light doses are well known, but the biochemical mechanism of the interaction of laser light with living cells is not fully understood. We have investigated the effect of MLS (Multiwave Locked System) laser near-infrared irradiation on cell membrane structure, functional properties, and free radical generation using human red blood cells and breast cancer MCF-4 cells. The cells were irradiated with low-intensity MLS near-infrared (simultaneously 808 nm, continuous emission and 905 nm, pulse emission, pulse-wave frequency, 1,000 or 2,000 Hz) laser light at light doses from 0 to 15 J (average power density 212.5 mW/cm2, spot size was 3.18 cm2) at 22 °C, the activity membrane bound acetylcholinesterase, cell stability, anti-oxidative activity, and free radical generation were the parameters used in characterizing the structural and functional changes of the cell. Near-infrared low-intensity laser radiation changed the acetylcholinesterase activity of the red blood cell membrane in a dose-dependent manner: There was a considerable increase of maximal enzymatic rate and Michaelis constant due to changes in the membrane structure. Integral parameters such as erythrocyte stability, membrane lipid peroxidation, or methemoglobin levels remained unchanged. Anti-oxidative capacity of the red blood cells increased after MLS laser irradiation. This irradiation induced a time-dependent increase in free radical generation in MCF-4 cells. Low-intensity near-infrared MLS laser radiation induces free radical generation and changes enzymatic and anti-oxidative activities of cellular components. Free radical generation may be the mechanism of the biomodulative effect of laser radiation.

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Acknowledgments

Work funded by the Medical University of Lodz of the research task No. 502 -03/7-127-02/502-54-027.

Conflict of interest

No competing financial interests exist.

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Authors and Affiliations

  1. Clinic of Medical Rehabilitation, Medical University of Lodz, 75 Drewnowska Str., Lodz, 91-002, Poland

    Jolanta Kujawa & Kamila Pasternak

  2. Department of Biochemistry, Grodno State University named after Yanka Kupala, Blvd Len Kom - 50, Grodno, 230022, Belarus

    Ilya Zavodnik

  3. Laboratory of Ergonomics and Exercise Physiology, Medical University of Lodz, 1 Haller Sq., Lodz, 90-647, Poland

    Robert Irzmański

  4. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Str., Lodz, 90-237, Poland

    Dominika Wróbel

  5. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Str, Lodz, 90-236, Poland

    Maria Bryszewska

Authors
  1. Jolanta Kujawa
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  2. Kamila Pasternak
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  3. Ilya Zavodnik
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  4. Robert Irzmański
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  5. Dominika Wróbel
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  6. Maria Bryszewska
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Correspondence to Jolanta Kujawa.

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Kujawa, J., Pasternak, K., Zavodnik, I. et al. The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation. Lasers Med Sci 29, 1663–1668 (2014). https://doi.org/10.1007/s10103-014-1571-y

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  • DOI: https://doi.org/10.1007/s10103-014-1571-y

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