Lasers in Medical Science

, Volume 29, Issue 2, pp 773–779

Effects of LED phototherapy on relative wound contraction and reepithelialization during tissue repair in hypothyroid rats: morphometric and histological study

  • Gardênia Matos Paraguassú
  • Milena Góes da Guarda
  • Flávia Calo Aquino Xavier
  • Maria Cristina Teixeira Cangussu
  • Tânia Tavares Rodriguez
  • Maria José Pedreira Ramalho
  • Antônio Luis Barbosa Pinheiro
  • Luciana Maria Pedreira Ramalho
Original Article


The aim of this study was to assess morphometrically and histologically, the effects of light-emitting diode (LED) (λ630 ± 20 nm) phototherapy on reepithelialization and wound contraction during tissue repair in hypothyroid rats. Thyroid hormone deficiency has been associated with disorders of tissue repair. LED phototherapy has been studied using several healing models, but their usefulness in the improvement of hypothyroidism wound healing remains unknown. Under general anesthesia, a standard surgical wound (1 cm2) was produced on the dorsum of 48 male Wistar rats divided into four groups of 12 animals each: EC—control euthyroid, ED—euthyroid + LED, HC—control hypothyroid, and HD—Hypothyroid + LED. The irradiation started immediately after surgery and was repeated every other day for 7 and 14 days. Photographs of the wound were taken at the day of the surgical procedure and on days 8 and 15 after surgery, when animals’ deaths occurred. The specimens were removed, routinely processed, and stained with hematoxylin/eosin. Seven days after the surgery, it was possible to observe statistically significant reductions in the wound area of the irradiated euthyroid group, in comparison to hypothyroid group, irradiated and non-irradiated (ANOVA, p < 0.05). The reepithelialization was significantly higher in the euthyroid and hypothyroid groups irradiated with LED than in the non-irradiated groups (Fisher’s test, p < 0.05). No significant difference was found in the experimental period of 14 days among the groups. The hypothyroidism delayed wound healing and the LED phototherapy, at these specific parameters, improved the process of reepithelialization in the presence of hypothyroidism.


LED phototherapy Wound repair Reepithelialization Thyroid 


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Gardênia Matos Paraguassú
    • 1
  • Milena Góes da Guarda
    • 1
  • Flávia Calo Aquino Xavier
    • 1
  • Maria Cristina Teixeira Cangussu
    • 1
  • Tânia Tavares Rodriguez
    • 2
  • Maria José Pedreira Ramalho
    • 2
  • Antônio Luis Barbosa Pinheiro
    • 1
  • Luciana Maria Pedreira Ramalho
    • 1
  1. 1.School of Dentistry, Federal University of BahiaSalvadorBrazil
  2. 2.Department of PhysiologyHealth Sciences Institute, Federal University of BahiaSalvadorBrazil

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