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Controlling periodontal bone levels with multiple LED irradiations

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Abstract

Because a single exposure to light-emitting diode (LED) irradiation at 660 nm only demonstrated a 3-day biostimulatory effect in recovering periodontal bone level (PBL), this study sought to evaluate whether the periodontal effect could be extended through the use of multiple LED irradiations. Experimental periodontitis was developed unilaterally in 48 Sprague–Dawley rats after the placement of a silk ligature plus Porphyromonas gingivalis lipopolysaccharide injections. The animals were divided into four groups (no irradiation, a single irradiation, or two or three irradiations per week) and exposed to LED light irradiation at a wavelength of 660 ± 25 nm and energy density of 10 J/cm2 after debridement and detoxification. The animals were euthanized after 7 or 14 days, and the effect of irradiation was evaluated using micro-computed tomography and histology. By day 7, PBL was significantly reduced (p < 0.05), with significantly reduced inflammation (p < 0.05) and gingival hyperplasia (p < 0.001), in the animals receiving three irradiations per week. At day 14, the reduction in gingival hyperplasia was still significant (p < 0.05), and collagen matrix deposition and realignment appeared to be accelerated in the animals receiving three irradiations per week, despite a lack of significant difference in PBL. The treatment regimen receiving three LED light irradiations per week apparently extended the effects in reducing PBL and inflammation to 7 days. The inclusion of additional inflammation control measures or the addition of bioactive signals to mediate the repairing process is necessary to maintain long-term periodontal stability.

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Acknowledgments

The authors would like to acknowledge JETTS Technology Co., Ltd. (New Taipei, Taiwan) for fabricating the LED device and Man-Jung Kao at the National University of Singapore (NUS) for the assistance in animal care and micro-CT processing. The study was supported by research grants R-221-000-034-133 from NUS and 101R7465 from National Taiwan University.

Conflict of interest

The authors declare no financial conflicts of interest.

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Authors and Affiliations

  1. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei 100, Taiwan

    Po-Chun Chang

  2. Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan

    Po-Chun Chang & Chen-Ying Wang

  3. Faculty of Dentistry, National University of Singapore, Singapore, Singapore

    Po-Chun Chang & Li Yen Chong

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  1. Po-Chun Chang
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  2. Chen-Ying Wang
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Correspondence to Po-Chun Chang.

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Chang, PC., Wang, CY. & Chong, L.Y. Controlling periodontal bone levels with multiple LED irradiations. Lasers Med Sci 30, 741–746 (2015). https://doi.org/10.1007/s10103-013-1416-0

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  • DOI: https://doi.org/10.1007/s10103-013-1416-0

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