The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm2). Lasers had a power density of 1 W/cm2 and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm2 and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm2), 6.8 times by red laser (3 J/cm2), and 10.1 times by red laser (5 J/cm2) in relation to control group (p < 0.05). At the other periods, there was no influence of irradiation on cell growth (p > 0.05). The production of ALP was not influenced by irradiation at any period of time (p > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation.
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Fujihara NA, Hiraki KRN, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336
Damante CA, De Micheli G, Miyagi SPH, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24:885–891
Marques MM, Pereira AN, Fujihara NA, Nogueira FN, Eduardo CP (2004) Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. Lasers Surg Med 34:260–265
Maegawa Y, Itoh T, Hosokawa T, Yaegashi K, Nishi M (2000) Effects of near-infrared low-level laser irradiation on microcirculation. Lasers Surg Med 27:427–437
Damante CA, Greghi SLA, Sant’Ana ACP, Passanezi E (2004) Clinical evaluation of the effects of low intensity laser (GaAlAs) on wound healing after gingivoplasty in humans. J Appl Oral Sci 12(2):133–136
Damante CA, Greghi SLA, Sant’Ana ACP, Passanezi E, Taga R (2004) Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low-level laser therapy. Lasers Surg Med 35:377–384
Fujimoto K, Kiyosaki T, Mitsui N, Mayahara K, Omasa S, Suzuki N, Shimizu N (2010) Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers Surg Med 42:519–526
Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354
Haxsen V, Schikora D, Sommer U, Remppis A, Greten J, Kasperk C (2008) Relevance of laser irradiance threshold in the induction of alkaline phosphatase in human osteoblast cultures. Lasers Med Sci 23(4):381–384
Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–340
Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC (2003) Increased fibroblast proliferation induced by light emitting diode and lower power laser irradiation. Lasers Med Sci 18:95–99
Zambuzzi WF, Granjeiro JM, Parikh K, Yuvaraj S, Peppelenbosch MP, Ferreira CV (2008) Modulation of Src activity by low molecular weight protein tyrosine phosphatase during osteoblast differentiation. Cell Physiol Biochem 22(5–6):497–506
Sabino LG, de Negreiros LMV, Vollet-filho JD, Ferreira J, Tirapelli DPC, Novais PC, Tirapelli LF, Kurachi C, Bagnato VS (2011) Experimental evidence and model explanation for cell population characteristics modification when applying sequential photodynamic therapy. Las Phys Lett 8(3):239–246
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S, Bar I, Vago R (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20(3–4):138–146
Azevedo LH, Eduardo FP, Moreira MS, Eduardo CP, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth. A pilot study. Lasers Med Sci 21:86–89
Li WT, Leu YC, Wu JL (2010) Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg 28(1):157–165
Souza APC, Santos JN, Reis J Jr, Ramos TA, Souza J, Cangussu MCT, Pinheiro ALB (2010) Effect of LED phototherapy of three distinct wavelengths on fibroblasts on wound healing: a histological study in a rodent model. Photomed Laser Surg 28(4):547–552
McDaniel, Weiss RA, Geronemus MD, Mazur BS, Wilson MS, Weiss MA (2010) Varying ratios of wavelengths in dual wavelength led photomodulation alters gene expression profiles in human skin fibroblasts. Lasers Surg Med 42:540–545
Whelan HT, Smits RL Jr, Buchman EV, Whelan NT, Turner SG, Margolis DA, Cevenini V, Stinson H, Ignatius R, Martin T, Cwiklinski J, Philippi AF, Graf WR, Hodgson B, Gould L, Kane M, Chen G, Caviness J (2001) Effect of NASA light-emitting diode irradiation on wound healing. J Clin Laser Med Surg 19(6):305–314
Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166
Cankaya AB, Erdem MA, Erdem AP, Erguven M, Aybar B, Kasapoglu C, Bilir A (2011) Evaluation of light-emitting diode (LED-660 nm) application over primary osteoblast-like cells on titanium surfaces: an in vitro study. Int J Med Sci 8(7):584–593
Kim HK, Kim JH, Abbas AA, Kim DO, Park SJ, Chung JY, Song EK, Yoon TR (2009) Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24(2):214–222
Dall Agnol MA, Nicolau RA, de Lima CJ, Munin E (2009) Comparative analysis of coherent light action (laser) versus non-coherent light (light-emitting diode) for tissue repair in diabetic rats. Lasers Med Sci 24(6):909–916
Quarles LD, Yohay LD, Lever LW, Caton R, Wenstrup RJ (1992) Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7:683–692
Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64:295–312
Hirata S, Kitamura C, Fukushima H, Nakamichi I, Abiko Y, Terashita M, Jimi E (2010) Low-level laser irradiation enhances BMP-induced osteoblast differentiation by stimulating the BMP/Smad signaling pathway. J Cell Biochem 111(6):1445–1452
Peng F, Wu H, Zheng Y, Xu X, Yu J (2012) The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Lasers Med Sci 27(3):645–653
Horvát-Karajz K, Balogh Z, Kovács V, Drrernat AH, Sréter L, Uher F (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41:463–469
The authors would like to thank Prof. Dr. Heitor Marques Honório for statistical analysis. This research was granted by the Fundação de amparo à pesquisa do estado de São Paulo, # 2010/18361-1.
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Pagin, M.T., de Oliveira, F.A., Oliveira, R.C. et al. Laser and light-emitting diode effects on pre-osteoblast growth and differentiation. Lasers Med Sci 29, 55–59 (2014). https://doi.org/10.1007/s10103-012-1238-5
- Wound healing
- Bone regeneration