Lasers in Medical Science

, Volume 27, Issue 5, pp 923–933 | Cite as

Short-term nanostructural effects of high radiofrequency treatment on the skin tissues of rabbits

  • Samjin Choi
  • Youjin Cheong
  • Jae-Ho Shin
  • Hui-Jae Lee
  • Gi-Ja Lee
  • Seok Keun Choi
  • Kyung-Hyun Jin
  • Hun-Kuk ParkEmail author
Original Article


The aim of this study is to quantitatively investigate the short-term effects of RF tissue-tightening treatment in in vivo rabbit dermal collagen fibrils. These effects were measured at different energy levels and at varying pass procedures on the nanostructural response level using histology and AFM analysis. Each rabbit was divided into one of seven experimental groups, which included the following: control group, and six RF group according to RF energy (20 W and 40 W) and three RF pass procedures. The progressive changes in the diameter and D-periodicity of rabbit dermal collagen fibrils were investigated in detail over a 7-day post-treatment period. The dermal tissues treated with the RF tissue-tightening device showed more prominent inflammatory responses with inflammatory cell ingrowth compared to the control. This effect showed more prominent with the passage of day after treatment. Although an increase in the diameter and D-periodicity of dermal collagen fibrils was identified immediately after the RF treatment, a decrease in the morphology of dermal collagen fibrils continued until post-operative day 7. Furthermore, RF treatment led to the loss of distinct borders. Increases in RF energy with the same pass procedure, as well as an increase in the number of RF passes, increased the occurrence of irreversible collagen fibril injury. A multiple-pass treatment at low energy rather than a single-pass treatment at high energy showed a large amount of collagen fibrils contraction at the nanostructural level.


High radiofrequency treatment Energy and pass procedure Dermal collagen fibrils Inflammatory response Atomic force microscopy 



This study was supported by the Bio R&D program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (grant 2009–0092562).


  1. 1.
    Kerscher M (2009) Aesthetic and cosmetic dermatology. Eur J Dermatol 19:530–534PubMedGoogle Scholar
  2. 2.
    Kushikata N, Negishi K, Tezuka Y, Takeuchi K, Wakamatsu S (2005) Non-ablative skin tightening with radiofrequency in Asian skin. Lasers Surg Med 36:92–97PubMedCrossRefGoogle Scholar
  3. 3.
    Zelickson BD, Kist D, Bernstein E, Brown DB, Ksenzenko S, Burns J, Kilmer S, Mehregan D, Pope K (2004) Histological and ultrastructural evaluation of the effects of a radiofrequency-based nonablative dermal remodeling device: a pilot study. Arch Dermatol 140:204–209PubMedCrossRefGoogle Scholar
  4. 4.
    Kist D, Burns AJ, Sanner R, Counters J, Zelickson B (2006) Ultrastructural evaluation of multiple-pass low-energy versus single-pass high-energy radio-frequency treatment. Lasers Surg Med 38:150–154PubMedCrossRefGoogle Scholar
  5. 5.
    Atiyeh BS, Dibo SA (2009) Nonsurgical nonablative treatment of aging skin: radiofrequency technologies between aggressive marketing and evidence-based efficacy. Aesthetic Plast Surg 33:283–294PubMedCrossRefGoogle Scholar
  6. 6.
    Sadick NS (2003) Update on non-ablative light therapy for rejuvenation: a review. Lasers Surg Med 32:120–128PubMedCrossRefGoogle Scholar
  7. 7.
    Hardaway CA, Ross EV, Paithankar DY (2002) Non-ablative cutaneous remodeling with a 1.45-micron mid-infrared diode laser: phase II. J Cosmet Laser Ther 4:9–14PubMedCrossRefGoogle Scholar
  8. 8.
    Yamauchi PS, Lowe NJ (2004) Botulinum toxin types A and B: comparison of efficacy, duration, and dose-ranging studies for the treatment of facial rhytides and hyperhidrosis. Clin Dermatol 22:34–39PubMedCrossRefGoogle Scholar
  9. 9.
    Naoum C, Dasiou-Plakida D (2001) Dermal filler materials and botulin toxin. Int J Dermatol 40:609–621PubMedCrossRefGoogle Scholar
  10. 10.
    England LJ, Tan MH, Shumaker PR, Egbert BM, Pittelko K, Orentreich D, Pope K (2005) Effects of monopolar radiofrequency treatment over soft-tissue fillers in an animal model. Lasers Surg Med 37:356–365PubMedCrossRefGoogle Scholar
  11. 11.
    Alster TS, Tanzi E (2004) Improvement of neck and cheek laxity with a nonablative radiofrequency device: a lifting experience. Dermatol Surg 30:503–507PubMedCrossRefGoogle Scholar
  12. 12.
    Ruiz-Esparza J, Gomez JB (2003) The medical face lift: a noninvasive, nonsurgical approach to tissue tightening in facial skin using nonablative radiofrequency. Dermatol Surg 29:325–332PubMedCrossRefGoogle Scholar
  13. 13.
    Hsu TS, Kaminer MS (2003) The use of nonablative radiofrequency technology to tighten the lower face and neck. Semin Cutan Med Surg 22:115–123PubMedCrossRefGoogle Scholar
  14. 14.
    Fritz M, Counters JT, Zelickson BD (2004) Radiofrequency treatment for middle and lower face laxity. Arch Facial Plast Surg 6:370–373PubMedCrossRefGoogle Scholar
  15. 15.
    Lyer S, Suthamjariya K, Fitzpatrick RE (2003) Using a radiofrequency energy device to treat the lower face: a treatment paradigm for a nonsurgical facelift. Cosmet Dermatol 16:37–40Google Scholar
  16. 16.
    Friedman DJ, Gilead LT (2007) The use of hybrid radiofrequency device for the treatment of rhytides and lax skin. Dermatol Surg 33:543–551PubMedCrossRefGoogle Scholar
  17. 17.
    Narins RS, Tope WD, Pope K, Ross EV (2006) Overtreatment effects associated with radiofrequency tissue-tightening device: rare, preventable and correctable with subcision and autologous fat transfer. Dermatol Surg 32:115–124PubMedCrossRefGoogle Scholar
  18. 18.
    Binnig G, Quate CF, Gerber C (1986) Atomic force microscope. Phys Rev Lett 56:930–933PubMedCrossRefGoogle Scholar
  19. 19.
    Xu M, Fujita D, Onishi K, Miyazawa K (2009) Improving accuracy of sample surface topography by atomic force microscopy. J Nanosci Nanotechnol 9:6003–6007PubMedCrossRefGoogle Scholar
  20. 20.
    Choi S, Park KH, Cheong Y, Kim HK, Park YG, Park HK (2011) Changes in ultrastructure and properties of bracket slots after orthodontic treatment with bicuspid extraction. Scanning 33:25–32PubMedCrossRefGoogle Scholar
  21. 21.
    Choi S, Lee SJ, Shin JH, Cheong Y, Lee HJ, Paek JH, Kim JS, Jin KH, Park HK (2011) Ultrastructural investigation of intact orbital implant surfaces using atomic force microscopy. Scanning 33:211–221PubMedGoogle Scholar
  22. 22.
    Choi S, Shin JH, Cheong Y, Lee HJ, Jin KH, Park HK (2011) Nanostructural investigation of frontalis sling biomaterial surfaces. Scanning. doi: 10.1002/sca.20233
  23. 23.
    Choi S, Rhee Y, Park JH, Lee GJ, Kim KS, Park JH, Park YG, Park HK (2010) Effects of fluoride treatment on phosphoric acid-etching in primary teeth: an AFM observation. Micron 41:498–506PubMedCrossRefGoogle Scholar
  24. 24.
    Choi S, Cheong Y, Lee GJ, Park HK (2010) Effect of fluoride pretreatment on primary and permanent tooth surfaces by acid-etching. Scanning 32:375–382PubMedCrossRefGoogle Scholar
  25. 25.
    Choi S, Cheong Y, Lee HJ, Lee SJ, Jin KH, Park HK (2011) AFM study for morphological and material property of human sclera surface. J Nanosci Nanotechnol 11:6382–6388PubMedCrossRefGoogle Scholar
  26. 26.
    Thorsteinsdottir S, Deries M, Cachaco AS, Bajanca F (2011) The extracellular matrix dimension of skeletal muscle development. Dev Biol 354:191–207PubMedCrossRefGoogle Scholar
  27. 27.
    Helfrich YR, Sachs DL, Voorhees JJ (2008) Overview of skin aging and photoaging. Dermatol Nurs 20:177–183PubMedGoogle Scholar
  28. 28.
    Holck DE, Ng JD (2003) Facial skin rejuvenation. Curr Opin Ophthalmol 14:246–252PubMedCrossRefGoogle Scholar
  29. 29.
    Carneiro SC, Cassia Fde F, Pascarelli BM, Souza SO, Ramos-e-Silva M, Filgueira AL, Japiassu MA, Takiya CM (2007) Increase in dermal collagen fibril diameter and elastogenesis with UVB exposure: an optical and ultrastructural study in albino Balb/c mice. Acta Dermatovenerol Croat 15:65–71PubMedGoogle Scholar
  30. 30.
    Boisnic S, Branchet MC, Birnstiel O, Beilin G (2010) Clinical and histopathological study of the TriPollar home-use device for body treatments. Eur J Dermatol 20:367–372PubMedGoogle Scholar
  31. 31.
    Hsu TS, Kaminer MS (2003) The use of nonablative radiofrequency technology to tighten the lower face and neck. Semin Cutan Med Surg 22:115–123PubMedCrossRefGoogle Scholar
  32. 32.
    Tzaphlidou M (2001) Diameter distributions of collagenous tissues in relation to sex. A quantitative ultrastructural study. Micron 32:333–336PubMedCrossRefGoogle Scholar
  33. 33.
    Alaseirlis DA, Li Y, Cilli F, Fu FH, Wang JHC (2005) Decreasing inflammatory response of injured patellar tendons results in increased collagen fibril diameters. Connect Tissue Res 46:12–7PubMedCrossRefGoogle Scholar
  34. 34.
    Williams IF, McCullagh KG, Goodship AE, Silver IA (1984) Studies on the pathogenesis of equine tendonitis following collagenase injury. Res Vet Sci 36:326–338PubMedGoogle Scholar
  35. 35.
    Williams IF, McCullagh KG, Silver IA (1984) The distribution of types I and III collagen and fibronectin in the healing equine tendon. Connect Tissue Res 2:211–227CrossRefGoogle Scholar
  36. 36.
    Langsjo TK, Rieppo J, Pelttari A, Oksala N, Kovanen V, Helminen HJ (2002) Collagenase-induced changes in articular cartilage as detected by electron-microscopic stereology, quantitative polarized light microscopy and biochemical assays. Cells Tissues Organs 172:265–275PubMedCrossRefGoogle Scholar
  37. 37.
    Sharma PMN (2006) Biology of tendon injury: healing, modeling and remodeling. J Musculoskelet Neuronal Interact 6:181–190PubMedGoogle Scholar
  38. 38.
    Aström M, Rausing A (1995) Chronic Achilles tendinopathy. A survey of surgical and histopathologic findings. Clin Orthop Relat Res 316:151–164PubMedGoogle Scholar

Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Samjin Choi
    • 1
    • 2
  • Youjin Cheong
    • 1
  • Jae-Ho Shin
    • 3
  • Hui-Jae Lee
    • 3
  • Gi-Ja Lee
    • 1
  • Seok Keun Choi
    • 4
  • Kyung-Hyun Jin
    • 3
  • Hun-Kuk Park
    • 1
    • 5
    Email author
  1. 1.Department of Biomedical Engineering & Healthcare Industry Research Institute, College of MedicineKyung Hee UniversitySeoulSouth Korea
  2. 2.Department of Orthodontics, College of Dental MedicineKyung Hee UniversitySeoulSouth Korea
  3. 3.Department of Ophthalmology, College of MedicineKyung Hee UniversitySeoulSouth Korea
  4. 4.Department of Neurosurgery, College of MedicineKyung Hee UniversitySeoulSouth Korea
  5. 5.Program of Medical EngineeringKyung Hee UniversitySeoulSouth Korea

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