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Lasers in Medical Science

, Volume 27, Issue 5, pp 923–933 | Cite as

Short-term nanostructural effects of high radiofrequency treatment on the skin tissues of rabbits

  • Samjin Choi
  • Youjin Cheong
  • Jae-Ho Shin
  • Hui-Jae Lee
  • Gi-Ja Lee
  • Seok Keun Choi
  • Kyung-Hyun Jin
  • Hun-Kuk ParkEmail author
Original Article

Abstract

The aim of this study is to quantitatively investigate the short-term effects of RF tissue-tightening treatment in in vivo rabbit dermal collagen fibrils. These effects were measured at different energy levels and at varying pass procedures on the nanostructural response level using histology and AFM analysis. Each rabbit was divided into one of seven experimental groups, which included the following: control group, and six RF group according to RF energy (20 W and 40 W) and three RF pass procedures. The progressive changes in the diameter and D-periodicity of rabbit dermal collagen fibrils were investigated in detail over a 7-day post-treatment period. The dermal tissues treated with the RF tissue-tightening device showed more prominent inflammatory responses with inflammatory cell ingrowth compared to the control. This effect showed more prominent with the passage of day after treatment. Although an increase in the diameter and D-periodicity of dermal collagen fibrils was identified immediately after the RF treatment, a decrease in the morphology of dermal collagen fibrils continued until post-operative day 7. Furthermore, RF treatment led to the loss of distinct borders. Increases in RF energy with the same pass procedure, as well as an increase in the number of RF passes, increased the occurrence of irreversible collagen fibril injury. A multiple-pass treatment at low energy rather than a single-pass treatment at high energy showed a large amount of collagen fibrils contraction at the nanostructural level.

Keywords

High radiofrequency treatment Energy and pass procedure Dermal collagen fibrils Inflammatory response Atomic force microscopy 

Notes

Acknowledgments

This study was supported by the Bio R&D program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (grant 2009–0092562).

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Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Samjin Choi
    • 1
    • 2
  • Youjin Cheong
    • 1
  • Jae-Ho Shin
    • 3
  • Hui-Jae Lee
    • 3
  • Gi-Ja Lee
    • 1
  • Seok Keun Choi
    • 4
  • Kyung-Hyun Jin
    • 3
  • Hun-Kuk Park
    • 1
    • 5
    Email author
  1. 1.Department of Biomedical Engineering & Healthcare Industry Research Institute, College of MedicineKyung Hee UniversitySeoulSouth Korea
  2. 2.Department of Orthodontics, College of Dental MedicineKyung Hee UniversitySeoulSouth Korea
  3. 3.Department of Ophthalmology, College of MedicineKyung Hee UniversitySeoulSouth Korea
  4. 4.Department of Neurosurgery, College of MedicineKyung Hee UniversitySeoulSouth Korea
  5. 5.Program of Medical EngineeringKyung Hee UniversitySeoulSouth Korea

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