Lasers in Medical Science

, Volume 27, Issue 3, pp 607–614 | Cite as

Sensitivity of A-549 human lung cancer cells to nanoporous zinc oxide conjugated with Photofrin

  • Muhammad Fakhar-e-Alam
  • Syed Muhammad Usman Ali
  • Zafar Hussain Ibupoto
  • Khun Kimleang
  • M. Atif
  • Muhammad Kashif
  • Foo Kai Loong
  • Uda Hashim
  • Magnus Willander
Original Article

Abstract

In the present study, we demonstrated the use of nanoporous zinc oxide (ZnO NPs) in photodynamic therapy. The ZnO NPs structure possesses a high surface to volume ratio due to its porosity and ZnO NPs can be used as an efficient photosensitizer carrier system. We were able to grow ZnO NPs on the tip of borosilicate glass capillaries (0.5 μm diameter) and conjugated this with Photofrin for efficient intracellular drug delivery. The ZnO NPs on the capillary tip could be excited intracellularly with 240 nm UV light, and the resultant 625 nm red light emitted in the presence of Photofrin activated a chemical reaction that produced reactive oxygen species (ROS). The procedure was tested in A-549 cells and led to cell death within a few minutes. The morphological changes in necrosed cells were examined by microscopy. The viability of control and treated A-549 cells with the optimum dose of UV/visible light was assessed using the MTT assay, and ROS were detected using a fluorescence microscopy procedure.

Keywords

Lung cancer (A-549) cells MTT assay Photofrin Cell viability Nanoporous zinc oxide (ZnO NPs) Reactive oxygen species (ROS) Photodynamic therapy (PDT) 

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Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Muhammad Fakhar-e-Alam
    • 1
    • 2
  • Syed Muhammad Usman Ali
    • 1
    • 3
  • Zafar Hussain Ibupoto
    • 1
  • Khun Kimleang
    • 1
  • M. Atif
    • 4
    • 6
  • Muhammad Kashif
    • 5
  • Foo Kai Loong
    • 5
  • Uda Hashim
    • 5
  • Magnus Willander
    • 1
  1. 1.Department of Science and TechnologyCampus Norrköping, Linköping UniversityNorrköpingSweden
  2. 2.Pakistan Institute of Engineering and Applied SciencesNilorePakistan
  3. 3.Department of Electronic EngineeringNED University of Engineering and TechnologyKarachiPakistan
  4. 4.Laser Diagnosis of Cancer, Physics and Astronomy Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  5. 5.Nano Biochip Research Group, Institute of Nano Electronic Engineering (INEE)University Malaysia Perlis (UniMAP)KangarMalaysia
  6. 6.National Institute of Laser and OptronicsNilorePakistan

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