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Skin pretreatment with lasers promotes the transdermal delivery of vitamin C derivatives

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Abstract

The objective of this study is to investigate the effects of two lasers (erbium:YAG and CO2) on the ability to enhance skin permeation of two vitamin C derivatives, 3-O-ethyl ascorbic acid (EAC) and ascorbic acid 2-glucoside (AA2G). The study was taken in the skin of a female nude mouse (Balb/c-nu strain, 8 weeks old) in vitro. The histologic and ultrastructural changes of the nude mouse skin treated by the lasers were examined under light microscopy and transmission electron microscopy, respectively. The in vitro permeation of vitamin C derivatives was performed in Franz cell. The stratum corneum (SC) layer in the skin was partly ablated by erbium:YAG laser treatment, resulting in greater permeation of both vitamin C derivatives. The flux of EAC and AA2G across erbium:YAG laser-treated skin was 105 to 189-fold and 35 to 78-fold higher, respectively, than their flux across intact skin. The increase in enhancement ratio with increase in fluency decreases markedly for both compounds at the last dose escalation (from 5.0 to 6.3 J/cm2). Both SC ablation and a thermal effect may contribute to the effect of the CO2 laser on skin structure. The flux of EAC and AA2G across CO2 laser-treated skin was 181 to 277-fold and 82 to 117-fold higher, respectively, than their flux across intact skin. We concluded that both erbium:YAG and CO2 laser pretreatment increased the transdermal flux of two stable vitamin C derivatives, EAC and AA2G. The optimal fluency for the Er:YAG laser was 5 J/cm2.

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References

  1. Lee WR, Shen SC, Wang KH, Hu CH, Fang JY (2002) The effect of laser treatment on skin to enhance and control transdermal delivery of 5-fluorouracil. J Pharm Sci 91:1613–1626

    Article  PubMed  CAS  Google Scholar 

  2. Alexiades-Armenakas MR, Dover JS, Arndt KA (2008) The spectrum of laser skin resurfacing: nonablative, fractional, and ablative resurfacing. J Am Acad Dermatol 58:738–740

    Article  Google Scholar 

  3. Reszko AE, Bereson D, Lupo MP (2009) Cosmeceuticals: practical applications. Dermatol Clin 27:401–416

    PubMed  CAS  Google Scholar 

  4. Lee WR, Shen SC, Lai HH, Hu CH, Fang JY (2001) Transdermal drug delivery enhanced and controlled by erbium:YAG laser: a comparative study of lipophilic and hydrophilic drugs. J Control Rel 75:155–166

    Article  CAS  Google Scholar 

  5. Humbert PG, Haftek M, Creidi P, Lapiere C, Nusgens B, Richard A, Schmidt D, Zahouani H (2003) Topical ascorbic acid on photoaged skin. Clinical topographical and ultrastructural evaluation: double-blind study vs. placebo. Exp Dermatol 12:237–244

    Article  PubMed  CAS  Google Scholar 

  6. Traikovich SS (1999) Use of topical ascorbic acid and its effects on photodamaged skin topography. Arch Otolaryngol Head Neck Surg 125:1091–1098

    PubMed  CAS  Google Scholar 

  7. Espinal-Perez LE, Moncada B, Castanedo-Casares JP (2004) A double-blind randomized trial of 5% vitamin C vs. 4% hydroquinone in melasma. Int J Dermatol 4:604–607

    Article  Google Scholar 

  8. Fitzpatrick RE, Rostan EF (2002) Double-blind, half-face study comparing topical vitamin C and vehicle for rejuvenation of photodamage. Dermatol Surg 28:231–236

    Article  PubMed  Google Scholar 

  9. Lee WR, Shen SC, Wang KH, Hu CH, Fang JY (2003) Lasers and microdermabrasion enhance and control topical delivery of vitamin C. J Invest Dermatol 121:1118–1125

    Article  PubMed  CAS  Google Scholar 

  10. Gomez C, Costela A, Garcia-Moreno I, Llanes F, Teijon JM, Blanco D (2008) Laser treatments on skin enhancing and controlling transdermal delivery of 5-fluorouracil. Lasers Surg Med 40:6–12

    Article  PubMed  Google Scholar 

  11. Fang JY, Lee WR, Shen SC, Wang HY, Fang CL, Hu CH (2004) Transdermal delivery of macromolecules by erbium:YAG laser. J Control Rel 100:75–85

    Article  CAS  Google Scholar 

  12. Riggs K, Keller M, Humphreys TR (2007) Ablative laser resurfacing: high-energy pulsed carbon dioxide and erbium:yttrium-aluminum-garnet. Clin Dermatol 25(5):462–473

    Article  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the Microscopy Core Laboratory of Chang Gung Memorial Hospital.

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Authors and Affiliations

  1. Department of Nutrition and Health Sciences, Chang Gung Institute of Technology, Taoyuan, 333, Taiwan

    Chien-Yu Hsiao

  2. School of Nutrition and Health Sciences, Taipei Medical University, 250 Wuxing Street, Taipei, 110, Taiwan

    Chien-Yu Hsiao & Shih-Yi Huang

  3. Department of Cosmetic Science, Chang Gung Institute of Technology, Taoyuan, 333, Taiwan

    Chun-Hsun Huang

  4. Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Taoyuan, 333, Taiwan

    Sindy Hu

  5. First Cardiovascular Division, Department of Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan

    Yu-Shien Ko

  6. College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan

    Yu-Shien Ko

  7. Department of Anatomy, Chang Gung University, Taoyuan, 333, Taiwan

    Hsin-Ching Sung

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  1. Chien-Yu Hsiao
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  2. Chun-Hsun Huang
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  3. Sindy Hu
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  4. Yu-Shien Ko
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  6. Shih-Yi Huang
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Correspondence to Shih-Yi Huang.

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Hsiao, CY., Huang, CH., Hu, S. et al. Skin pretreatment with lasers promotes the transdermal delivery of vitamin C derivatives. Lasers Med Sci 26, 369–376 (2011). https://doi.org/10.1007/s10103-010-0863-0

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  • DOI: https://doi.org/10.1007/s10103-010-0863-0

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