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Expression of receptor activator of nuclear factor -κB ligand, receptor activator of nuclear factor -κB, and osteoprotegerin, following low-level laser treatment on deproteinized bovine bone graft in rats

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Abstract

The aim of this study was to investigate by immunohistochemistry the effects of low-level laser (LLL) irradiation on the expression of the receptor activator of nuclear factor -κB ligand (RANKL), osteoprotegerin (OPG), and the receptor activator of nuclear factor -κB (RANK) in deproteinized bovine bone grafts in rats. Twenty-four male Sprague-Dawley rats aged 15 weeks were allocated to either an experimental group that underwent LLL irradiation during bone healing at the bone graft sites of the rats’ calvarial bone defects or a control group. In the experimental group, gallium–aluminum–arsenide (Ga-Al-As) diode LLL (wavelength 808 nm; output 96 mW) was used to irradiate three areas on and around bone defects. The radiation was administered by the contact method for 10 s at 8.3 J/cm2, once a day for 7 days. The total dose over the complete schedule was 40.32 J. The animals were killed on days 7, 14 or 21. The results of immunohistochemical analysis showed that the expression of RANKL (P = 0.199), OPG (P = 0.035), and RANK (P = 0.020) in the experimental group significantly increased from day 7, with a more even distribution than in the control group, and that this difference prevailed until the end of the experiment. Bone density of the experimental group after trichrome staining was also higher than in the control group. These results suggest that LLL irradiation facilitates bone metabolism during bone healing at the sites of deproteinized bovine bone grafts in rats.

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References

  1. Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39. doi:10.1002/lsm.1900050105

    Article  PubMed  CAS  Google Scholar 

  2. Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503–3509. doi:10.1016/j.biomaterials.2004.09.033

    Article  PubMed  CAS  Google Scholar 

  3. Kim YD, Kim SS, Hwang DS, Kim SG, Kwon YH, Shin SH et al (2007) Effect of low-level laser treatment after installation of dental titanium implant-immunohistochemical study of RANKL, RANK, OPG: an experimental study in rats. Lasers Surg Med. 39:441–450 doi:10.1002/lsm.20508

    Article  PubMed  Google Scholar 

  4. Kim YD, Kim SS, Hwang DS, Kim GC, Shin SH, Kim UK et al (2007) Effect of low-level laser treatment after installation of dental titanium implant-immunohistochemical study of vascular endothelial growth factor: an experimental study in rats. Laser Phys Lett 4:681–685. doi:10.1002/lapl.200710036

    Article  Google Scholar 

  5. Pinheiro ALB, Gerbi MEMM (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24:169–178. doi:10.1089/pho.2006.24.169

    Article  PubMed  CAS  Google Scholar 

  6. Pinheiro ALB, Limeira Júnior FA, Gerbi MEMM et al (2003) Effect of 830 nm laser light on the repair of bone defects grafted with inorganic bovine bone and decalcified cortical osseous membrane. J Clin Laser Med Surg 21:383–388. doi:10.1089/104454703322650202

    Article  Google Scholar 

  7. Gerbi ME, Pinheiro AL, Marzola C et al (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382–388. doi:10.1089/pho.2005.23.382

    Article  PubMed  Google Scholar 

  8. Gerbi ME, Marques AM, Ramalho LM et al (2008) Infrared laser light further improves bone healing when associated with bone morphogenic proteins: an in vivo study in a rodent model. Photomed Laser Surg 26:55–60. doi:10.1089/pho.2007.2026

    Article  PubMed  Google Scholar 

  9. Khadra M, Kassem N, Haanæs HR, Ellingsen JE, Lyngstadaas SP (2004) Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:693–700. doi:10.1016/j.tripleo.2003.11.008

    Article  PubMed  Google Scholar 

  10. Matsumoto MA, Ferino RV, Monteleone GF, Ribeiro DA (2008) Low-level laser therapy modulates cyclo-oxygenase-2 expression during bone repair in rats. Lasers Med Sci (in press). doi:10.1007/s10103–008–0544–4

  11. Smith RC, Baker KS (1981) Optical properties of the clearest natural waters (200–800 nm). Appl Opt 20:177–184

    Article  Google Scholar 

  12. Mester E, Spiry T, Szende B, Tota JG (1971) Effect of laser rays on wound healing. Am J Surg 122:532–535. doi:10.1016/0002–9610(71)90482-X

    Article  PubMed  CAS  Google Scholar 

  13. Khadra M, Rønold HJ, Lyngstadaas SP, Ellingsen JE, Haanæs HR (2004) Low-level laser therapy stimulates bone-implant interaction: an experimental study in rabbits. Clin Oral Implants Res 15:325–332. doi:10.1111/j.1600–0501.2004.00994.x

    Article  PubMed  Google Scholar 

  14. Khadra M, Kasem N, Lyngstadaas SP, Haanæs HR, Mustafa K (2005) Laser therapy accelerates initial attachment and subsequent behaviour of human oral fibroblasts cultured on titanium implant material. A scanning electron microscopic and histomorphometric analysis. Clin Oral Implants Res 16:168–175. doi:10.1111/j.1600–0501.2004.01092.x

    Article  PubMed  Google Scholar 

  15. Kert J, Rose L (1989) Clinical laser therapy-low level laser therapy laser therapy. Scandinavian Medical Laser Technology, Ballerup, Denmark

    Google Scholar 

  16. Saperia D, Glassberg E, Lyons AF et al (1986) Demonstration of elevated type I and type III procollagen mRNA levels in cutaneous wounds treated with helium-neon laser. Proposed mechanism for enhanced wound healing. Biophys Res Commun 138:1123–1128. doi:10.1016/S0006–291X(86)80399–0

    Article  CAS  Google Scholar 

  17. Roodman GD (1999) Cell biology of the osteoclast. Exp Hematol 27:1229–1241. doi:10.1016/S0301–472X(99)00061–2

    Article  PubMed  CAS  Google Scholar 

  18. Kodama H, Nose M, Niida S, Yamasaki A (1991) Essential role of macrophage colony-stimulating factor in the osteoclast differentiation supported by stromal cells. J Exp Med 173:1291–1294. doi:10.1084/jem.173.5.1291

    Article  PubMed  CAS  Google Scholar 

  19. Yasuda H, Shima N, Nakagawa N et al (1998) Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANK. Proc Natl Acad Sci U S A 95:3597–3602. doi:10.1073/pnas.95.7.3597

    Article  PubMed  CAS  Google Scholar 

  20. Hofbauer LC, Heufelder AE (1998) Osteoprotogerin and its cognate ligand: a new paradigm of osteoclastogenesis. Eur J Endocrinol 139:152–154. doi:10.1530/eje.0.1390152

    Article  PubMed  CAS  Google Scholar 

  21. Hofbauer LC, Heufelder AE (2001) Role of receptor activator of nuclear factor-κB ligand and osteoprotegerin in bone cell biology. J Mol Med 79:243–253. doi:10.1007/s001090100226

    Article  PubMed  CAS  Google Scholar 

  22. Udagawa N et al (2000) Osteoprotegerin produced by osteoblasts is an important regulator in osteoclast development and function. Endocrinology 141:3478–3484. doi:10.1210/en.141.9.3478

    Article  PubMed  CAS  Google Scholar 

  23. Kim YD, Kim SS, Kim TG, Kim GC, Park SB, Son WS (2007) Effect of low level laser treatment during tooth movement-immunohistochemical study of RANKL, RANK, OPG: an experimental study in rats. Laser Phys Lett 4:616–623. doi:10.1002/lapl.200710032

    Article  CAS  Google Scholar 

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Correspondence to Seong-Sik Kim.

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Kim, YD., Song, WW., Kim, SS. et al. Expression of receptor activator of nuclear factor -κB ligand, receptor activator of nuclear factor -κB, and osteoprotegerin, following low-level laser treatment on deproteinized bovine bone graft in rats. Lasers Med Sci 24, 577–584 (2009). https://doi.org/10.1007/s10103-008-0614-7

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  • DOI: https://doi.org/10.1007/s10103-008-0614-7

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