Abstract
Nitrogen laser-induced fluorescence (LIF) and tungsten halogen lamp excited diffuse reflectance spectra were recorded in 350- to 700-nm range on a miniature fiber-optic spectrometer from in vitro premolar tooth during various stages of artificial erosion with 36% phosphoric acid. Both the LIF spectral intensity and the diffuse reflectance intensity gradually increased during tooth erosion. The LIF spectra were analyzed by curve fitting using Gaussian spectral functions to determine the true contribution of different bands in the spectra during erosion. Thus, the broad bands at 440 and 490 nm in the LIF spectra of sound enamel were resolved into four peaks centered at 409.1, 438.1, 492.4 and 523.1 nm and of sound dentin into peaks at 412.0, 440.1, 487.8 and 523.4 nm. The F410/F525 ratios derived from curve-fitted Gaussian peak amplitudes and curve areas were found to be more sensitive to erosion as compared to the diffuse reflectance ratio R500/R700 or the raw LIF spectral ratio F440/F490.
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References
Angmar-Mansson B, ten Bosch JJ (1993) Advances in methods for diagnosing coronal caries: a review. Adv Dent Res 7:70–79
Ashley PF, Blinkhorn AS, Davies RM (1998) Occlusal caries diagnosis: an in vitro histological validation of the electronic caries monitor [ECM] and other methods. J Dent 26(2):83–88
Hintze H, Wenzel A, Danielsen B, Nyvad B (1998) Reliability of visual examination, fiber-optic transillumination, bite-wing radiography and reproducibility of direct visual examination following tooth separation for the identification of cavitated carious lesions in contacting approximal surfaces. Caries Res 32(3):204–209
Ross G (1999) Caries diagnosis with the DIAGNOdent Laser: a user’s product evaluation. Ont Dent 76(2):21–24
King NM, Shaw L (1979) Value of bitewing radiographs in detection of occlusal caries. Community Dent Oral Epidemiol 7(4):218–221
Ando M, van der Veen MH, Schemehorn B, Stookey G (2001) Comparative study to quantify demineralized enamel in deciduous and permanent teeth using laser- and light-induced fluorescence techniques. Caries Res 35:464–470
De Josselin de Jong E, Sundstrom F, Westerling H, Traneus S, ten Bosch JJ (1995) A new method or in vivo quantification of changes in initial enamel caries with laser fluorescence. Caries Res 29(1):2–7
Hall AF, DeSchepper E, Ando M, Stookey GK (1997) Invitro studies of laser fluorescence for detection and quantification of mineral loss from dental caries. Adv Dent Res 11:507–514
Hibst R, Gall R (1998) Development of diode laser based fluorescence caries detector. Caries Res 32:294
Shi XQ, Welander U, Angmar-Mansson B (2000) Occlusal caries detection with KaVo DIAGNOdent and radiography: an in vitro comparison. Caries Res 34:151–158
Sheehy EC, Brailsford SR, Kidd EA, Beighton D, Zoitopoulos L (2001) Comparison between visual examination and a laser fluorescence system for in vivo diagnosis of occlusal caries. Caries Res 35:421–426
Pinelli C, Serra MC, Loffredo LCM (2002) Validity and reproducibility of a laser fluorescence system for detecting the activity of white-spot lesions on free smooth surfaces in vivo. Caries Res 36:19–24
Shi XQ, Tranaeus S, Angmar-Mansson B (2001) Comparison of QLF and DIAGNOdent for quantification of smooth surface caries. Caries Res 35:21–26
Borisova EG, Tzonko TU, Latchezar AA (2004) Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy. Lasers Surg Med 34:249–253
Lussi A, Megert B, Longbottom C, Reich E, Francescut P (2001) Clinical performance of a laser fluorescence device for detection of occlusal caries lesions. Eur J Oral Sci 109:14–19
Van der Veen MH, ten Bosch JJ (1995) Autofluorescence of bulk sound and in vitro demineralized human root dentin. Eur J Oral Sci 103:375–381
Pretty IA, Smith PW, Edgar WM, Higham SM (2003) Detection of in vitro demineralization adjacent to restorations using quantitative light induced fluorescence (QLF). Dent Mater 19:368–374
Pretty IA, Edgar WM, Higham SM (2002) Detection of in vitro demineralization of primary teeth using quantitative light-induced fluorescence (QLF). Int J Paediatr Dent 12:158–167
Uzunov TT, Borisova EG, Kamburova KP, Avramov LA (2003) Reflectance spectroscopy of human teeth in vitro. BPU-5: Fifth General conference of the Balkan Physical Union SP16-003
Subhash N, Shiny ST, Rupananda JM, Mini J (2005) Tooth caries detection by curve fitting of laser-induced fluorescence emission: a comparative evaluation with reflectance spectroscopy. Lasers Surg Med 37:320–328
Von Fraunhofer J (2004) Dissolution of dental enamel in soft drinks. Gen Dent 52(4):308–312
ten Bosch J (1996) Light scattering and related methods in caries diagnosis In: Stookey GK (ed) Early detection of dental caries. Indiana University School of Dentistry, Indianapolis, pp 81–90
Hibst R, Paulus R (1999) Caries detection by red excited fluorescence: Investigations on fluorophores. Caries Res 33:295
Hunter RS, Harold RW (1987) The measurement of appearance, 2nd edn. Wiley, New York pp 29–50
Fujimoto D, Akiba K, Nakamura N (1977) Isolation and characterization of a fluorescent material in bovine Achilles tendon collagen. Biochem Biophys Res Comm 76:1124–1129
Walters C, Eyre DR (1983) Collagen crosslinks in human dentin: Increasing content of hydroxypyridinum residues with age. Calcif Tissue Int 35:401–405
Booy M, ten Bosch JJ (1982) A fluorescent compound in bovine dental enamel matrix compared with synthetic dityrosine. Archs Oral Biol 27:417–421
Karlstrom S (1931) Physical, physiological and pathological studies of dental enamel, with special reference to the question of its vitality. A.-B Fahlcrantz’ Boktryckeri, Stockholm 182
Anderson P, Elliott JC (2000) Rates of mineral loss in human enamel during in vitro demineralization perpendicular and parallel to the natural surface. Caries Res 34:33–40
Roberson T, Heymann HO, Swift EJ (2002) Sturdevant’s Art & Science of Operative Dentistry St Louis, Missouri, Mosby
Summit, James B, William J, Robbins, Richard S, Schwartz (2001) Fundamentals of operative dentistry: A contemporary approach, 2nd edn. Quintessence Publishing, UK
Ten C (1998) Oral histology, development, structure and function, 5th edn, Mosby
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This project was carried out with support from the CESS Plan-223 and the Department of Science and Technology project grants. The authors RJM and SST acknowledge the CSIR and CESS, respectively, for their research fellowships. The authors are thankful to the Director and the Research Council of CESS for their encouragement and support.
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Thomas, S.S., Mallia, R.J., Jose, M. et al. Investigation of in vitro dental erosion by optical techniques. Lasers Med Sci 23, 319–329 (2008). https://doi.org/10.1007/s10103-007-0489-z
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DOI: https://doi.org/10.1007/s10103-007-0489-z