Lasers in Medical Science

, Volume 20, Issue 3–4, pp 138–146 | Cite as

Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix

  • Liat Abramovitch-GottlibEmail author
  • Talia Gross
  • Doron Naveh
  • Shimona Geresh
  • Salman Rosenwaks
  • Ilana Bar
  • Razi Vago
Original Article


Mesenchymal stem cells (MSCs) seeded on three-dimensional (3D) coralline (Porites lutea) biomatrices were irradiated with low-level laser irradiation (LLLI). The consequent phenotype modulation and development of MSCs towards ossified tissue was studied in this combined 3D biomatrix/LLLI system and in a control group, which was similarly grown, but was not treated by LLLI. The irradiated and non irradiated MSC were tested at 1–7, 10, 14, 21, 28 days of culturing via analysis of cellular distribution on matrices (trypan blue), calcium incorporation to newly formed tissue (alizarin red), bone nodule formation (von Kossa), fat aggregates formation (oil red O), alkaline phosphatase (ALP) activity, scanning electron microscopy (SEM) and electron dispersive spectrometry (EDS). The results obtained from the irradiated samples showed enhanced tissue formation, appearance of phosphorous peaks and calcium and phosphate incorporation to newly formed tissue. Moreover, in irradiated samples ALP activity was significantly enhanced in early stages and notably reduced in late stages of culturing. These findings of cell and tissue parameters up to 28 days of culture revealed higher ossification levels in irradiated samples compared with the control group. We suggest that both the surface properties of the 3D crystalline biomatrices and the LLLI have biostimulatory effects on the conversion of MSCs into bone-forming cells and on the induction of ex-vivo ossification.


Mesenchymal stem cells Low-level laser irradiation Osteogenesis Coral Biomaterials 



The research was partially supported by the Binational Science Foundation (BSF) # 2001045 and by the James Franck Binational German-Israeli Program in Laser-Matter Interaction. The authors are grateful to Mrs. Aviva Kiryati for expert assistance in SEM and EDS analyses. The authors wish to thank Mrs. Inez Murinek for the fruitful discussions and comments on the manuscript.


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Copyright information

© Springer-Verlag London Limited 2005

Authors and Affiliations

  • Liat Abramovitch-Gottlib
    • 1
    • 2
    Email author
  • Talia Gross
    • 1
    • 2
  • Doron Naveh
    • 3
  • Shimona Geresh
    • 1
  • Salman Rosenwaks
    • 3
  • Ilana Bar
    • 3
    • 4
  • Razi Vago
    • 1
    • 2
  1. 1.Department of Biotechnology EngineeringBen-Gurion University of the NegevBeer-ShevaIsrae
  2. 2.The National Institute of BiotechnologyBen-Gurion University of the NegevBeer-ShevaIsrael
  3. 3.Department of PhysicsBen-Gurion University of the NegevBeer-ShevaIsrael
  4. 4.The Institutes for Applied ResearchBen-Gurion University of the NegevBeer-ShevaIsrael

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