Abstract
Cultivation of microalgae in the domestic wastewater is an alternative approach over conventional activated sludge processes for simultaneous pollutants removal and biomaterials production for biorefinery. This study was aimed at growing a Chlorella variabilis TH03 in domestic wastewater using various closed photobioreactors and open-reactor systems for evaluation of the algal growth, pollutants removal efficiency, biomass productivity and characterization of the biomass. Results indicated that the C. variabilis TH03 exhibited an outstanding growth, achieving maximum biomass concentration of 1.52, 1.67, 1.54 and 1.72 g/L, with specific growth rate of 0.32/day, 0.27/day, 0.22/day and 0.34/day in PBR-5-R, PBR-5-S, T-50 and RW-500 reactor systems, respectively. Remarkably, additional supplement of CO2 significantly enhanced areal biomass productivity of C. variabilis TH03 grown in RW-500 from 13.1 to 38.5 g/m2 day. Pollutants in term of COD, total nitrogen and total phosphorous were removed by 74.8–89.8%, 93.8–96.1% and 97.1–99.9%, respectively, during 14–17 days cultivation of the C. variabilis TH03. The fatty acid methyl esters of the algal biomass was determined as 22.55–26.12% with the most abundant fatty acids of C16:0 (26.13–27.67%), C16:3n4 (20.07–22.24%) and C18:3n3 (34.83–36.79%) which were desirable fatty acids for biodiesel and bio-jet fuels synthesis. Moreover, total carbohydrates and proteins in the algal biomass were accumulated up to 31.56–35.23% and 33.67–37.34%, respectively. Our preliminary results demonstrated the cultivation technology of C. variabilis TH03 can sustainably replace the conventional activated sludge technologies for domestic wastewater treatment.
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Alam MA, Wu J, Xu J, Wang Z (2019a) Enhanced isolation of lipids from microalgal biomass with high water content for biodiesel production. Bioresour Technol 291:121834
Alam MA, Yuan T, Xiong W, Zhang B, Lv Y, Xu J (2019b) Process optimization for the production of high-concentration ethanol with Scenedesmus raciborskii biomass. Bioresour Technol 294:122219
ARCOWA (2018) Wastewater management and resource recovery in Vietnam: current status and opportunities
Berges JA, Fisher AE, Harrison PJ (1993) A comparison of Lowry, Bradford and Smith protein assays using different protein standards and protein isolated from the marine diatom Thalassiosira pseudonana. Mar Biol 115:187–193
Cai T, Park SY, Li Y (2013) Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew Sust Energy Rev 19:360–369
Caporgno MP, Mathys A (2018) Trends in microalgae incorporation into innovative food products with potential health benefits. Front Nutr 5:1–10
Chamberlin J, Harrison K, Zhang W (2018) Impact of nutrient availability on tertiary wastewater treatment by Chlorella vulgaris. Water Environ Res 90:2008–2016
Cheah WY, Show PL, Juan JC, Chang JS, Ling TC (2018) Waste to energy: the effects of Pseudomonas sp. on Chlorella sorokiniana biomass and lipid productions in palm oil mill effluent. Clean Technol Environ Policy 20:2037–2045
Chen CY, Zhao XQ, Yen HW et al (2013) Microalgae-based carbohydrates for biofuel production. Biochem Eng J 78:1–10
Chi Z, Zheng Y, Jiang A, Chen S (2011) Lipid production by culturing oleaginous yeast and algae with food waste and municipal wastewater in an integrated process. Appl Biochem Biotechnol 165:442–453
Chinnasamy S, Bhatnagar A, Hunt RW, Das KC (2010) Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresour Technol 101:3097–3105
Choi HJ, Lee SM (2015) Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater. Bioprocess Biosyst Eng 38:761–766
Chokshi K, Pancha I, Ghosh A, Mishra S (2017) Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus. Biotechnol Biofuels 10:60
Collos Y, Berges J (2009) Nitrogen metabolism in phytoplankton. In: Duarte CM, Helgueras AL (eds) Marine ecology. Encyclopedia of Life Support Systems (EOLSS)
Conley DJ, Paerl HW, Howarth RW et al (2009) Controlling eutrophication: nitrogen and phosphorus. Science 323:1014–1015
Craggs R, Sutherland D, Campbell H (2012) Hectare-scale demonstration of high rate algal ponds for enhanced wastewater treatment and biofuel production. J Appl Phycol 24:329–337
Dalrymple OK, Halfhide T, Udom I, Gilles B, Wolan J, Zhang Q, Ergas S (2013) Wastewater use in algae production for generation of renewable resources: a review and preliminary results. Aquat Biosyst 9:2
de-Bashan LE, Trejo A, Huss VAR, Hernandez JP, Bashan Y (2008) Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater. Bioresour Technol 99:4980–4989
Dong T, Van Wychen S, Nagle N, Pienkos PT, Laurens LML (2016) Impact of biochemical composition on susceptibility of algal biomass to acid-catalyzed pretreatment for sugar and lipid recovery. Algal Res 18:69–77
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Gao F, Yang HL, Li C et al (2019) Effect of organic carbon to nitrogen ratio in wastewater on growth, nutrient uptake and lipid accumulation of a mixotrophic microalgae Chlorella sp. Bioresour Technol 282:118–124
Gerardi MH (2003) Introduction to nitrification. In: Nitrification and denitrification in the activated sludge process, Wiley, pp 35–41
Ho SH, Huang SW, Chen CY, Hasunuma T, Kondo A, Chang JS (2013) Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E. Bioresour Technol 135:157–165
Hu B, Min M, Zhou W et al (2012) Enhanced mixotrophic growth of microalga Chlorella sp. on pretreated swine manure for simultaneous biofuel feedstock production and nutrient removal. Bioresour Technol 126:71–79
Kabir F, Gulfraz M, Raja GK et al (2018) Nutrients utilization and biomass production by microalgae culture development in wastewater. Int J Biosci 12:460–469
Kamyab H, Din MFM, Hosseini SE et al (2016) Optimum lipid production using agro-industrial wastewater treated microalgae as biofuel substrate. Clean Technol Environ Policy 18:2513–2523
Larsdotter K (2006) Wastewater treatment with microalgae—a literature review. Vatten 62:31–38
Laurens LML, Dempster TA, Jones HDT et al (2012) Algal biomass constituent analysis: method uncertainties and investigation of the underlying measuring chemistries. Anal Chem 84:1879–1887
Laurens LM, Van Wychen S, McAllister JP, Arrowsmith S, Dempster TA, McGowen J, Pienkos PT (2014) Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition. Anal Biochem 452:86–95
Le TG, Tran DT, Van Do TC, Nguyen VT (2019) Design considerations of microalgal culture ponds and photobioreactors for wastewater treatment and biomass cogeneration. In: Alam MA, Wang Z (eds) Microalgae biotechnology for development of biofuel and wastewater treatment. Springer, Singapore, pp 535–567
Leong WH, Lim JW, Lam MK, Uemura Y, Ho CD, Ho YC (2018) Co-cultivation of activated sludge and microalgae for the simultaneous enhancements of nitrogen-rich wastewater bioremediation and lipid production. J Taiwan Inst Chem Eng 87:216–224
Li Y, Chen YF, Chen P et al (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102:5138–5144
Liang K, Zhang Q, Gu M, Cong W (2013) Effect of phosphorus on lipid accumulation in freshwater microalga Chlorella sp. J Appl Phycol 25:311–318
Ma M, Wei C, Wang H, Sha C, Chen M, Gong Y, Hu Q (2019) Isolation and evaluation of a novel strain of Chlorella sorokiniana that resists grazing by the predator Poterioochromonas malhamensis. Algal Res 38:101429
Matos ÂP (2019) Chapter 3—microalgae as a potential source of proteins. In: Galanakis CM (ed) Proteins: sustainable source, processing and applications. Academic Press, Cambridge, pp 63–96
Matusiak K (1976) Studies on the purification of wastewater from the nitrogen fertilizer industry by intensive algal cultures. I. Growth of Chlorella vulgaris in wastes. Acta Microbiol Pol 25:233–242
Michelon W, Da Silva MLB, Mezzari MP, Pirolli M, Prandini JM, Soares HM (2016) Effects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate. Appl Biochem Biotechnol 178:1407–1419
Ogawa T, Aiba S (1981) Bioenergetic analysis of mixotrophic growth in Chlorella vulgaris and Scenedesmus acutus. Biotechnol Bioeng 23:1121–1132
Oswald WJ (1988) Micro-algae and waste-water treatment. Cambridge University Press, Cambridge
Pagnanelli F, Altimari P, Trabucco F, Toro L (2014) Mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata: interaction between glucose and nitrate. J Chem Technol Biotechnol 89:652–661
Park JBK, Craggs RJ, Shilton AN (2011) Wastewater treatment high rate algal ponds for biofuel production. Bioresour Technol 102:35–42
Petkov G, Garcia G (2007) Which are fatty acids of the green alga Chlorella? Biochem Syst Ecol 35:281–285
Pham TM, Doan BH, Tran DT, Nguyen TH, Pham TMH, Nguyen QT (2019) Study on the harvesting methods of Chlorella sorokiniana and Scenedesmus acuminatus cultured in minicipal wastewater. J Sci Technol 52:70–85
Pienkos PT, Darzins A (2009) The promise and challenges of microalgal-derived biofuels. Biofuels, Bioprod Biorefin 3:431–440
Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–25
Pushpakumari Kudahettige N, Pickova J, Gentili FG (2018) Stressing algae for biofuel production: biomass and biochemical composition of Scenedesmus dimorphus and Selenastrum minutum grown in municipal untreated wastewater. Front Energy Res 6:1–10
QCVN 14:2008/BTNM. National technical regulation on domestic wastewater
Rawat I, Ranjith Kumar R, Mutanda T, Bux F (2011) Dual role of microalgae: phycoremediation of domestic wastewater and biomass production for sustainable biofuels production. Appl Energy 88:3411–3424
Reeves TG (1972) Nitrogen removal: a literature review. Journal (Water Pollut Control Fed) 44:1895–1908
Rice EW, Baird RB, Eaton AD (2017) Standard methods for the examination of water and wastewater, 23rd edn. American Public Health Association, American Water Works Association, Water Environment Federation
Said HA (2009) Changes in levels of cellular constituents of Dunaliella parva associated with inorganic phosphate depletion. Middle East J Sci Res 4:44–49
Song Y, Hahn HH, Hoffmann E (2002) Effects of solution conditions on the precipitation of phosphate for recovery: a thermodynamic evaluation. Chemosphere 48:1029–1034
Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87–96
Tam NFY, Wong YS (1989) Wastewater nutrient removal by Chlorella pyrenoidosa and Scenedesmus sp. Environ Pollut 58:19–34
Vo PL (2007) Urbanization and water management in Ho Chi Minh City, Vietnam-issues, challenges and perspectives. GeoJournal 70:75–89
Woertz I, Feffer A, Lundquist T, Nelson Y (2009) Algae grown on dairy and municipal wastewater for simultaneous nutrient removal and lipid production for biofuel feedstock. J Environ Eng 135:1115–1122
Xu H, Miao X, Wu Q (2006) High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 126:499–507
Zhao Y, Yu Z, Song X, Cao X (2009) Biochemical compositions of two dominant bloom- forming species isolated from the Yangtze River Estuary in response to different nutrient conditions. J Exp Mar Biol Ecol 368:30–36
Zhou W, Li Y, Min M et al (2012) Growing wastewater-born microalga Auxenochlorella protothecoides UMN280 on concentrated municipal wastewater for simultaneous nutrient removal and energy feedstock production. Appl Energy 98:433–440
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This research is funded by Graduate University of Science and Technology under the Grant Number GUST.STS.ĐT2017-ST03.
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Tran, D., Van Do, T.C., Nguyen, Q.T. et al. Simultaneous removal of pollutants and high value biomaterials production by Chlorella variabilis TH03 from domestic wastewater. Clean Techn Environ Policy 23, 3–17 (2021). https://doi.org/10.1007/s10098-020-01810-5
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DOI: https://doi.org/10.1007/s10098-020-01810-5