Skip to main content

Advertisement

SpringerLink
Log in

Activity enhancement of carbonic anhydrase in Chlamydomonas sp. for effective CO2 sequestration

  • Original Paper
  • Published:
Clean Technologies and Environmental Policy Aims and scope Submit manuscript

Abstract

Present study describes the induction of carbonic anhydrase (CA) activity in Chlamydomonas sp. in presence of calcium carbonate. Results indicated that calcium carbonate increased the activity of extracellular CA enzyme by 14-fold compared to culture grown at 3 % CO2, and by fourfold compared to the culture grown at 0.03 % CO2. CA was purified by gel exclusion followed by ion exchange chromatography. Molecular weight of the purified CA was found to be approximately 28 kDa. The enzyme showed optimum stability in the pH range of 8.0–9.0 and temperature of 35 °C. CA activity was inhibited with Hg2+ and Pb2+, while Zn2+ was found to accelerate its activity. Purified CA was employed for carbonation reaction with CO2 and the calcite produced was characterized by scanning electron microscope and X-ray diffraction. The enhancement in CA activity was found to be reliable, convenient, and may be employed for CO2 sequestration using whole cells or immobilized bioreactor systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Baba M, Suzuki I, Shiraiwa Y (2011) Proteomic analysis of high-CO2-inducible extracellular proteins in the unicellular green alga, Chlamydomonas reinhardtii. Plant Cell Physiol 52:1302–1314

    Article  CAS  Google Scholar 

  • Bandyopadhyay A (2011) Amine versus ammonia absorption of CO2 as a measure of reducing GHG emission: a critical analysis. Clean Technol Environ Policy 13:269–294

    Article  CAS  Google Scholar 

  • Farve N, Christ ML, Pierre AC (2009) Biocatalytic capture of CO2 with carbonic anhydrase and its transformation to solid carbonate. J Mol Catal B Enzym 60:163–170

    Article  Google Scholar 

  • Figueroa JD, Fout T, Plasynski S, McIlvried H, Srivastava RD (2008) Advances in CO2 capture technology—The U.S. Department of Energy’s Carbon Sequestration Program. Int J Greenh Gas Control 2:9–20

    Article  CAS  Google Scholar 

  • Jansson C, Northen T (2010) Calcifying cyanobacteria—the potential of biomineralization for carbon capture and storage. Curr Opin Biotechnol 736:1–7

    Google Scholar 

  • Karlsson J, Hiltonen T, Husic HD, Ramazanov Z, Samuelsson C (1995) lntracellular carbonic anhydrase of Chlamydomonas reinhardtii. Plant Physiol 109:533–539

    Article  CAS  Google Scholar 

  • Klemes JJ, Varbanov PS (2012) Efficient and clean production of fuels and biofuels: a summary. Clean Technol Environ Policy 14:371–375

    Article  Google Scholar 

  • Krungkrai SR, Suraveratumb N, Rochanakijc S, Krungkrai J (2001) Characterization of carbonic anhydrase in Plasmodium falciparum. Int J Parasitol 31:661–668

    Article  CAS  Google Scholar 

  • Kupriyanova E, Villarejo A, Markelova A, Gerasimenko L, Zavarzin G, Samuelsson G, Los DA, Pronina N (2007) Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes. Microbiology 153:1149–1156

    Article  CAS  Google Scholar 

  • Maroušek J (2013a) Removal of hardly fermentable ballast from the maize silage to accelerate biogas production. Ind Crop Prod 44:253–257

    Article  Google Scholar 

  • Maroušek J (2013b) Two-fraction anaerobic fermentation of grass waste. J Sci Food Agric 93:2410–2414

    Article  Google Scholar 

  • Moroney JV, Ynalvez RA (2007) Proposed carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii. Eukaryot Cell 6:1251–1259

    Article  CAS  Google Scholar 

  • Moroney JV, Bartlett SG, Samuelsson G (2001) Carbonic anhydrase in plants and algae. Plant Cell Environ 24:141–153

    Article  CAS  Google Scholar 

  • Premkumar L, Bageshwar UK, Gokhman I, Zamir A, Sussman JL (2003) An unusual halotolerant α-type carbonic anhydrase from the algae Dunaliella salina functionally expressed in E. coli. Protein Expr Purif 28:151–157

    Article  CAS  Google Scholar 

  • Ramanan R, Kannan K, Devi SS, Mudliar S, Kaur S, Tripathi AK, Chakrabarti T (2009a) Bio-sequestration of carbon dioxide using carbonic anhydrase enzyme purified from Citrobacter freundii. World J Microbiol Biotechnol 25:981–987

    Article  CAS  Google Scholar 

  • Ramanan R, Krishnamurthi K, Vinayagamoorthy N, Ramkumar KM, Devi SS, Chakrabarti T (2009b) Purification and characterization of a novel plant-type carbonic anhydrase from Bacillus subtilis. Biotechnol Bioproc Eng 14:32–37

    Article  CAS  Google Scholar 

  • Ramanan R, Krishnamurthi K, Deshkar A, Yadav R, Chakarbarti T (2010) Enhanced algal CO2 sequestration through calcite deposition by Chlorella sp. and Spirulina platensis in a mini-raceway pond. Bioresour Technol 101:2616–2622

    Article  CAS  Google Scholar 

  • Roberts SB (1997) Carbonic anhydrase in the marine diatom Thalassiosira weissflogii (bacillarlophyceae). J Phycol 33:845–850

    Article  CAS  Google Scholar 

  • Sharma A, Bhattacharya A, Singh S (2009) Purification and characterization of an extracellular carbonic anhydrase from Pseudomonas fragi. Process Biochem 44:1293–1297

    Article  CAS  Google Scholar 

  • Shekh AY, Krishnamurthi K, Mudliar SN, Yadav RR, Fulke AB, Devi SS, Chakrabarti T (2012) Recent advancements in carbonic anhydrase driven processes for CO2 sequestration: minireview. Crit Rev Environ Sci Technol 42:1419–1440

    Article  CAS  Google Scholar 

  • Spijkerman E (2011) The expression of a carbon concentrating mechanism in Chlamydomonas acidophila under variable phosphorus, iron, and CO2 concentrations. Photosynth Res 109:179–189

    Article  CAS  Google Scholar 

  • Turkmen H, Durgun M, Yilmaztekin S, Emul M, Innocenyi A, Vullo D, Scozzafava A, Supuran CT (2005) Carbonic anhydrase inhibitors. Novel sulfanilamide/acetazolamide derivatives obtained by the tail approach and their interaction with the cytosolic isozymes I and II, and the tumor-associated isozyme IX. Bioorg Med Chem Lett 17:367–372

    Article  Google Scholar 

  • Wilbur KM, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 176:147–154

    CAS  Google Scholar 

  • Yadav RR, Mudliar SN, Shekh AY, Fulke AB, Devi SS, Krishnamurthi K, Juwarkar A, Chakrabarti T (2012) Immobilization of carbonic anhydrase in alginate and its influence on transformation of CO2 to calcite. Process Biochem 47:585–590

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Financial support provided by Department of Biotechnology (DBT) and Department of Science & Technology (DST), Government of India is gratefully acknowledged. The authors are grateful to Dr. S. R. Wate, Director CSIR-NEERI, for his encouragement and time-to-time constructive suggestions.

Author information

Authors and Affiliations

  1. Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, India

    Raju R. Yadav, Kannan Krishnamurthi, Ajam Y. Shekh, Saravana S. Devi & Tapan Chakrabarti

  2. Environmental Biotechnology Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, India

    Sandeep N. Mudliar

Authors
  1. Raju R. Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kannan Krishnamurthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ajam Y. Shekh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sandeep N. Mudliar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Saravana S. Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tapan Chakrabarti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kannan Krishnamurthi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yadav, R.R., Krishnamurthi, K., Shekh, A.Y. et al. Activity enhancement of carbonic anhydrase in Chlamydomonas sp. for effective CO2 sequestration. Clean Techn Environ Policy 16, 1827–1833 (2014). https://doi.org/10.1007/s10098-014-0734-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10098-014-0734-7

Keywords

Search

Navigation