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Evaluation of the E Test for Antifungal Susceptibility Testing of Candida glabrata

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Abstract

 The E test was compared to the reference NCCLS broth macrodilution method for susceptibility testing of Candida (Torulopsis) glabrata. The MICs of amphotericin B, flucytosine, fluconazole and itraconazole were determined using the appropriate culture media (RPMI 1640 agar with 2% glucose, Casitone agar or Antibiotic Medium 3 agar) according to the drug tested. Agreement between the two methods was within plus/minus two dilutions for 77–100% of test results, according to the drug/medium combination. The study revealed problems in determining the MICs of azoles using the E test, and confirmed the suitability of Casitone agar for susceptibility testing of fluconazole even if results were read within 24 h.

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Authors and Affiliations

  1. Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire (EA 864), Faculté de Pharmacie, 27 boulevard Jean Moulin, 13385 Marseille Cedex 5, France e-mail: Reglip@pharmacie.univ-mrs.fr, , , , , , FR

    A. Favel, C. Chastin, A. L. Thomet & P. Regli

  2. Laboratoire de Bactériologie et Parasitologie, CHU Nord, chemin Bourrely, 13015 Marseille Cedex, France, , , , , , FR

    A. Michel-Nguyen & A. Penaud

Authors
  1. A. Favel
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  2. C. Chastin
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  3. A. L. Thomet
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  4. P. Regli
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  5. A. Michel-Nguyen
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  6. A. Penaud
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Favel, A., Chastin, C., Thomet, A. et al. Evaluation of the E Test for Antifungal Susceptibility Testing of Candida glabrata . EJCMID 19, 146–148 (2000). https://doi.org/10.1007/s100960050448

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  • DOI: https://doi.org/10.1007/s100960050448

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