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Evaluation of a New Commercial Immunoassay >for Rapid Detection of Campylobacter jejuni in Stool Samples

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European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

 In order to evaluate a new commercial enzyme immunoassay (ProspecT Campylobacter Microplate Assay; Alexon-Trend, USA) for the detection of Campylobacter jejuni and Campylobacter coli in stool samples, 30 faecal specimens known to be culture-positive for Campylobacter jejuni were tested with the new assay. The detection limit was approximately 3×106/ml in faecal suspensions. The sensitivity relative to culture was 80% (24/30). All of the 24 positive samples, except for one, remained positive after being stored at –20  °C for 60 days. The specificity of the test was 100%. Interestingly, 6 of 11 additional Campylobacter jejuni culture-positive samples that had been obtained from patients with Guillain-Barré syndrome and stored at –20  °C for periods of up to 5 years tested positive in the assay. The performance of the assay indicates that it has potential value for use in future early intervention studies.

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Endtz, H., Ang, C., van den Braak, N. et al. Evaluation of a New Commercial Immunoassay >for Rapid Detection of Campylobacter jejuni in Stool Samples. EJCMID 19, 794–797 (2000). https://doi.org/10.1007/s100960000368

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  • DOI: https://doi.org/10.1007/s100960000368

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