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Characterization of an outbreak caused by Elizabethkingia miricola using Fourier-transform infrared (FTIR) spectroscopy

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Abstract

Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020–2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is useful for E. miricola typing and can be easily implemented in the laboratory.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the projects PI18/00997 from the Health Research Fund (FIS. Instituto de Salud Carlos III. Plan Nacional de I+D+I 2013-2016) of the Carlos III Health Institute (ISCIII, Madrid, Spain) partially financed by the European Regional Development Fund (FEDER) “A way of making Europe.” BRS (CPII19/00002) is the recipient of a Miguel Servet contract supported by the FIS. DRT has been funded by the IiSGM through its intramural programme.

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Authors and Affiliations

  1. Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo, 46, 28007, Madrid, Spain

    David Rodríguez-Temporal, Ana Candela, Julia Serrano-Lobo, Belén Rodríguez-Sánchez & Emilia Cercenado

  2. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

    David Rodríguez-Temporal, Ana Candela, Belén Rodríguez-Sánchez & Emilia Cercenado

  3. Laboratorio de Referencia e Investigación en Resistencia a Antibióticos, Centro Nacional de Microbiología, ISCIII, Majadahonda, Spain

    Javier Enrique García-Cañada, Jesús Oteo-Iglesias & María Pérez-Vázquez

  4. CIBER de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain

    Javier Enrique García-Cañada, Jesús Oteo-Iglesias & María Pérez-Vázquez

  5. CIBER de Enfermedades Respiratorias (CIBERES), Madrid, Spain

    Emilia Cercenado

Authors
  1. David Rodríguez-Temporal
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  2. Javier Enrique García-Cañada
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  3. Ana Candela
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  4. Jesús Oteo-Iglesias
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  7. Belén Rodríguez-Sánchez
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  8. Emilia Cercenado
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Contributions

DRT: conceptualization, experimentation, formal analysis, data collection, validation, visualization, original draft preparation, and review/editing. AC: experimentation, formal analysis, validation, writing and review/editing. JEGC, JOI, MPV, JS: experimentation, data analysis, writing and review/editing. BRS, EC: conceptualization, experimentation, project administration, formal analysis, supervision, validation, visualization, original draft preparation and review/editing.

Corresponding author

Correspondence to David Rodríguez-Temporal.

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The Ethics Committee of the Gregorio Marañón Hospital (CEIm) evaluated this project and considered that all the conditions for waiving informed consent were met, since the study was conducted with microbiological samples and not with human products.

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The authors declare no competing interests.

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Rodríguez-Temporal, D., García-Cañada, J.E., Candela, A. et al. Characterization of an outbreak caused by Elizabethkingia miricola using Fourier-transform infrared (FTIR) spectroscopy. Eur J Clin Microbiol Infect Dis 43, 797–803 (2024). https://doi.org/10.1007/s10096-024-04764-4

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