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Investigation of the presence of Capnophilic bacteria in routine urine cultures

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Abstract

Capnophilic Escherichia coli (CEC) strains are rarely isolated from urinary tract infections (UTIs). The purpose of this research was to look into the incidence and traits of the CEC strains that cause UTIs. Nine (0.11%) epidemiologically unrelated CEC isolates with varying antibiotic susceptibility patterns were identified from patients with various co-morbidities after the evaluation of 8500 urine samples. Three of these strains belonged to the O25b-ST131 clone, and none of them possessed the yadF gene. Due to adverse incubation conditions, CEC isolation is difficult. Although rare, capnophilic incubation of urine cultures may be considered particularly for patients with underlying predisposing conditions.

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Ankara University School of Medicine, Ankara, Turkey

    Zeynep Ceren Karahan, Ebru Evren, Selin Gamze Kiliç, İştar Dolapçi & Alper Tekeli

  2. Central Microbiology Laboratory, Ibn-I Sina Hospital, Ankara University School of Medicine, Ankara, Turkey

    Zeynep Ceren Karahan & Ebru Evren

  3. Ankara University School of Medicine, Ankara, Turkey

    İrem Altinsoy, Bilge Nur Çalişkan, Sıla Dede, Görkem Kayiş & Hasan Can Türkoğlu

  4. Department of Biostatistics, Ankara University School of Medicine, Ankara, Turkey

    Beyza Doğanay Erdoğan

Authors
  1. Zeynep Ceren Karahan
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and phenotypic analysis (including culture and antimicrobial susceptibility testing) of the capnophilic strains were performed by Zeynep Ceren Karahan, İrem Altınsoy, Bilge Nur Çalışkan, Sıla Dede, Görkem Kayış, Hasan Can Türkoğlu, and Ebru Evren. yadF PCR analysis was performed by Zeynep Ceren Karahan. Determination of the phylogenetic group and investigation of the O25b-ST131 clone by PCR analysis was performed by Selin Gamze Kılıç and İştar Dolapçı. PFGE analysis was performed by Alper Tekeli. Statistical analysis was performed by Beyza Doğanay Erdoğan. The first draft of the manuscript was written by Zeynep Ceren Karahan, and all authors commented and contributed to the previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zeynep Ceren Karahan.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the “Undergraduate Student Researches Ethics Committee” of Ankara University School of Medicine (24.12.2018/9492).

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The authors declare no competing interests.

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Karahan, Z.C., Altinsoy, İ., Çalişkan, B.N. et al. Investigation of the presence of Capnophilic bacteria in routine urine cultures. Eur J Clin Microbiol Infect Dis 42, 519–524 (2023). https://doi.org/10.1007/s10096-023-04570-4

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  • DOI: https://doi.org/10.1007/s10096-023-04570-4

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