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Antibiotic resistance and resistance mechanism of Corynebacterium kroppenstedtii isolated from patients with mastadenitis

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Abstract

To investigate the antibiotic resistance and resistance mechanism of Corynebacterium kroppenstedtii (C. kroppenstedtii) isolated from patients with mastadenitis. Ninety C. kroppenstedtii clinical isolates were obtained from clinical specimens in 2018–2019. Species identification was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed by the broth microdilution method. The resistance genes were detected using PCR and DNA sequencing. The results of antimicrobial susceptibility testing indicated that the resistance rates of C. kroppenstedtii to erythromycin and clindamycin, ciprofloxacin, tetracycline, and trimethoprim-sulfamethoxazole were 88.9%, 88.9%, 67.8%, 62.2%, and 46.6%, respectively. None of the C. kroppenstedtii isolates was resistant to rifampicin, linezolid, vancomycin, or gentamicin. The gene of erm(X) was detected in all clindamycin and erythromycin-resistant strains. The gene of sul(1) and tet(W) were detected among all trimethoprim sulfamethoxazole-resistant strains and tetracycline-resistant strains, respectively. Furthermore, 1 or 2 amino acid mutations (mainly single mutation) were observed in the gyrA gene among ciprofloxacin-resistant strains.

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Data availability

The data analyzed were extracted from our hospital’s lab computer and the computerized patient records. The analysis was done transparently, and the database is available.

Materials availability

The data analyzed were extracted from our hospital’s lab computer and the computerized patient records. The analysis was done transparently, and the database is available.

Code availability

Not applicable.

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Funding

This work was funded by the National Key Research and Development Program of China (2021YFC2701800 and 2021YFC2701803), the National Natural Science Foundation of China (grant no. 81861138052), the China Antimicrobial Surveillance Network (independent medical grants from Pfizer, 2018QD100), and the Shanghai Antimicrobial Surveillance Network (3030231003). The funders had no role in study design, data collection and analysis, decision to publish, or manuscript preparation.

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Author notes

  1. Qin Zhang and Shi Wu equally contributed to this study and are co-first authors.

Authors and Affiliations

  1. Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China

    Qin Zhang, Peipei Song, Yi Liu, Xuan Zhu, Liyuan Zhang, Jun Zhang, Qin Wang & Jian Xu

  2. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China

    Qin Zhang, Shi Wu, Li Ding, Qingyu Shi & Fupin Hu

Authors
  1. Qin Zhang
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  2. Shi Wu
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  3. Peipei Song
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  4. Yi Liu
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  5. Li Ding
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  6. Qingyu Shi
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  7. Xuan Zhu
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  8. Liyuan Zhang
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  9. Jun Zhang
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  10. Qin Wang
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  11. Jian Xu
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  12. Fupin Hu
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Contributions

The study’s conception and design were prepared by Qin Zhang, Jian Xu, and Fupin Hu. Materials were prepared by Qin Zhang, Peipei Song, Yi Liu, Xuan Zhu, Liyuan Zhang, Jun Zhang, Qin Wang, and Jian Xu. Experimentation, data collection, and analysis were performed by Qin Zhang, Shi Wu, Li Ding, and Qingyu Shi. The first draft of the manuscript was written by Qin Zhang, Jian Xu, and Fupin Hu, and the manuscript was reviewed and edited by Fupin Hu. All the authors approved the final manuscript.

Corresponding authors

Correspondence to Jian Xu or Fupin Hu.

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Ethics approval

This study was reviewed and approved by the Ethics Committee of Chengdu Women’s and Children’s Central Hospital. Informed consent from patients was not required by the Ethics Committee because there was no contact with patients.

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Informed consent from patients was not required by the Ethics Committee.

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Not applicable.

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The authors declare no competing interests.

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Zhang, Q., Wu, S., Song, P. et al. Antibiotic resistance and resistance mechanism of Corynebacterium kroppenstedtii isolated from patients with mastadenitis. Eur J Clin Microbiol Infect Dis 42, 525–528 (2023). https://doi.org/10.1007/s10096-023-04558-0

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  • DOI: https://doi.org/10.1007/s10096-023-04558-0

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