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Direct next-generation sequencing diagnosis of echovirus 9 meningitis, France

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Abstract

The prognosis of central nervous system infections caused by enteroviruses partially depends on the viral genotype, which is not provided by current point-of-care diagnostic methods. In this study, next-generation sequencing identified an echovirus 9 directly from the cerebrospinal fluid of a patient presenting with meningitis.

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Data Availability

GenBank accession nos. LR815569.1, LR815570.1, LR815571.1, LR815572.1, LR815573.1.

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Funding

This work was supported by the French Government under the Investissements d’Avenir (Investments in the Future) programme managed by the Agence Nationale de la Recherche (ANR,fr: National Agency for Research) [reference: Méditerranée Infection 10-IAHU-03]. This work was also supported by Région Le Sud (Provence-Alpes-Côte d’Azur) and European funding [FEDER PA 0000320 PRIMMI]. MORSLI Madjid receives a PhD grant from Fondation Méditerranée infection, Marseille, France.

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Authors and Affiliations

  1. IHU Méditerranée Infection, Marseille, France

    Madjid Morsli, Jean-Jacques Vincent, Laurine Milliere, Philippe Colson & Michel Drancourt

  2. Aix-Marseille Université, IRD, IHU Méditerranée Infection, Marseille, France

    Madjid Morsli, Philippe Colson & Michel Drancourt

  3. Laboratoire de Microbiologie, Assistance Publique-Hôpitaux de Marseille, IHU Méditerranée Infection, Marseille, France

    Jean-Jacques Vincent, Laurine Milliere & Michel Drancourt

Authors
  1. Madjid Morsli
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  2. Jean-Jacques Vincent
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  3. Laurine Milliere
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  4. Philippe Colson
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  5. Michel Drancourt
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Contributions

MM contributed to experimental design, realization of the work, data analysis, interpretation, and writing. JJV and LM collected samples and clinical information. PC and MD contributed to critically reviewing the manuscript and data interpretation. MD coordinated and directed the work.

Corresponding author

Correspondence to Michel Drancourt.

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Ethical Approval

All data were generated as part of routine work at the Assistance Publique-Hôpitaux de Marseille (Marseille University hospitals), and this study is the result of routine clinical management. No specific clinical sampling has been done in this study.

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Not applicable.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Morsli, M., Vincent, JJ., Milliere, L. et al. Direct next-generation sequencing diagnosis of echovirus 9 meningitis, France. Eur J Clin Microbiol Infect Dis 40, 2037–2039 (2021). https://doi.org/10.1007/s10096-021-04205-6

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  • DOI: https://doi.org/10.1007/s10096-021-04205-6

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