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Frequent detection of enterovirus D68 and rhinovirus type C in children with acute respiratory infections

European Journal of Clinical Microbiology & Infectious Diseases volume 40, pages 637–642 (2021)Cite this article

Abstract

This study aimed to evaluate the prevalence of human rhinoviruses (HRVs) and the emergence of enterovirus D68 (EV-D68) in children. A total of 322 nasopharyngeal swab samples were provided from children with an initial diagnosis of upper and lower respiratory tract infections. A total of 34 and 70 cases were positive for EV-D68 and HRV, respectively. The phylogenetic analysis revealed that the clades A and B are the prevalent genotypes for EV-D68 and the HRV-positive samples belong to three types including HRV-A, HRV-B, and HRV-C. The results showed that EV-D68 and HRV-C are circulating in Iran especially in the winter.

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Data availability

All data generated or analyzed during this study are included in this published article. In addition, all sequences obtained in this study have been submitted to NCBI’s GenBank sequence database and accession numbers MH250142-MH250148 and MH356731-MH356744 were assigned for partial VP1 gene sequences of EV-D68 and 5′-UTR sequences of HRV, respectively.

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Funding

This work was financially supported by Research Center of Tehran University of Medical Sciences (grant no. 31864).

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Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

    Sayed-Hamidreza Mozhgani

  2. Non-communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran

    Sayed-Hamidreza Mozhgani

  3. The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

    Mohsen Keshavarz

  4. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 1471613151, Iran

    Neda Mousavi, Vahid Salimi, Talat Mokhtari-Azad, Nastaran Ghavami & Farhad Rezaei

  5. Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Haideh Namdari

  6. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Mohadeseh Zarei-Ghobadi

  7. Virology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Seyed Alireza Nadji

Authors
  1. Sayed-Hamidreza Mozhgani
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  2. Mohsen Keshavarz
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Contributions

SHM, MZGh, MK, and FR performed bioinformatics and statistical analysis. SHM, MZGh, and FR interpreted and wrote the manuscript. FR, NM, HN, VS, TMA, SAN, and NGh performed experiments. NM contributed with patient samples. FR obtained study funding. FR and TMA supervised the study. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Farhad Rezaei.

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The authors declare that they have no competing interests.

Ethics approval and consent to participate

This study was approved by ethical committee of Tehran University of Medical Sciences.

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Mozhgani, SH., Keshavarz, M., Mousavi, N. et al. Frequent detection of enterovirus D68 and rhinovirus type C in children with acute respiratory infections. Eur J Clin Microbiol Infect Dis 40, 637–642 (2021). https://doi.org/10.1007/s10096-020-04051-y

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