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Rapid identification of fungi from respiratory samples by Bruker Biotyper matrix–assisted laser desorption/ionisation time-of-flight using ID-FUNGI plates

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Abstract

Identification of moulds is crucial for the clinical management of patients. The goal of this study was to evaluate the new ID-FUNGI plate (IDFP) for the identification of moulds by MALDI Biotyper. IDFP was compared with Sabouraud with gentamicin and chloramphenicol plate (SAB) for the identification of 80 moulds from respiratory samples and eight reference strains. With the direct transfer method, species identification rose from 6% with SAB to 68% with IDFP using score cut-off 2 and from 20 to 75% using cut-off 1.7 (p < 0.001). Our study highlights that the new IDFP improves mycological diagnostic and workflow in laboratories.

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Authors and Affiliations

  1. Department of Microbiology, Foch Hospital, 92150, Suresnes, France

    Emilie Cardot Martin, Claudine Renaux, Lucie Limousin & Marc Vasse

  2. Department of Pneumonology, Foch Hospital, 92150, Suresnes, France

    Emilie Catherinot & Louis Jean Couderc

  3. UPRES EA 220, Department of Pneumonology, Faculty of Life Sciences Simone Veil, University Paris-Saclay, 91190, Saint-Aubin, France

    Louis Jean Couderc

Authors
  1. Emilie Cardot Martin
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  2. Claudine Renaux
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  3. Emilie Catherinot
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  4. Lucie Limousin
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  5. Louis Jean Couderc
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  6. Marc Vasse
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Correspondence to Emilie Cardot Martin.

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Highlights

ID-FUNGI plates improve the identification of fungi recovered from respiratory samples by MALDI Biotyper, simplifying workflow in medical laboratories.

Several inoculation methods can be used with ID-FUNGI plates.

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Cardot Martin, E., Renaux, C., Catherinot, E. et al. Rapid identification of fungi from respiratory samples by Bruker Biotyper matrix–assisted laser desorption/ionisation time-of-flight using ID-FUNGI plates. Eur J Clin Microbiol Infect Dis 40, 391–395 (2021). https://doi.org/10.1007/s10096-020-04007-2

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  • DOI: https://doi.org/10.1007/s10096-020-04007-2

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