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Use of Andromas and Bruker MALDI-TOF MS in the identification of Neisseria

European Journal of Clinical Microbiology & Infectious Diseases volume 37, pages 2273–2277 (2018)Cite this article

Abstract

Through the past decade, MALDI-TOF MS has been recognized as a fast and robust tool for identification of most bacteria in clinical microbiology. However, the accuracy of this method to identify Neisseria species is still debated, and few data are available about commensal Neisseria species identification. In this study, we assessed two MALDI-TOF MS systems (Bruker Biotyper and Andromas) for the identification of 88, 18, and 29 isolates of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species, respectively. All 88 isolates of N. gonorrhoeae were correctly identified using both systems, and most N. meningitidis and commensal Neisseria species were well identified: only 1/18 isolates of N. meningitidis was misidentified using Bruker Biotyper, and 1 isolate of Neisseria polysaccharea was misidentified as N. meningitidis using both systems. These results strengthen the possibility to use MALDI-TOF MS as a single method for Neisseria identification in routine, with excellent performance for N. gonorrhoeae identification. However, results should be interpreted prudently for N. meningitdis and commensal Neisseria species when isolated from genital and oropharyngeal samples where these both species can coexist.

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References

  1. Liu G, Tang CM, Exley RM (2015) Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus. Microbiol Read Engl 161:1297–1312

    CAS  Article  Google Scholar 

  2. Alexander S, Ison C (2005) Evaluation of commercial kits for the identification of Neisseria gonorrhoeae. J Med Microbiol 54:827–831

    Article  Google Scholar 

  3. Dossett JH, Appelbaum PC, Knapp JS, Totten PA (1985) Proctitis associated with Neisseria cinerea misidentified as Neisseria gonorrhoeae in a child. J Clin Microbiol 21:575–577

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Kratz MM, Weiss D, Ridpath A, Zucker JR, Geevarughese A, Rakeman J, Varma JK (2015) Community-based outbreak of Neisseria meningitidis serogroup C infection in men who have sex with men, New York City, New York, USA, 2010-2013. Emerg Infect Dis 21:1379–1386

    CAS  Article  Google Scholar 

  5. Bille E, Dauphin B, Leto J, Bougnoux M-E, Beretti J-L, Lotz A, Suarez S, Meyer J, Join-Lambert O, Descamps P, Grall N, Mory F, Dubreuil L, Berche P, Nassif X, Ferroni A (2012) MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures. Clin Microbiol Infect Dis 18:1117–1125

    CAS  Article  Google Scholar 

  6. Carbonnelle E, Grohs P, Jacquier H, Day N, Tenza S, Dewailly A, Vissouarn O, Rottman M, Herrmann J-L, Podglajen I, Raskine L (2012) Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification. J Microbiol Methods 89:133–136

    CAS  Article  Google Scholar 

  7. Buchanan R, Ball D, Dolphin H, Dave J (2016) Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the identification of Neisseria gonorrhoeae. Clin Microbiol Infect Dis 22:815

    CAS  Article  Google Scholar 

  8. Carannante A, De Carolis E, Vacca P, Vella A, Vocale C, De Francesco MA, Cusini M, Del Re S, Dal Conte I, Cristaudo A, Ober P, Sanguinetti M, Stefanelli P (2015) Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and clustering of Neisseria gonorrhoeae. BMC Microbiol 15:142

    Article  Google Scholar 

  9. Ilina EN, Borovskaya AD, Malakhova MM, Vereshchagin VA, Kubanova AA, Kruglov AN, Svistunova TS, Gazarian AO, Maier T, Kostrzewa M, Govorun VM (2009) Direct bacterial profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria. J Mol Diagn 11:75–86

    CAS  Article  Google Scholar 

  10. Vironneau P, Kania R, Cambau E, Berçot B (2013) Neisseria polysaccharea and Neisseria cinerea identified like Neisseria meningitidis by MALDI-TOF. Germany, Berlin

    Google Scholar 

  11. Schubert S, Weinert K, Wagner C, Gunzl B, Wieser A, Maier T, Kostrzewa M (2011) Novel, improved sample preparation for rapid, direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. J Mol Diagn 13:701–706

    CAS  Article  Google Scholar 

  12. Bennett JS, Jolley KA, Earle SG, Corton C, Bentley SD, Parkhill J, Maiden MCJ (2012) A genomic approach to bacterial taxonomy: an examination and proposed reclassification of species within the genus Neisseria. Microbiol Read Engl 158:1570–1580

    CAS  Article  Google Scholar 

  13. Harrison OB, Schoen C, Retchless AC, Wang X, Jolley KA, Bray JE, Maiden MCJ (2017) Neisseria genomics: current status and future perspectives. Pathog Dis 75

  14. Schweitzer VA, van Dam AP, Hananta IPY, Schuurman R, Kusters JG, Rentenaar RJ (2016) Identification of Neisseria gonorrhoeae by the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system is improved by a database extension. J Clin Microbiol 54:1130–1132

    CAS  Article  Google Scholar 

  15. Golparian D, Dauphin B, Hellmark B, et al P2.046 Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for species identification within the Neisseria genus - effective alternative to conventional techniques? Sex Transm Infect 2013;89:A102

    Google Scholar 

  16. Zhu P, Tsang RSW, Tsai C-M (2003) Nonencapsulated Neisseria meningitidis strain produces amylopectin from sucrose: altering the concept for differentiation between N. meningitidis and N. polysaccharea. J Clin Microbiol 41:273–278

    CAS  Article  Google Scholar 

  17. Boquete MT, Marcos C, Sáez-Nieto JA (1986) Characterization of Neisseria polysacchareae sp. nov. (Riou, 1983) in previously identified noncapsular strains of Neisseria meningitidis. J Clin Microbiol 23:973–975

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Cunningham SA, Mainella JM, Patel R (2014) Misidentification of Neisseria polysaccharea as Neisseria meningitidis with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 52:2270–2271

    CAS  Article  Google Scholar 

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Authors and Affiliations

  1. Département des Agents Infectieux, Hôpitaux Universitaires Saint-Louis, Lariboisière, Fernand-Widal, APHP, Paris, France

    Florence Morel, Hervé Jacquier, Sylvain Kumanski, Emmanuelle Cambau & Béatrice Berçot

  2. Infection, Antimicrobiens, Modélisation, Evolution (IAME), UMR1137, INSERM, Université Paris Diderot, Paris, France

    Florence Morel, Hervé Jacquier, Emmanuelle Cambau & Béatrice Berçot

  3. Unité de Bactériologie-Hygiène, Hôpitaux Universitaires Henri Mondor, APHP, Paris, France

    Marine Desroches, Vincent Fihman & Jean-Winoc Decousser

  4. EA Dynamyc, Université Paris Est Créteil– Ecole vétérinaire de Maison Alfort, F-94000, Créteil, France

    Vincent Fihman

Authors
  1. Florence Morel
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  2. Hervé Jacquier
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  3. Marine Desroches
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  4. Vincent Fihman
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  5. Sylvain Kumanski
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  6. Emmanuelle Cambau
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  7. Jean-Winoc Decousser
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  8. Béatrice Berçot
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Correspondence to Florence Morel.

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Morel, F., Jacquier, H., Desroches, M. et al. Use of Andromas and Bruker MALDI-TOF MS in the identification of Neisseria. Eur J Clin Microbiol Infect Dis 37, 2273–2277 (2018). https://doi.org/10.1007/s10096-018-3368-6

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  • DOI: https://doi.org/10.1007/s10096-018-3368-6

Keywords

  • Bacterial identification
  • Neisseria meningitidis
  • Neisseria gonorrhoeae
  • Commensal Neisseria species
  • Matrix-assisted laser desorption ionization-time of flight mass spectrometry
  • Bruker Biotyper MALDI-TOF
  • Andromas MALDI-TOF