Abstract
Detection of Salmonella Paratyphi A stool carriers by conventional stool culture media is hindered by the absence of hydrogen sulphide production compared to most other Salmonella serovars. This study evaluated the detection of Salmonella Paratyphi A in stool samples using Salmonella chromogenic media compared to a conventional medium. Four chromogenic media, COMPASS Salmonella agar (Biokar Diagnostics, Beauvais, France), BBL™ CHROMagar™ Salmonella (BD Diagnostics, Erembodegem-Aalst, Belgium), Brilliance™ Salmonella agar (Oxoid Ltd., Basingstoke, UK) and Salmonella PLUS CHROMagar™ (CHROMagar, Paris, France), were compared to conventional Salmonella-Shigella agar (Oxoid Ltd.). The colony morphology of 29 freshly grown stock isolates (Salmonella and competing organisms) was assessed. The limit of detection (LOD) was also determined using saline and stool suspensions. Finally, recognizability of Salmonella Paratyphi A isolates was assessed using 20 human stool samples spiked with different concentrations of Salmonella Paratyphi A. All Salmonella Paratyphi A isolates demonstrated detectable growth with typical purple-coloured colonies that could be clearly differentiated from competing organisms on all four chromogenic media. The LOD for Salmonella Paratyphi A was 103 colony-forming units (CFU)/ml for all media, except for Brilliance agar (105 CFU/ml of stool). Salmonella Paratyphi A was easy to differentiate from competing organisms in the spiked stool samples. Colony yields improved when an enrichment step (Selenite-F broth (BD Diagnostics, Erembodegem-Aalst, Belgium)) and prolonged incubation for 48 h were performed. Chromogenic media demonstrated good specificity and a low LOD for the detection of Salmonella Paratyphi A in stool samples.
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References
Baker S, Karkey A, Parry C (2014) Are we adequately prepared for the emergence of Salmonella enterica serovar Paratyphi A? Lancet Glob Health 2(4):e195–e196
Khatri NS, Maskey P, Poudel S, Jaiswal VK, Karkey A, Koirala S et al (2009) Gallbladder carriage of Salmonella Paratyphi A may be an important factor in the increasing incidence of this infection in South Asia. Ann Intern Med 150(8):567–568
van Dijk S, Bruins MJ, Ruijs GJ (2009) Evaluation and implementation of a chromogenic agar medium for Salmonella detection in stool in routine laboratory diagnostics. J Clin Microbiol 47(2):456–458
Becton Dickinson (BD) (2013) Instructions for use—ready-to-use plated media BD: Brilliant Green Agar. PA-212097.07. Rev. Heidelberg, Germany
Cassar R, Cuschieri P (2003) Comparison of Salmonella chromogenic medium with DCLS agar for isolation of Salmonella species from stool specimens. J Clin Microbiol 41(7):3229–3232
Church DL, Emshey D, Lloyd T, Pitout J (2010) Clinical and economic evaluation of BBL CHROMagar Salmonella (CHROMSal) versus subculture after selenite broth enrichment to CHROMSal and Hektoen enteric agars to detect enteric Salmonella in a large regional microbiology laboratory. Diagn Microbiol Infect Dis 68(1):13–19
Perez JM, Cavalli P, Roure C, Renac R, Gille Y, Freydiere AM (2003) Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools. J Clin Microbiol 41(3):1130–1134
Maddocks S, Olma T, Chen S (2002) Comparison of CHROMagar Salmonella medium and xylose-lysine-desoxycholate and Salmonella-Shigella agars for isolation of Salmonella strains from stool samples. J Clin Microbiol 40(8):2999–3003
Gaillot O, di Camillo P, Berche P, Courcol R, Savage C (1999) Comparison of CHROMagar Salmonella medium and hektoen enteric agar for isolation of salmonellae from stool samples. J Clin Microbiol 37(3):762–765
Merselis JG Jr, Kaye D, Connolly CS, Hook EW (1964) Quantitative bacteriology of the typhoid carrier state. Am J Trop Med Hyg 13:425–429
Becton Dickinson (BD) (2013) BD™ Salmonella Shigella Agar. Instructions for use—ready-to-use plated media PA-254047.06. Rev. Heidelberg, Germany
Biokar Diagnostics COMPASS Salmonella agar. Code document: BM066/A/2003–03: 8. Beauvais Cedex. France
Becton Dickinson (BD) (2008) BBL™ CHROMagar™ Salmonella. 8010557. Rev. 01. Heidelberg, Germany
Oxoid Ltd. Prepared media—ready prepared plates. Brilliance Salmonella agar. P05098A. Basingstoke, United Kingdom
CHROMagar. CHROMagar™ Salmonella Plus. Instructions For Use. NT-EXT-024. Version 10. Paris, France
Grimont PA, Weill F-X (2007) Antigenic formulae of the Salmonella serovars, 9th edn. Institut Pasteur, Paris Available from: http://www.scacm.org/free/Antigenic%20Formulae%20of%20the%20Salmonella%20Serovars%202007%209th%20edition.pdf. Last accessed on 05-05-2018
Eigner U, Reissbrodt R, Hammann R, Fahr AM (2001) Evaluation of a new chromogenic medium for the isolation and presumptive identification of Salmonella species from stool specimens. Eur J Clin Microbiol Infect Dis 20(8):558–565
Martiny D, Dediste A, Anglade C, Vlaes L, Moens C, Mohamed S et al (2016) Performance of the chromID Salmonella elite chromogenic agar in comparison with CHROMagar Salmonella, Oxoid brilliance Salmonella and Hektoen agars for the isolation of Salmonella from stool specimens. Diagn Microbiol Infect Dis 86(2):128–130
Lab M (Neogen). Harlequin™ Salmonella ABC medium. Product code: HAL001. Lancashire, United Kingdom
Gunn JS, Marshall JM, Baker S, Dongol S, Charles RC, Ryan ET (2014) Salmonella chronic carriage: epidemiology, diagnosis, and gallbladder persistence. Trends Microbiol 22(11):648–655
Acknowledgements
We would like to thank Marleen Verlinden of the Central Laboratory of Clinical Biology (ITM, Antwerp) for technical support and Kara Osbak and Michael Naan for language editing.
Funding
LMFK was supported by the Flemish Ministry of Sciences (EWI, SOFI project IDIS) (http://www.ewi-vlaanderen.be/en).
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The use of clinical stool samples for the purpose of this study was approved by the Institutional Review Board of the ITM (ref. IRB/AB/ac/019, dated August 1, 2015).
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The authors declare that they have no conflict of interest.
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Informed consent was not necessary from patients as it concerned leftover anonymized samples collected during routine care.
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Kuijpers, L.M.F., Post, A.S. & Jacobs, J. Chromogenic media for the detection of Salmonella enterica serovar Paratyphi A in human stool samples: evaluation in a reference setting. Eur J Clin Microbiol Infect Dis 37, 2181–2190 (2018). https://doi.org/10.1007/s10096-018-3360-1
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DOI: https://doi.org/10.1007/s10096-018-3360-1