Capsular genotype and lipooligosaccharide locus class distribution in Campylobacter jejuni from young children with diarrhea and asymptomatic carriers in Bangladesh

  • Z. Islam
  • S. K. Sarker
  • I. Jahan
  • K. S. Farzana
  • D. Ahmed
  • A. S. G. Faruque
  • P. Guerry
  • F. Poly
  • A. P. Heikema
  • H. P. Endtz
Original Article

Abstract

Campylobacter jejuni-related diarrheal diseases is one of the major health issues among young children (0–59 months old) in low-income countries. Monitoring of the capsular (capsule polysaccharide, CPS) types of virulent C. jejuni strains in regions where the disease is endemic is of great importance for the development of a customized capsule-based multivalent vaccine. Therefore, we aimed to determine the prevalence of CPS genotypes among C. jejuni strains isolated from young children with enteritis (n = 152) and asymptomatic carriers matched by age, sex, and residence defined as the control group (n = 215) in Bangladesh. CPS genotyping was performed using a newly established multiplex polymerase chain reaction (PCR) method and lipooligosaccharide (LOS) locus classes (A–E) were characterized using PCR as well. We identified 24 different CPS genotypes among the 367 isolates. Four prevalent capsular types, HS5/31 complex (n = 27, 18%), HS3 (n = 26, 17%), HS4A (n = 10, 7%), and HS8/17 (n = 10, 7%) covered almost 50% of the strains from enteritis patients and 43% of the isolates from controls. In combination, the CPS genotype and LOS class was not discriminative between cases and controls. Dominant capsular types previously identified in C. jejuni strains isolated from patients with Guillain–Barré syndrome in Bangladesh were rarely detected in strains isolated from the young children. A similar distribution was evident among enteritis- and control-related strains when comparison was done between CPS types and LOS classes. This is the first systematic study presenting the distribution of CPS genotypes of C. jejuni strains isolated in Bangladesh from children with diarrhea and controls, with capsular genotypes HS5/31 complex, HS3, HS4A, and HS8/17 being prevalent in both. In conclusion, systematic studies are required to develop a multivalent capsule-based vaccine for children in low-income countries.

Notes

Acknowledgements

This research study was funded by icddr,b's core donors through mentoring program. icddr,b also gratefully acknowledges the following donors who provide unrestricted support: the Government of the People's Republic of Bangladesh, Global Affairs Canada (GAC), Swedish International Development Cooperation Agency (Sida), and the Department for International Development (UK Aid).

Compliance with Ethical Standards

Conflict of Interest

The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Z. Islam
    • 1
  • S. K. Sarker
    • 1
  • I. Jahan
    • 1
  • K. S. Farzana
    • 1
  • D. Ahmed
    • 1
  • A. S. G. Faruque
    • 2
  • P. Guerry
    • 3
  • F. Poly
    • 3
  • A. P. Heikema
    • 4
  • H. P. Endtz
    • 1
    • 4
    • 5
  1. 1.Laboratory Sciences and Services DivisionInternational Centre for Diarrhoeal Disease Research (icddr,b)DhakaBangladesh
  2. 2.Nutrition and Clinical Services DivisionInternational Centre for Diarrhoeal Disease Research, (icddr,b)DhakaBangladesh
  3. 3.Naval Medical Research CenterSilver SpringUSA
  4. 4.Department of Medical Microbiology and Infectious DiseasesErasmus MC, University Medical Centre RotterdamRotterdamThe Netherlands
  5. 5.Fondation MérieuxLyonFrance

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