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Bartonella henselae is usually not viable in lymph nodes of patients with cat scratch disease

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Abstract

Bartonella henselae, the agent of cat scratch disease (CSD), appears to be a common organism responsible for lymphadenitis in both adults and children. There is a very low isolation rate for B. henselae from lymph nodes of patients with CSD. Our objective was to evaluate B. henselae viability in a large series of lymph nodes from patients with CSD. From January to November 2016, we analyzed lymph node biopsy samples from patients diagnosed with CSD. We used reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to detect B. henselae RNA, as well as cultures, histological analyses, and fluorescence in situ hybridization (FISH). We tested 87 lymph nodes positive for B. henselae DNA but only 8 (9%) presented with B. henselae RNA. We did not find a significant difference for the pap threshold cycle (CT) values between RNA-positive and RNA-negative lymph nodes (p = 0.5). Cultures, histological analyses, and FISH were negative for all the tested samples. We provide evidence that B. henselae are not or are rarely viable in most cases in the lymph nodes of patients with CSD.

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References

  1. Amann RI, Krumholz L, Stahl DA (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol 172:762–770

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Angelakis E, Edouard S, La Scola B, Raoult D (2010) Bartonella henselae in skin biopsy specimens of patients with cat-scratch disease. Emerg Infect Dis 16:1963–1965

    Article  PubMed  PubMed Central  Google Scholar 

  3. Angelakis E, Raoult D (2014) Pathogenicity and treatment of Bartonella infections. Int J Antimicrob Agents 44:16–25

    Article  CAS  PubMed  Google Scholar 

  4. Angelakis E, Roux V, Raoult D, Rolain J-M (2009) Real-time PCR strategy and detection of bacterial agents of lymphadenitis. Eur J Clin Microbiol Infect Dis 28:1363–1368

    Article  CAS  PubMed  Google Scholar 

  5. Audoly G, Vincentelli R, Edouard S et al (2011) Effect of rickettsial toxin VapC on its eukaryotic host. PLoS One 6:e26528

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Bass JW, Freitas BC, Freitas AD et al (1998) Prospective randomized double blind placebo-controlled evaluation of azithromycin for treatment of cat-scratch disease. Pediatr Infect Dis J 17:447–452

    Article  CAS  PubMed  Google Scholar 

  7. Boulouis H-J, Haddad N, Vayssier-Taussat M, Maillard R, Chomel B (2007) Persistent Bartonella infection: epidemiological and clinical implications. Bull Acad Natl Med 191:1037–1044; discussion 1047–1049

    PubMed  Google Scholar 

  8. Breitschwerdt EB, Kordick DL (2000) Bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection. Clin Microbiol Rev 13:428–438

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Brenner DJ, Hollis DG, Moss CW et al (1991) Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies. J Clin Microbiol 29:2450–2460

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Brenner DJ, O’Connor SP, Winkler HH, Steigerwalt AG (1993) Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int J Syst Bacteriol 43:777–786

    Article  CAS  PubMed  Google Scholar 

  11. Brock TD (1999) Robert Koch: a life in medicine and bacteriology. ASM Press, Washington, DC

    Book  Google Scholar 

  12. Cenciarini C, Courtois S, Raoult D, La Scola B (2008) Influence of long time storage in mineral water on RNA stability of Pseudomonas aeruginosa and Escherichia coli after heat inactivation. PLoS One 3:e3443

    Article  PubMed  PubMed Central  Google Scholar 

  13. Debré R, Job J-C (1954) Cat scratch fever. Acta Paediatr 43:386–389

    Article  Google Scholar 

  14. Edouard S, Million M, Lepidi H et al (2013) Persistence of DNA in a cured patient and positive culture in cases with low antibody levels bring into question diagnosis of Q fever endocarditis. J Clin Microbiol 51:3012–3017

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Edouard S, Raoult D (2010) Bartonella henselae, un agent d’infections ubiquitaires. Med Mal Infect 40:319–330

    Article  CAS  PubMed  Google Scholar 

  16. Eldin C, Angelakis E, Renvoisé A, Raoult D (2013) Coxiella burnetii DNA, but not viable bacteria, in dairy products in France. Am J Trop Med Hyg 88:765–769

    Article  PubMed  PubMed Central  Google Scholar 

  17. English CK, Wear DJ, Margileth AM, Lissner CR, Walsh GP (1988) Cat-scratch disease. Isolation and culture of the bacterial agent. JAMA 259:1347–1352

    Article  CAS  PubMed  Google Scholar 

  18. Gouriet F, Fenollar F, Patrice J-Y, Drancourt M, Raoult D (2005) Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience. J Clin Microbiol 43:4993–5002

    Article  PubMed  PubMed Central  Google Scholar 

  19. Gouriet F, Lepidi H, Habib G, Collart F, Raoult D (2007) From cat scratch disease to endocarditis, the possible natural history of Bartonella henselae infection. BMC Infect Dis 7:30

    Article  PubMed  PubMed Central  Google Scholar 

  20. Gradidge E, Chauhan A, Steele RW, Warrier R (2013) Fever, lymphadenopathy, and splenomegaly: did the cat do it? Clin Pediatr (Phila) 52:1072–1074

    Article  Google Scholar 

  21. Jabcuga CE, Jin L, Macon WR, Howard MT, Oliveira AM, King RL (2016) Broadening the morphologic spectrum of Bartonella henselae lymphadenitis: analysis of 100 molecularly characterized cases. Am J Surg Pathol 40:342–347

    Article  PubMed  Google Scholar 

  22. Kempf VAJ, Schaller M, Behrendt S et al (2000) Interaction of Bartonella henselae with endothelial cells results in rapid bacterial rRNA synthesis and replication. Cell Microbiol 2:431–441

    Article  CAS  PubMed  Google Scholar 

  23. Kunz S, Oberle K, Sander A, Bogdan C, Schleicher U (2008) Lymphadenopathy in a novel mouse model of Bartonella-induced cat scratch disease results from lymphocyte immigration and proliferation and is regulated by interferon-α/β. Am J Pathol 172:1005–1018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. La Scola B, Raoult D (1999) Afipia felis in hospital water supply in association with free-living amoebae. Lancet 353:1330

    Article  PubMed  Google Scholar 

  25. La Scola B, Raoult D (1999) Culture of Bartonella quintana and Bartonella henselae from human samples: a 5-year experience (1993 to 1998). J Clin Microbiol 37:1899–1905

    PubMed  PubMed Central  Google Scholar 

  26. Leone M, Honstettre A, Lepidi H et al (2004) Effect of sex on Coxiella burnetii infection: protective role of 17β-estradiol. J Infect Dis 189:339–345

    Article  CAS  PubMed  Google Scholar 

  27. Lepidi H, Fournier P-E, Raoult D (2000) Quantitative analysis of Valvular lesions during Bartonella endocarditis. Am J Clin Pathol 114:880–889

    Article  CAS  PubMed  Google Scholar 

  28. Mallmann C, Siemoneit S, Schmiedel D et al (2010) Fluorescence in situ hybridization to improve the diagnosis of endocarditis: a pilot study. Clin Microbiol Infect 16:767–773

    Article  CAS  PubMed  Google Scholar 

  29. Margileth AM (1992) Antibiotic therapy for cat-scratch disease: clinical study of therapeutic outcome in 268 patients and a review of the literature. Pediatr Infect Dis J 11:474–478

    Article  CAS  PubMed  Google Scholar 

  30. Margileth AW, Wear DJ, Hadfield TL, Schlagel CJ, Spigel GT, Muhlbauer JE (1984) Cat-scratch disease. Bacteria in skin at the primary inoculation site. JAMA 252:928–931

    Article  CAS  PubMed  Google Scholar 

  31. Morel A-S, Dubourg G, Prudent E et al (2015) Complementarity between targeted real-time specific PCR and conventional broad-range 16S rDNA PCR in the syndrome-driven diagnosis of infectious diseases. Eur J Clin Microbiol Infect Dis 34:561–570

    Article  CAS  PubMed  Google Scholar 

  32. Prutsky G, Domecq JP, Mori L et al (2013) Treatment outcomes of human bartonellosis: a systematic review and meta-analysis. Int J Infect Dis 17:e811–e819

    Article  PubMed  Google Scholar 

  33. Regnery R, Martin M, Olson J (1992) Naturally occurring “Rochalimaea henselae” infection in domestic cat. Lancet 340:557–558

    Article  CAS  PubMed  Google Scholar 

  34. Regnery RL, Olson JG, Perkins BA, Bibb W (1992) Serological response to “Rochalimaea henselae” antigen in suspected cat-scratch disease. Lancet 339:1443–1445

    Article  CAS  PubMed  Google Scholar 

  35. Resto-Ruiz S, Burgess A, Anderson BE (2003) The role of the host immune response in pathogenesis of Bartonella henselae. DNA Cell Biol 22:431–440

    Article  CAS  PubMed  Google Scholar 

  36. Ridder GJ, Boedeker CC, Technau-Ihling K, Grunow R, Sander A (2002) Role of cat-scratch disease in lymphadenopathy in the head and neck. Clin Infect Dis 35:643–649

    Article  PubMed  Google Scholar 

  37. Rolain JM, Brouqui P, Koehler JE, Maguina C, Dolan MJ, Raoult D (2004) Recommendations for treatment of human infections caused by Bartonella species. Antimicrob Agents Chemother 48:1921–1933

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Rolain JM, Gouriet F, Enea M, Aboud M, Raoult D (2003) Detection by immunofluorescence assay of Bartonella henselae in lymph nodes from patients with cat scratch disease. Clin Diagn Lab Immunol 10:686–691

    CAS  PubMed  PubMed Central  Google Scholar 

  39. Rolain J-M, Lepidi H, Zanaret M et al (2006) Lymph node biopsy specimens and diagnosis of cat-scratch disease. Emerg Infect Dis 12:1338–1344

    Article  PubMed  PubMed Central  Google Scholar 

  40. Rovery C, Greub G, Lepidi H et al (2005) PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. J Clin Microbiol 43:163–167

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Safont M, Angelakis E, Richet H et al (2014) Bacterial lymphadenitis at a major referral hospital in France from 2008 to 2012. J Clin Microbiol 52:1161–1167

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Shinall EA (1990) Cat-scratch disease: a review of the literature. Pediatr Dermatol 7:11–18

    Article  CAS  PubMed  Google Scholar 

  43. Sung K, Hiett KL, Stern NJ (2005) Heat-treated Campylobacter spp. and mRNA stability as determined by reverse transcriptase-polymerase chain reaction. Foodborne Pathog Dis 2:130–137

    Article  CAS  PubMed  Google Scholar 

  44. Vayssier-Taussat M, Moutailler S, Féménia F et al (2016) Identification of novel zoonotic activity of Bartonella spp., France. Emerg Infect Dis 22:457–462

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Vermi W, Facchetti F, Riboldi E et al (2006) Role of dendritic cell-derived CXCL13 in the pathogenesis of Bartonella henselae B-rich granuloma. Blood 107:454–462

    Article  CAS  PubMed  Google Scholar 

  46. Wallner G, Amann R, Beisker W (1993) Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136–143

    Article  CAS  PubMed  Google Scholar 

  47. Wear DJ, Margileth AM, Hadfield TL, Fischer GW, Schlagel CJ, King FM (1983) Cat scratch disease: a bacterial infection. Science 221:1403–1405

    Article  CAS  PubMed  Google Scholar 

  48. Yaron S, Matthews KR (2002) A reverse transcriptase-polymerase chain reaction assay for detection of viable Escherichia coli O157:H7: investigation of specific target genes. J Appl Microbiol 92:633–640

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. URMITE, Aix Marseille Université, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - Méditerranée Infection, 19–21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France

    E. Prudent, H. Lepidi, G. Audoly, B. La Scola, P.-E. Fournier, S. Edouard, E. Angelakis & D. Raoult

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  1. E. Prudent
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  2. H. Lepidi
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  3. G. Audoly
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  6. S. Edouard
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  7. E. Angelakis
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  8. D. Raoult
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Correspondence to D. Raoult.

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Elsa Prudent is a researcher at URMITE UMR 6236, CNRS-IRD in Marseille. Her research interests are zoonotic pathogens.

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Prudent, E., Lepidi, H., Audoly, G. et al. Bartonella henselae is usually not viable in lymph nodes of patients with cat scratch disease. Eur J Clin Microbiol Infect Dis 36, 2207–2213 (2017). https://doi.org/10.1007/s10096-017-3047-z

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  • DOI: https://doi.org/10.1007/s10096-017-3047-z

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