Molecular xenomonitoring (MX) and transmission assessment survey (TAS) of lymphatic filariasis elimination in two villages, Menoufyia Governorate, Egypt
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Section (1): a school-based survey where all the primary school entrants (6–7) years of age were tested by ICT.
Section (2): a mosquito-based survey where a total of 152 mosquito pools collected from Samalay and 167 from Kafr El-Tarainah were tested for the presence of the gDNA of Wuchereria bancrofti microfilaria by real-time PCR assays.
KeywordsLymphatic Filariasis Mass Drug Administration Primary School Child Culex Pipiens Filarial Antigen
Precious assistance was provided by the Egyptian Ministry of Health and Population. The W. bancrofti amplified DNA whole genome was obtained through the Filariasis Research Reagent Resource Center (FR3), Division of Microbiology and infectious Diseases, NIAID, NIH. Also we would like to thank Ahmed Mohamed Shams El-deen for his participation in the field work of this study.
Compliance with ethical standards
This work was sponsored by the WHO Regional Office for the Eastern Mediterranean, in collaboration with the Special Program for Research and Training in Tropical Diseases (TDR), Joint EMRO/TDR Small Grants Scheme for implementation research in communicable diseases (grant no. ID SGS 13/58). The sponsor of the study reviewed the study protocol to ensure compliance with good clinical practice standards. Otherwise, the sponsor had no role in the study design, data collection, data analysis, and data interpretation.
Conflict of interest
The authors declare that they have no conflict of interest.
The study design was reviewed and approved by the Research Ethical Committee (REC), Faculty of Medicine Ain Shams University.
Informed consent was obtained from all individual participants included in the study.
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