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In vitro blood culture bottle inoculation of whole blood with clinically relevant antibiotic concentrations: a word of caution

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References

  1. Lovern D, Katzin B, Johnson K, Broadwell D, Miller E, Gates A, Deol P, Doing K, van Belkum A, Marshall C, Mathias E, Dunne WM Jr (2016) Antimicrobial binding and growth kinetics in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture media. Eur J Clin Microbiol Infect Dis 35:2033–2036. doi:10.1007/s10096-016-2759-9

    Article  CAS  PubMed  Google Scholar 

  2. Goto M, Al-Hasan MN (2013) Overall burden of bloodstream infection and nosocomial bloodstream infection in North America and Europe. Clin Microbiol Infect 19:501–509

    Article  CAS  PubMed  Google Scholar 

  3. Laupland KB (2013) Incidence of bloodstream infection: a review of population-based studies. Clin Microbiol Infect 19:492–500

    Article  CAS  PubMed  Google Scholar 

  4. Laupland KB, Church DL (2014) Population-based epidemiology and microbiology of community-onset bloodstream infections. Clin Microbiol Rev 27:647–664

    Article  PubMed  PubMed Central  Google Scholar 

  5. Søgaard M, Lyytikäinen O, Laupland KB, Schønheyder HC (2013) Monitoring the epidemiology of bloodstream infections: aims, methods and importance. Expert Rev Anti Infect Ther 11:1281–1290

    Article  PubMed  Google Scholar 

  6. Tumbarello M, Sanguinetti M, Montuori E, Trecarichi EM, Posteraro B, Fiori B, Citton R, D’Inzeo T, Fadda G, Cauda R, Spanu T (2007) Predictors of mortality in patients with bloodstream infections caused by extended-spectrum-beta-lactamase-producing Enterobacteriaceae: importance of inadequate initial antimicrobial treatment. Antimicrob Agents Chemother 51:1987–1994

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Barenfanger J, Graham DR, Kolluri L, Sangwan G, Lawhorn J, Drake CA, Verhulst SJ, Peterson R, Moja LB, Ertmoed MM, Moja AB, Shevlin DW, Vautrain R, Callahan CD (2008) Decreased mortality associated with prompt gram staining of blood cultures. Am J Clin Pathol 130:870–876

    Article  PubMed  Google Scholar 

  8. Funk DJ, Kumar A (2011) Antimicrobial therapy for life-threatening infections: speed is life. Crit Care Clin 27:53–76

    Article  PubMed  Google Scholar 

  9. Dubourg G, Raoult D (2016) Emerging methodologies for pathogen identification in positive blood culture testing. Expert Rev Mol Diagn 16:97–111

    Article  CAS  PubMed  Google Scholar 

  10. Spaargaren J, van Boven CP, Voorn GP (1998) Effectiveness of resins in neutralizing antibiotic activities in bactec plus Aerobic/F culture medium. J Clin Microbiol 36:3731–3733

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Chern IF, Steer JA, Wilson AP (1999) Effect of teicoplanin on isolation of Staphylococcus aureus from blood culture media. J Antimicrob Chemother 43:589–591

    Article  CAS  PubMed  Google Scholar 

  12. Viganò EF, Vasconi E, Agrappi C, Clerici P, Melloni P (2004) Use of simulated blood cultures for antibiotic effect on time to detection of the two blood culture systems BacT/ALERT and BACTEC 9240. New Microbiol 27:235–248

    PubMed  Google Scholar 

  13. Flayhart D, Borek AP, Wakefield T, Dick J, Carroll KC (2007) Comparison of BACTEC PLUS blood culture media to BacT/Alert FA blood culture media for detection of bacterial pathogens in samples containing therapeutic levels of antibiotics. J Clin Microbiol 45:816–821

    Article  CAS  PubMed  Google Scholar 

  14. Miller NS, Rogan D, Orr BL, Whitney D (2011) Comparison of BD Bactec Plus blood culture media to VersaTREK Redox blood culture media for detection of bacterial pathogens in simulated adult blood cultures containing therapeutic concentrations of antibiotics. J Clin Microbiol 49:1624–1627. doi:10.1128/JCM.01958-10

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Mitteregger D, Barousch W, Nehr M, Kundi M, Zeitlinger M, Makristathis A, Hirschl AM (2013) Neutralization of antimicrobial substances in new BacT/Alert FA and FN Plus blood culture bottles. J Clin Microbiol 51:1534–1540. doi:10.1128/JCM.00103-13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Sutherland CA, Nicolau DP (2016) Development of an HPLC method for the determination of ceftolozane/tazobactam in biological and aqueous matrixes. J Chromatogr Sci 54:1037–1040. doi:10.1093/chromsci/bmw047

    Article  CAS  PubMed  Google Scholar 

  17. Merck & Co. (2015) Zerbaxa®. https://www.merck.com/product/usa/pi_circulars/z/zerbaxa/zerbaxa_pi.pdf. Accessed 19 Jul 2016

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Acknowledgments

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Center for Anti-Infective Research and Development, Hartford Hospital, 80 Seymour Street, Hartford, CT, 06102, USA

    M. Grupper, J. L. Kuti & D. P. Nicolau

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  1. M. Grupper
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  2. J. L. Kuti
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  3. D. P. Nicolau
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Correspondence to D. P. Nicolau.

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Grupper, M., Kuti, J.L. & Nicolau, D.P. In vitro blood culture bottle inoculation of whole blood with clinically relevant antibiotic concentrations: a word of caution. Eur J Clin Microbiol Infect Dis 36, 917–919 (2017). https://doi.org/10.1007/s10096-016-2874-7

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  • DOI: https://doi.org/10.1007/s10096-016-2874-7

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