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Prothionamide susceptibility testing of Mycobacterium tuberculosis using the resazurin microtitre assay and the BACTECMGIT 960 system

Abstract

Resazurin microtitre assay (RMA) has been successfully used to detect minimal inhibitory concentrations (MICs) of both first-line and several second-line drugs in drug susceptibility testing (DST) of Mycobacterium tuberculosis (MTB). In this study, we firstly compared prothionamide (PTH) susceptibility testing of Mycobacterium tuberculosis (MTB) using resazurin microtitre assay (RMA) and MGIT. Overall, the sensitivity and specificity of RMA for detecting PTH susceptibility was 96.5% [95% confidence interval (CI): 91.7–100.0] and 93.2% (95% CI: 89.6–96.8) respectively. In addition, the median time to positivity was significantly shorter for RMA than for the automated MGIT 960 (RMA, 8 days [range: 8–8 days] vs MGIT, 10.1 days, [range: 5.0–13.0]; P < 0.01). Concordance rate for MICs between RMA and MGIT for PTH-resistant group was 64.3% (95% CI: 46.5–82.0), which was significantly lower than that of PTH-susceptible group (85.9%, 95% CI: 78.8–93.0; P= 0.01). In conclusion, our data demonstrated that RMA can be used as an acceptable alternative for determination of PTH susceptibility with shorter turn-around time. When compared with MGIT 960, RMA method was prone to produce higher MICs for PTH-resistant MTB strains.

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Acknowledgements

We would like to thank members of the National Tuberculosis Reference Laboratory at the Chinese Center for Disease Control and Prevention for their technical assistance.

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Correspondence to Y. Pang.

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Funding

This work was supported by the National Key Research Program of China (2014ZX10003002).

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study was approved by the Ethics Committee of Beijing Chest Hospital affiliated to Capital Medical University.

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Tan, Y., Su, B., Zheng, H. et al. Prothionamide susceptibility testing of Mycobacterium tuberculosis using the resazurin microtitre assay and the BACTECMGIT 960 system. Eur J Clin Microbiol Infect Dis 36, 779–782 (2017). https://doi.org/10.1007/s10096-016-2859-6

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  • DOI: https://doi.org/10.1007/s10096-016-2859-6

Keywords

  • Concordance Rate
  • Drug Susceptibility Testing
  • High MICs
  • Capreomycin
  • Drug Susceptibility Testing Result