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Spread of carbapenem-resistant international clones of Acinetobacter baumannii in Turkey and Azerbaijan: a collaborative study

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Abstract

Epidemic clones of Acinetobacter baumannii, described as European clones I, II, and III, are associated with hospital epidemics throughout the world. We aimed to determine the molecular characteristics and genetic diversity between European clones I, II, and III from Turkey and Azerbaijan. In this study, a total of 112 bloodstream isolates of carbapenem-resistant Acinetobacter spp. were collected from 11 hospitals across Turkey and Azerbaijan. The identification of Acinetobacter spp. using conventional and sensitivity tests was performed by standard criteria. Multiplex polymerase chain reaction (PCR) was used to detect OXA carbapenemase-encoding genes (bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, and bla OXA-58-like). Pulsed-field gel electrophoresis (PFGE) typing was used to investigate genetic diversity. The bla OXA-51-like gene was present in all 112 isolates, 75 (67 %) carried bla OXA-23-like, 7 (6.2 %) carried bla OXA-58-like genes, and 5 (4.5 %) carried bla OXA-24-like genes. With a 90 % similarity cut-off value, 15 clones and eight unique isolates were identified. The largest clone was cluster D, with six subtypes. Isolates from clusters D and I were widely spread in seven different geographical regions throughout Turkey. However, F cluster was found in the northern and eastern regions of Turkey. EU clone I was grouped within J cluster with three isolates found in Antalya, Istanbul, and Erzurum. EU clone II was grouped in the U cluster with 15 isolates and found in Kayseri and Diyarbakır. The bla OXA-24-like gene in carbapenemases was identified rarely in Turkey and has been reported for the first time from Azerbaijan. Furthermore, this is the first multicenter study in Turkey and Azerbaijan to identify several major clusters belonging to European clones I and II of A. baumannii.

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Acknowledgments

This study has been presented as an e-poster (no. 0890) in the 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Amsterdam, the Netherlands April 2016.

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Authors and Affiliations

  1. Faculty of Infectious Diseases, Erciyes University, Kayseri, Turkey

    S. S. Ahmed, E. Alp, A. Ulu-Kilic & G. Dinc

  2. Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey

    S. S. Ahmed

  3. Dep. of Medical Microbiology, Erciyes University, Kayseri, Turkey

    G. Dinc

  4. Dep. of Infectious Diseases, Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Z. Aktas

  5. Dep. Infectious Diseases, Faculty of Medicine, Ege Univerisity, Izmir, Turkey

    B. Ada

  6. Dep. of Infectious Diseases, Faculty of Medicine, Azerbaijan Medical University, Baku, Azerbaijan

    F. Bagirova

  7. Dep. of Infectious Diseases, Faculty of Medicine, Ankara teaching hospital, Ankara, Turkey

    I. Baran

  8. Dep. of Infectious Diseases, Faculty of Medicine, Inonu University, Malatya, Turkey

    Y. Ersoy

  9. Dep. of Infectious Diseases, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey

    S. Esen

  10. Dep. of Infectious Diseases, Faculty of Medicine, Cokurva University, Adana, Turkey

    T. G. Guven & F. Koksal

  11. Dep. of Medical Microbiology, Radboud UMC, Nijmegen, Netherlands

    J. Hopman, A. Voss & W. Melchers

  12. Dep. of Infectious Diseases, Faculty of Medicine, Trabzon Teaching hospital, Trabzon, Turkey

    S. Hosoglu

  13. Dep. of Infectious Diseases, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    E. Parlak

  14. Dep. of Infectious Diseases, Faculty of Medicine, Akdeniz University, Antalya, Turkey

    A. N. Yalcin

  15. Dep. of Infectious Diseases, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

    G. Yilmaz

Authors
  1. S. S. Ahmed
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  2. E. Alp
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  3. A. Ulu-Kilic
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  4. G. Dinc
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  5. Z. Aktas
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  6. B. Ada
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  7. F. Bagirova
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  8. I. Baran
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  9. Y. Ersoy
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  10. S. Esen
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  11. T. G. Guven
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  12. J. Hopman
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  13. S. Hosoglu
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  14. F. Koksal
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  15. E. Parlak
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  16. A. N. Yalcin
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  17. G. Yilmaz
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  18. A. Voss
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  19. W. Melchers
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Corresponding author

Correspondence to S. S. Ahmed.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was supported by Erciyes University Scientific Research Projects (BAP kodu 4059).

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Approved by the Local Ethics Committee.

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Ahmed, S.S., Alp, E., Ulu-Kilic, A. et al. Spread of carbapenem-resistant international clones of Acinetobacter baumannii in Turkey and Azerbaijan: a collaborative study. Eur J Clin Microbiol Infect Dis 35, 1463–1468 (2016). https://doi.org/10.1007/s10096-016-2685-x

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  • DOI: https://doi.org/10.1007/s10096-016-2685-x

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