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Rapid susceptibility testing for slowly growing nontuberculous mycobacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium WST-1

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Abstract

Rapid susceptibility testing for slowly growing nontuberculous mycobacteria (NTM) using a colorimetric microbial viability assay based on the reduction of the water-soluble tetrazolium salt {2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-1)} using 2,3,5,6-tetramethyl-1,4-benzoquinone as an electron mediator was developed. Using the Clinical and Laboratory Standards Institute (CLSI) method, a long-term incubation time (7–14 days) was required to determine the minimum inhibitory concentrations (MICs) of the slowly growing NTM. The MICs for a variety of different antibiotics against the slowly growing NTM were determined by the WST-1 colorimetric method and compared with those obtained using the broth microdilution methods approved by the CLSI. Good agreement was found between the MICs determined after 3–4 days using the WST-1 colorimetric method and those obtained after 10–14 days using the broth microdilution method. The results suggest that the WST-1 colorimetric assay is a useful method for the rapid determination of the MICs for the slowly growing NTM.

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Acknowledgments

We would like to thank Dr. Satoshi Mitarai (Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association) for several valuable comments and suggestions. This research was supported by the Adaptable and Seamless Technology Transfer Program through Target-Driven R&D (A-STEP) Exploratory Research.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, Kurume, 839-0861, Japan

    T. Tsukatani & H. Suenaga

  2. Dojindo Laboratories, Kumamoto, 861-2202, Japan

    M. Shiga, T. Ikegami, M. Ishiyama & T. Ezoe

  3. Laboratory of Food Bioscience, Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, 860-0082, Japan

    K. Matsumoto

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  1. T. Tsukatani
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  2. H. Suenaga
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Correspondence to T. Tsukatani.

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Tsukatani, T., Suenaga, H., Shiga, M. et al. Rapid susceptibility testing for slowly growing nontuberculous mycobacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium WST-1. Eur J Clin Microbiol Infect Dis 34, 1965–1973 (2015). https://doi.org/10.1007/s10096-015-2438-2

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  • DOI: https://doi.org/10.1007/s10096-015-2438-2

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