Abstract
The diagnosis of Lyme disease is very complicated and a single diagnostic method cannot exclude infection. We assessed the performance of two commercially available Borrelia burgdorferi rapid diagnostic tests (RDT) in comparison to multiple laboratory-based diagnostic assays using specimens with a gradually increasing probability of Borrelia infection. Based on 200 specimens, the analytical sensitivities for IgG and IgM were 18 and 23 % for the Lyme RDT and 24 and 32 % for the Borreliose Complete RDT. The sensitivity for detecting diagnosed Lyme borreliosis cases was low (26 % Lyme RDT and 32 % with the Borreliose Complete RDT respectively), whereas the specificity was good (85 % Lyme RDT and 88 % Borreliose Complete). Based on this evaluation, the performance of RDTs in detecting Lyme borreliosis appeared to be below that of laboratory-based diagnostics.
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We would like to thank Aftab Jasir, Androulla Efstratiou, Triin Pärn and Jaana Vuopio for critically proofreading the manuscript.
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The authors declare that they have no conflict of interest.
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Smit, P.W., Kurkela, S., Kuusi, M. et al. Evaluation of two commercially available rapid diagnostic tests for Lyme borreliosis. Eur J Clin Microbiol Infect Dis 34, 109–113 (2015). https://doi.org/10.1007/s10096-014-2217-5
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DOI: https://doi.org/10.1007/s10096-014-2217-5