Abstract
Generally accepted laboratory methods that have been used for decades do not reliably distinguish between H. influenzae and H. haemolyticus isolates. H. haemolyticus strains are often incorrectly identified as nontypeable Haemophilus influenzae (NTHi). To distinguish H. influenzae from H. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) bio-typer 2 and compared the results with routine determination of Haemophilus (growth requirement for X and V factor), and multilocus sequence typing (MLST). In total we have tested 277 isolates, 244 H. influenzae and 33 H. haemolyticus. Using MLST as the gold standard, the agreement of MALDI-TOF MS was 99.6 %. MALDI-TOF MS allows reliable and rapid discrimination between H. influenzae and H. haemolyticus.
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Funding
This study was funded by The Regional Laboratory of public Health, Haarlem.
Conflict of interest
M. Kostrzewa are employed at the mass spectrometry company Bruker Daltonics GmbH, Bremen, Germany and therefore has potential conflicts of interest. All other authors have declared that no competing interests exist.
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Bruin, J.P., Kostrzewa, M., van der Ende, A. et al. Identification of Haemophilus influenzae and Haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Eur J Clin Microbiol Infect Dis 33, 279–284 (2014). https://doi.org/10.1007/s10096-013-1958-x
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DOI: https://doi.org/10.1007/s10096-013-1958-x