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Evaluation of different chromogenic media for the detection of methicillin-resistant Staphylococcus aureus CC398 in broilers

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Abstract

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has emerged in a wide variety of animal species, including poultry. The objective of this study was to evaluate three different chromogenic media for MRSA clonal complex (CC) 398 detection in broilers. On three Belgian poultry farms, 50 broiler chickens were sampled per farm from both nose shell and cloaca. All swab specimens were enriched and inoculated the following day on three chromogenic media: chromID MRSA (bioMérieux), Brilliance MRSA 2 Agar (Oxoid) and MRSASelect (Bio-Rad). ChromID had the highest isolation rates, yet, Brilliance MRSA 2 Agar demonstrated the highest relative sensitivity, while MRSASelect and Brilliance MRSA 2 Agar showed the highest relative specificity. A subset of MRSA isolates was confirmed to be CC398 by the polymerase chain reaction (PCR) targeting sau1-hsdS1. In conclusion, Brilliance MRSA 2 Agar outperformed MRSASelect and chromID MRSA for the detection of MRSA in broilers.

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Acknowledgements

This research was funded by the Institute for the Promotion of Innovation by Science and Technology (Agentschap voor Innovatie door Wetenschap en Technologie, IWT) in Flanders (Belgium), project 070596. The authors wish to acknowledge Ine Vanherpe, Wim Vanhove and Laurent Lanckman for their help in the collection of the animal samples and technical assistance. Furthermore, we would also like to thank the farmers for their collaboration in this study.

Conflict of interest

The authors declare that they have no conflict of interest and no financial or personal relations with people or organisations that could have inappropriately influenced this work.

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Authors and Affiliations

  1. Department HIVB, Catholic University College South-West-Flanders (KATHO), Wilgenstraat 32, 8800, Roeselare, Belgium

    L. J. Pletinckx, Y. De Bleecker & I. De Man

  2. Department Biosystems, Division Gene Technology, KU Leuven University, Kasteelpark Arenberg 30 - bus 2456, 3001, Heverlee, Belgium

    L. J. Pletinckx & B. M. Goddeeris

  3. Faculty of Veterinary Medicine, Veterinary Epidemiology Unit, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium

    J. Dewulf

  4. Technology and Food Science Unit, Food Safety, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, 9090, Melle, Belgium

    G. Rasschaert

Authors
  1. L. J. Pletinckx
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  2. J. Dewulf
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  3. Y. De Bleecker
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  4. G. Rasschaert
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  5. B. M. Goddeeris
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  6. I. De Man
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Correspondence to L. J. Pletinckx.

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Pletinckx, L.J., Dewulf, J., De Bleecker, Y. et al. Evaluation of different chromogenic media for the detection of methicillin-resistant Staphylococcus aureus CC398 in broilers. Eur J Clin Microbiol Infect Dis 32, 1023–1026 (2013). https://doi.org/10.1007/s10096-013-1844-6

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  • DOI: https://doi.org/10.1007/s10096-013-1844-6

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