Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

Abstract

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

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Acknowledgments

We would like to thank Cristina Silva for providing us the urine samples. This work was supported by Fundação para a Ciência e Tecnologia (Postdoctoral Fellowship SFRH/BPD/74480/2010 and project PIC/IC/82815/2007) and by the European Commission’s Framework Programme (FP7: “COST-Action TD1004: Theragnostics for imaging and therapy”).

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The authors declare that they have no conflict of interest.

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Correspondence to C. Almeida.

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Figure S1
figure3

The 16S rRNA Proteus sequences not detected by theProPNA190 probe. Probe (reverse complementary) alignment with P. vulgaris J01874 (accession number: S000414235); P. mirabilis EF371001 (S000806301); P. mirabilis HQ407312 (S002305608); P. mirabilis HQ231796 (S002409155) and Proteus sp. HQ588328 (S002950968); showing the mismatch base pairs (grey squares) in different the different positions

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Almeida, C., Azevedo, N.F., Bento, J.C. et al. Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH. Eur J Clin Microbiol Infect Dis 32, 781–786 (2013). https://doi.org/10.1007/s10096-012-1808-2

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Keywords

  • Peptide Nucleic Acid
  • Peptide Nucleic Acid Probe
  • Artificial Urine
  • Chromogenic Medium
  • Defibrinated Horse Blood