Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
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We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
KeywordsPeptide Nucleic Acid Peptide Nucleic Acid Probe Artificial Urine Chromogenic Medium Defibrinated Horse Blood
We would like to thank Cristina Silva for providing us the urine samples. This work was supported by Fundação para a Ciência e Tecnologia (Postdoctoral Fellowship SFRH/BPD/74480/2010 and project PIC/IC/82815/2007) and by the European Commission’s Framework Programme (FP7: “COST-Action TD1004: Theragnostics for imaging and therapy”).
Conflict of interest
The authors declare that they have no conflict of interest.
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