Abstract
Molecular epidemiological features of Mycobacterium tuberculosis strains among different patient groups in Russia have not been studied well. The aim of our study was to compare the genotypes of M. tuberculosis strains circulating among tuberculosis (TB) patients from different groups: homeless, human immunodeficiency virus (HIV)-infected, prisoners, and the general population of St. Petersburg citizens. One hundred and forty-two M. tuberculosis complex isolates from different TB patient groups were studied using the spacer oligonucleotide typing (spoligotyping) method. The majority of the studied M. tuberculosis isolates in all groups belonged to the Beijing family (55% among homeless; 77% among HIV-infected; 60% among the general population; 81% among prisoners). There were no significant differences in the Beijing family prevalence among homeless patients, HIV/TB co-infected patients, and the general population of TB patients. The lowest genetic diversity of the pathogen was detected among imprisoned patients. The results of our study demonstrate that M. tuberculosis strains circulating among patients from high-risk groups are also spread among the general population of St. Petersburg citizens.
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Kovalev SY, Kamaev EY, Kravchenko MA, Kurepina NE, Skorniakov SN (2005) Genetic analysis of Mycobacterium tuberculosis strains isolated in Ural region, Russian Federation, by MIRU-VNTR genotyping. Int J Tuberc Lung Dis 9(7):746–752
Surikova OV, Voitech DS, Kuzmicheva G, Tatkov SI, Mokrousov IV, Narvskaya OV et al (2005) Efficient differentiation of Mycobacterium tuberculosis strains of the W-Beijing family from Russia using highly polymorphic VNTR loci. Eur J Epidemiol 20(11):963–974
Mokrousov I, Otten T, Manicheva O, Potapova Y, Vishnevsky B, Narvskaya O et al (2008) Molecular characterization of ofloxacin-resistant Mycobacterium tuberculosis strains from Russia. Antimicrob Agents Chemother 52(8):2937–2939
Mokrousov I, Narvskaya O, Otten T, Vyazovaya A, Limeschenko E, Steklova L et al (2002) Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern Russia. Res Microbiol 153(10):629–637
Kimerling ME, Kluge H, Vezhnina N, Iacovazzi T, Demeulenaere T, Portaels F et al (1999) Inadequacy of the current WHO re-treatment regimen in a central Siberian prison: treatment failure and MDR-TB. Int J Tuberc Lung Dis 3(5):451–453
Toungoussova OS, Mariandyshev A, Bjune G, Sandven P, Caugant DA (2003) Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates in the Archangel prison in Russia: predominance of the W-Beijing clone family. Clin Infect Dis 37(5):665–672
Drobniewski F, Balabanova Y, Nikolayevsky V, Ruddy M, Kuznetzov S, Zakharova S et al (2005) Drug-resistant tuberculosis, clinical virulence, and the dominance of the Beijing strain family in Russia. JAMA 293(22):2726–2731
van Embden JD, Cave MD, Crawford JT, Dale JW, Eisenach KD, Gicquel B et al (1993) Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31(2):406–409
Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S et al (1997) Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35(4):907–914
Brudey K, Driscoll JR, Rigouts L, Prodinger WM, Gori A, Al-Hajoj SA et al (2006) Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol 6:23
TB-Insight: Run SPOTCLUST. Available online at: http://tbinsight.cs.rpi.edu/run_spotclust.html. Accessed 19 May 2011
Toungoussova OS, Sandven P, Mariandyshev AO, Nizovtseva NI, Bjune G, Caugant DA (2002) Spread of drug-resistant Mycobacterium tuberculosis strains of the Beijing genotype in the Archangel Oblast, Russia. J Clin Microbiol 40(6):1930–1937
Norkina OV, Kinsht VN, Mokrousov IV, Kurunov IuN, Krasnov VA, Filipenko ML (2003) The genetic diversity of Mycobacterium tuberculosis and an assessment of risk factors of tuberculosis spread in Russia’s Siberian region by molecular epidemiological methods. Mol Gen Mikrobiol Virusol 3:9–18
Shemyakin IG, Stepanshina VN, Ivanov IY, Lipin MY, Anisimova VA, Onasenko AG et al (2004) Characterization of drug-resistant isolates of Mycobacterium tuberculosis derived from Russian inmates. Int J Tuberc Lung Dis 8(10):1194–1203
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This work was supported by a grant funded by the Federal Science and Innovation Agency of the Russian Federation and the U.S. Civilian Research and Development Foundation (award RUB1-7000-ST-08).
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Chernyaeva, E., Dobrynin, P., Pestova, N. et al. Molecular genetic analysis of Mycobacterium tuberculosis strains spread in different patient groups in St. Petersburg, Russia. Eur J Clin Microbiol Infect Dis 31, 1753–1757 (2012). https://doi.org/10.1007/s10096-011-1497-2
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DOI: https://doi.org/10.1007/s10096-011-1497-2