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In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results

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Abstract

The present study developed an experimental metronidazole-based gel and evaluated its efficacy for the adjuvant treatment of chronic periodontitis. Sixteen patients were randomly allocated into two groups of eight subjects according to the following proposed treatments: (1) scaling and root planing (active control) or (2) scaling and root planing and direct periodontal intrapocket application of 15% metronidazole-based gel in two sites (≥5 mm in depth) (experimental group). Potential changes in the subgingival microbiota were assessed using a DNA Checkerboard method at three proposed times: baseline and following 7 or 30 days of drug administration. High-performance liquid chromatography (HPLC) monitored metronidazole concentrations in the crevicular fluid during treatment. The metronidazole experimental group presented lower bacterial counts than the control group at the three evaluated times (p < 0.01 for baseline, p < 0.001 for 7 or 30 days) when the target species were analyzed as a pool of bacteria. Samples revealed significantly lower counts 7 days after drug administration compared with baseline or after 30 days (p < 0.05). HPLC analysis detected gel 1 h after application. The metronidazole-based gel significantly decreased the total bacterial count at the three evaluated times. Periodontopathogenic species were not different after gel administration.

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Acknowledgments

The authors would like to thank Mr. José Orestes Del Ciampo and Mr. José Roberto Jabor for their technical support. This study was supported by the Foundation for Research Support of São Paulo (FAPESP Process 07/03503-2).

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Authors and Affiliations

  1. Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n° - Monte Alegre, Ribeirão Preto, SP, 14040-904, Brazil

    P. K. Miani, C. do Nascimento, S. Sato, A. V. Filho & V. Pedrazzi

  2. Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    M. J. V. da Fonseca

Authors
  1. P. K. Miani
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  2. C. do Nascimento
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  3. S. Sato
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  4. A. V. Filho
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  5. M. J. V. da Fonseca
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  6. V. Pedrazzi
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Correspondence to V. Pedrazzi.

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Miani, P.K., do Nascimento, C., Sato, S. et al. In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: preliminary results. Eur J Clin Microbiol Infect Dis 31, 1611–1618 (2012). https://doi.org/10.1007/s10096-011-1484-7

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  • DOI: https://doi.org/10.1007/s10096-011-1484-7

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