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Detection of periodontal pathogens in newborns and children with mixed dentition

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Abstract

We report the age-related prevalence of red complex periodontal pathogens, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, along with four strains of orange complex pathogens. The bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (PCR). P. gingivalis was not detected in any site of any subject in the two groups tested. However, T. denticola was not only found in the 6–13 years age group, but also in edentulous newborns at a relatively high prevalence, indicating non-dentition-related colonization by T. denticola. Campylobacter rectus, Prevotella intermedia, T. forsythia, Eikenella corrodens, and Parvimonas micra were found in the oral cavity of most subjects belonging to the 6–13 years age group compared to newborns. This suggested a pronounced association between these colonizing bacteria and the presence of teeth. There was also a strong relation between T. denticola and T. forsythia for their prevalence in the subgingival sulcus of the 6–13 years age group (p < 0.0001), but not in the other sites tested, suggesting that the colonization of dentition-related T. forsythia may be associated with the increased prevalence of non-dentition-related T. denticola in the subgingival sulcus. Overall, these results suggest that dentition is a key determinant of bacterial colonization, especially orange complex bacteria and the red complex bacterium T. forsythia.

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Acknowledgment

The authors are grateful to the São Paulo Foundation for Research (grant, 04/00256-6), the National Council for Scientific and Technological Development (CNPq), the National Institutes of Health (grant, RO1 DE18499), and the Harvard Catalyst Pilot Grant.

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Authors and Affiliations

  1. Department of Periodontology and Preventive Dentistry, Dental Research Division, University of Taubate, 51 Visconde do Rio Branco, Taubaté, SP, 12020-040, Brazil

    J. R. Cortelli

  2. Department of Periodontology, Dental Research Division, University of Taubate, 51 Visconde do Rio Branco, Taubaté, SP, 12020-040, Brazil

    C. B. Fernandes

  3. Department of Periodontology, Dental School, Federal University of Minas Gerais, 6627 Antonio Carlos, Belo Horizonte, MG, 31270-901, Brazil

    F. O. Costa

  4. Nucleus of Periodontolal Research, Dental Research Division, University of Taubate, 51 Visconde do Rio Branco, Taubaté, SP, 12020-040, Brazil

    S. C. Cortelli

  5. Department of Immunology, The Forsyth Institute, 245 First Street, Cambridge, MA, 02142, USA

    M. Kajiya

  6. Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, 02115, USA

    S. C. Howell

  7. Division of Endodontics, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA, 02115, USA

    T. Kawai

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  1. J. R. Cortelli
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  2. C. B. Fernandes
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  3. F. O. Costa
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  4. S. C. Cortelli
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  7. T. Kawai
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Correspondence to J. R. Cortelli.

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Cortelli, J.R., Fernandes, C.B., Costa, F.O. et al. Detection of periodontal pathogens in newborns and children with mixed dentition. Eur J Clin Microbiol Infect Dis 31, 1041–1050 (2012). https://doi.org/10.1007/s10096-011-1405-9

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  • DOI: https://doi.org/10.1007/s10096-011-1405-9

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