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Evaluation of a serum-free transport medium supplemented with cyanobacterial extract, for the optimal survival of Helicobacter pylori from biopsy samples and strains

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Abstract

The purpose of this work was to evaluate the use of an alternative transport medium supplemented with a cyanobacterial extract (CE), free of animal derivatives, to preserve the viability of Helicobacter pylori strains during long-term transportation and allow its recovery from biopsy samples. The transport media evaluated were Mueller–Hinton broth 0.3% agar (MH) and 0.3% of CE (MH-CE). MH broth 5% fetal calf serum (FCS) was used as the reference medium (MH-FCS). Biopsy samples from 134 patients, H. pylori NCTC 11638 and six clinical isolates were studied. A higher recovery (p ≤ 0.001) at 4°C was obtained in MH-CE than in MH-FCS after 96 h of storage. Only MH-CE allowed recovery after 120 h. The H. pylori recovery at room temperature after 96 h was higher (p ≤ 0.005) in MH-CE than in MH-FCS. Similar survival rates were observed in biopsy samples conserved in MH-CE and MH-FCS at 4°C. The recovery after 48 h at room temperature in MH-CE was higher (p ≤ 0.05) than MH-FCS and was the only medium allowing recovery after 72 h. The MH-CE medium is a simple, inexpensive and animal derivatives-free transport medium that can be used to preserve H. pylori viability and its recovery from biopsy samples.

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Acknowledgements

We thank Patricia Gomez and Ruben Majul for providing the gastric biopsy specimens. This work was supported by funds of C y T project 9303 from the Universidad Nacional de San Luis, San Luis, Argentina.

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Authors and Affiliations

  1. Área Microbiología, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis (UNSL), Bloque I, Primer Piso, Avda. Ejército de los Andes 950, 5700, San Luis, Argentina

    A. E. Vega, H. J. Silva & T. I. Cortiñas

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  1. A. E. Vega
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  2. H. J. Silva
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  3. T. I. Cortiñas
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Correspondence to T. I. Cortiñas.

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Vega, A.E., Silva, H.J. & Cortiñas, T.I. Evaluation of a serum-free transport medium supplemented with cyanobacterial extract, for the optimal survival of Helicobacter pylori from biopsy samples and strains. Eur J Clin Microbiol Infect Dis 31, 135–139 (2012). https://doi.org/10.1007/s10096-011-1285-z

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  • DOI: https://doi.org/10.1007/s10096-011-1285-z

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