Abstract
In the last several years, West Nile virus (WNV) was proven to be present especially in the neighboring countries of Austria, such as Italy, Hungary, and the Czech Republic, as well as in eastern parts of Austria, where it was detected in migratory and domestic birds. In summer 2010, infections with WNV were reported from Romania and northern Greece with about 150 diseased and increasingly fatal cases. We tested the sera of 1,607 blood donors from North Tyrol (Austria) and South Tyrol (Italy) for antibodies against WNV by using IgG enzyme-linked immunosorbent assay (ELISA). Initial results of the ELISA tests showed seroprevalence rates of 46.2% in North Tyrol and 0.5% in South Tyrol, which turned out to be false-positive cross-reactions with antibodies against tick-borne encephalitis virus (TBEV) by adjacent neutralization assays. These results indicate that seropositivity against WNV requires confirmation by neutralization assays, as cross-reactivity with TBEV is frequent and because, currently, WNV is not endemic in the study area.
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References
Reiter P (2010) West Nile virus in Europe: understanding the present to gauge the future. Euro Surveill 15(10):pii=19508
Calistri P, Giovannini A, Hubálek Z, Ionescu A, Monaco F, Savini G et al (2010) Epidemiology of West Nile in Europe and in the Mediterranean basin. Open Virol J 4:29–37
Zeller HG, Schuffenecker I (2004) West Nile virus: an overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis 23:147–156
Papa A, Danis K, Baka A, Bakas A, Dougas G, Lytras T et al (2010) Ongoing outbreak of West Nile virus infections in humans in Greece, July–August 2010. Euro Surveill 15(34):pii=19644
Bakonyi T, Ivanics E, Erdélyi K, Ursu K, Ferenczi E, Weissenböck H et al (2006) Lineage 1 and 2 strains of encephalitic West Nile virus, central Europe. Emerg Infect Dis 12(4):618–623
Wodak E, Richter S, Bagó Z, Revilla-Fernández S, Weissenböck H, Nowotny N et al (2011) Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009. Vet Microbiol 149:358–366
Bakonyi T, Hubálek Z, Rudolf I, Nowotny N (2005) Novel flavivirus or new lineage of West Nile virus, central Europe. Emerg Infect Dis 11(2):225–231
Niedrig M, Sonnenberg K, Steinhagen K, Paweska JT (2007) Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation. J Virol Methods 139(1):103–105
Roth D, Henry B, Mak S, Fraser M, Taylor M, Li M et al (2010) West Nile virus range expansion into British Columbia. Emerg Infect Dis 16(8):1251–1258
Turell MJ, O’Guinn M, Oliver J (2000) Potential for New York mosquitoes to transmit West Nile virus. Am J Trop Med Hyg 62(3):413–414
Gibbs SEJ, Wimberly MC, Madden M, Masour J, Yabsley MJ, Stallknecht DE (2006) Factors affecting the geographic distribution of West Nile virus in Georgia, USA: 2002–2004. Vector Borne Zoonotic Dis 6:73–82
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This work was supported by the European Union, the Republic of Austria, the Republic of Italy, the State of Tyrol, and the Autonomous Province of Bozen (Interreg).
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Sonnleitner, S.T., Simeoni, J., Schmutzhard, E. et al. Absence of indigenous specific West Nile virus antibodies in Tyrolean blood donors. Eur J Clin Microbiol Infect Dis 31, 77–81 (2012). https://doi.org/10.1007/s10096-011-1279-x
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DOI: https://doi.org/10.1007/s10096-011-1279-x