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Soluble triggering receptor expressed on myeloid cells in sputum of patients with community-acquired pneumonia or pulmonary tuberculosis: a pilot study

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Abstract

Soluble triggering receptor expressed on myeloid cells (s-TREM-1) is upregulated on the surface of inflammatory cells in the presence of bacterial infections, apparently excluding those due to Mycobacterium tuberculosis. Therefore, sputum concentrations of s-TREM-1 may be of value in distinguishing bacterial pneumonia from pulmonary tuberculosis (PTB) in patients with respiratory infections. The current pilot study was designed to evaluate whether s-TREM-1 concentrations measured in the sputum of patients with suspected community-acquired pneumonia (CAP) allowed differentiation of those patients with PTB from other causes of pneumonia and to correlate s-TREM-1 with CURB-65, a marker of disease severity. Soluble s-TREM-1 concentrations were measured in sputum samples from patients admitted to a tertiary hospital with CAP or PTB by means of an ELISA procedure. Soluble-TREM-1 was readily detectable and quantifiable in sputum samples from patients with both CAP and PTB, with concentrations of 234 ± 47 and 178 ± 36 pg/ml respectively, but did not differ significantly between the two groups. However, patients with PTB had significantly lower leukocyte counts, 9 ± 1.3 vs 15 ± 1.4 × 109/l compared with those without PTB. Interestingly, sputum s-TREM-1 concentrations correlated significantly with the CURB-65 pneumonia severity score calculated at the time of admission. Soluble-TREM-1 expression is upregulated in patients with both CAP and PTB, but does not differentiate between these two conditions. Sputum concentrations of s-TREM-1 may predict the severity of disease in patients with CAP.

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The authors declare no conflict of interests.

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Authors and Affiliations

  1. Department of Internal Medicine, Faculty of Health Sciences, University of Pretoria, PO Box 2034, Pretoria, 0001, South Africa

    G. R. Tintinger, J. J. van der Merwe & P. Rheeder

  2. Department of Immunology, Medical Research Council Unit for Inflammation and Immunity, University of Pretoria and Tshwane Academic Division of the National Health Laboratory Service, Pretoria, South Africa

    H. Fickl & R. Anderson

  3. Division of Pulmonology, Department of Internal Medicine, Charlotte Maxeke Johannesburg Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa

    C. Feldman

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  1. G. R. Tintinger
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  2. J. J. van der Merwe
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  3. H. Fickl
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  4. P. Rheeder
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  5. C. Feldman
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  6. R. Anderson
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Correspondence to G. R. Tintinger.

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Tintinger, G.R., van der Merwe, J.J., Fickl, H. et al. Soluble triggering receptor expressed on myeloid cells in sputum of patients with community-acquired pneumonia or pulmonary tuberculosis: a pilot study. Eur J Clin Microbiol Infect Dis 31, 73–76 (2012). https://doi.org/10.1007/s10096-011-1278-y

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  • DOI: https://doi.org/10.1007/s10096-011-1278-y

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