Abstract
Plasmid-mediated class C β-lactamases are reported from Escherichia coli and Klebsiella pneumoniae with increasing frequency. No screening and confirmatory tests have been uniformly established for these strains. We investigated for the presence of plasmid-mediated AmpC production in 51 clinical isolates of Enterobacteriaceae, comparing two different boronic acid formulations, phenylboronic acid (PB) and 3-(N-Boc-amino)phenylboronic acid (APB), using polymerase chain reaction (PCR). PB performed better than APB.
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Acknowledgements
We would like to thank Justin Ellem (Institute of Clinical Pathology and Medical Research, Westmead Hospital, Sydney) and Kate McCarthy (Sullivan Nicolaides Pathology, Brisbane) for providing some of the isolates tested, and Lee Thomas (Institute of Clinical Pathology and Medical Research, Westmead Hospital, Sydney) and Jeanette Pham (SEALS Pathology, Sydney) for providing the positive control strains.
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Su, WY., Gottlieb, T. & Merlino, J. Optimal phenotypic testing of AmpC beta-lactamases using boronic acid solutions. Eur J Clin Microbiol Infect Dis 31, 49–51 (2012). https://doi.org/10.1007/s10096-011-1271-5
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DOI: https://doi.org/10.1007/s10096-011-1271-5