Abstract
The screening for and diagnosis of bacteriological infections often involves the collection and transportation of swab samples. The Copan ESwab was compared with the dry cotton Copan swab for methicillin-resistant Staphylococcus aureus (MRSA) screening (n = 200 paired samples) and with the Amies agar gel swab (Copan) for the sampling of burn and orthopaedic wounds (n = 203 paired samples) in terms of Gram staining and bacterial recovery. Gram stains performed with ESwab liquid showed significantly more Gram-negative rods, streptococci, Gram-positive cocci, Gram-positive rods, polymorphonuclear cells, lymphocytes and red blood cells than Gram stains from dry swabs. Bacterial recovery was significantly higher with ESwab (p < 0.01, for both MRSA screening and wounds, quantitative/semi-quantitative method). This lead to a slightly higher detection rate of MRSA (128 vs. 124 MRSA-positive ESwabs and dry swabs, respectively, p = 0.50) and a higher detection rate of coagulase-negative Staphylococcus spp. (44 isolates with ESwab vs. 29 with Amies gel swab, p = 0.001) and Enterococcus spp. (15 isolates with ESwab vs. 7 isolates with Amies gel swab, p = 0.005) with ESwab (quantitative method). We confirmed that ESwab has a high performance for Gram stains and a higher bacterial recovery than dry and Amies gel swabs when using clinical samples for MRSA screening and wound sampling.
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References
Van Horn KG, Audette CD, Sebeck D, Tucker KA (2008) Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability. J Clin Microbiol 46(5):1655–1658
Osterblad M, Järvinen H, Lönnqvist K, Huikko S, Laippala P, Viljanto J, Arvilommi H, Huovinen P (2003) Evaluation of a new cellulose sponge-tipped swab for microbiological sampling: a laboratory and clinical investigation. J Clin Microbiol 41:1894–1900
Copan Innovation (2008) ESwab collection and transport system. Product insert and how to use guide. Copan Innovation, Brescia, Italy
Nys S, Vijgen S, Magerman K, Cartuyvels R (2010) Comparison of Copan eSwab with the Copan Venturi Transystem for the quantitative survival of Escherichia coli, Streptococcus agalactiae and Candida albicans. Eur J Clin Microbiol Infect Dis 29:453–456
Van Horn KG, Audette CD, Tucker KA, Sebeck D (2008) Comparison of 3 swab transport systems for direct release and recovery of aerobic and anaerobic bacteria. Diagn Microbiol Infect Dis 62:471–473
Body B, Arbique J, Bourbeau P et al (2003) Quality Control of Microbiological Transport Systems; Clinical Laboratory and Standards Institute (CLSI) Approved Standard M40-A. Pennsylvania, PA, USA. Dec. report no.: M40-A, volume 23, number 34
Fontana C, Favaro M, Limongi D, Pivonkova J, Favalli C (2009) Comparison of the eSwab collection and transportation system to an amies gel transystem for Gram stain of clinical specimens. BMC Res Notes 2:244
Daley P, Castriciano S, Chernesky M, Smieja M (2006) Comparison of flocked and rayon swabs for collection of respiratory epithelial cells from uninfected volunteers and symptomatic patients. J Clin Microbiol 44:2265–2267
Loens K, Van Heirstraeten L, Malhotra-Kumar S, Goossens H, Ieven M (2009) Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections. J Clin Microbiol 47(1):21–31
Smismans A, Verhaegen J, Schuermans A, Frans J (2009) Evaluation of the Copan ESwab transport system for the detection of methicillin-resistant Staphylococcus aureus: a laboratory and clinical study. Diagn Microbiol Infect Dis 65(2):108–111
Human RP, Jones GA (2004) Evaluation of swab transport systems against a published standard. J Clin Pathol 57:762–763
Human RP, Jones GA (2006) A new concept for transporting clinical material on flocked swabs in liquid Amies medium. In: Proceedings of the 106th General Meeting of the American Society for Microbiology (ASM), Orlando, FL, USA, 21–25 May 2006
Bourbeau PP, Swartz BL (2009) First evaluation of the WASP, a new automated microbiology plating instrument. J Clin Microbiol 47(4):1101–1106
Acknowledgements
We sincerely thank Rudi Deleus (Intensive Care Unit Burn Wounds), Godelieve Nicaise, Raf Beneens and Bart Vander Elst (Geriatric Medicine), Marleen Van Eygen (General Internal Medicine) and Jan Bosque (Septic Orthopaedics) for their readiness to procure all of the clinical samples, and Jos Vandeven (Laboratory Medicine) for the help in the identification of the micro-organisms.
Both the ESwab MRSA Collection Kits and the conventional ESwabs were kindly provided by Copan.
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Saegeman, V., Flamaing, J., Muller, J. et al. Clinical evaluation of the Copan ESwab for methicillin-resistant Staphylococcus aureus detection and culture of wounds. Eur J Clin Microbiol Infect Dis 30, 943–949 (2011). https://doi.org/10.1007/s10096-011-1178-1
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DOI: https://doi.org/10.1007/s10096-011-1178-1