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Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods

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Abstract

The VITEK 2 AST-N111 card was evaluated for detection of extended-spectrum beta-lactamases (ESBLs) by testing 51 ESBL positive and 50 ESBL negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by PCR and sequencing. The advanced expert system (AES) of the VITEK 2 system achieved sensitivity and specificity values of 100% and 96.0%, respectively. The ESBL test of the VITEK 2 AST-N111 card showed a sensitivity of 92.1% and a specificity of 90.0%. Contradictory results obtained with the two VITEK 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of AES and ESBL tests of the AST-N111 card in association with combination disk tests in case of contradictory results seems to be a reliable method for ESBL detection.

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Acknowledgements

Neil Woodford and Jesus Mingorance are acknowledged for donation of the control strains. We are grateful to Gabi Heinze and Simone Pusch for expert technical assistance.

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Authors and Affiliations

  1. Institute of Hygiene and Microbiology, University of Wuerzburg, Josef-Schneider-Str. 2 / E1, 97080, Wuerzburg, Germany

    G. Valenza, S. Müller, C. Schmitt, D. Turnwald, T-T. Lam, M. Frosch & M. Abele-Horn

  2. Robert Koch Institute, Wernigerode, Germany

    Y. Pfeifer

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  1. G. Valenza
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  2. S. Müller
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  3. C. Schmitt
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  4. D. Turnwald
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  5. T-T. Lam
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  6. M. Frosch
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  7. M. Abele-Horn
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  8. Y. Pfeifer
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Correspondence to G. Valenza.

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Valenza, G., Müller, S., Schmitt, C. et al. Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods. Eur J Clin Microbiol Infect Dis 30, 869–872 (2011). https://doi.org/10.1007/s10096-011-1169-2

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  • DOI: https://doi.org/10.1007/s10096-011-1169-2

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