Abstract
Rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. We used a semi-automated repetitive sequence-based polymerase chain reaction (Rep-PCR) typing assay and pulsed field gel electrophoresis (PFGE) to investigate the relationship between local Klebsiella pneumoniae (K. pneumoniae) producing extended spectrum β-lactamases (ESBLs) and their relation to recognized Danish outbreak strains. PFGE and Rep-PCR produced similar clustering among isolates. Individual isolates from each cluster were further characterized by PCR amplification and sequencing of bla TEM, bla SHV, and bla CTX-M, and multilocus sequence typing (MLST). Thirty-five out of 52 ESBL-producing K. pneumoniae isolates were ST15 and bla CTX-M15, bla SHV-28, and bla TEM-1 positive by PCR. Ten out of 52 were ST16 and tested positive for bla CTX-M15, bla SHV-1, and bla TEM-1. Isolates from previously recognized hospital outbreaks were also ST15 and PCR positive for bla CTX-M15, bla SHV-28, and bla TEM-1, and typed within the main cluster by both Rep-PCR and PFGE. In conclusion, K. pneumoniae ST15 containing bla CTX-M15 and bla SHV-28 constitutes an epidemic clone in the Copenhagen area and this clone can be rapidly recognized by semi-automated Rep-PCR.
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The authors gratefully acknowledge support from bioMérieux for providing access to the Agilent 2100 Bioanalyzer.
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This work was supported by Fondation Idella (grants 3.3.5.-2007 and 3.3.5/2008/II to K.S.). All authors declare that they do not have any commercial interests or associations that pose a conflict of interest with regard to this article.
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Nielsen, J.B., Skov, M.N., Jørgensen, R.L. et al. Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay. Eur J Clin Microbiol Infect Dis 30, 773–778 (2011). https://doi.org/10.1007/s10096-011-1153-x
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DOI: https://doi.org/10.1007/s10096-011-1153-x