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Microbial diversity of supra- and subgingival biofilms on freshly colonized titanium implant abutments in the human mouth

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Abstract

Supra- and subgingival biofilm formation is considered to be mainly responsible for early implant failure caused by inflammations of periimplant tissues. Nevertheless, little is known about the complex microbial diversity and interindividual similarities around dental implants. An atraumatic assessment was made of the diversity of microbial communities around titanium implants by single strand conformation polymorphism (SSCP) analysis of the 16S rRNA gene amplicons as well as subsequent sequence analysis. Samples of adherent supra- and subgingival periimplant biofilms were collected from ten patients. Additionally, samples of sulcusfluid were taken at titanium implant abutments and remaining teeth. The bacteria in the samples were characterized by SSCP and sequence analysis. A high diversity of bacteria varying between patients and within one patient at different locations was found. Bacteria characteristic for sulcusfluid and supra- and subgingival biofilm communities were identified. Sulcusfluid of the abutments showed higher abundance of Streptococcus species than from residual teeth. Prevotella and Rothia species frequently reported from the oral cavity were not detected at the abutments suggesting a role as late colonizers. Different niches in the human mouth are characterized by specific groups of bacteria. Implant abutments are a very valuable approach to study dental biofilm development in vivo.

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Acknowledgements

We are indebted to Jennifer Knaak for her excellent technical assistance. This work was supported by a grant from the German Federal Ministry for Science, Education and Research (Project No. 01 KI 07 96) and by the German Research Foundation (DFG-SFB Collaborative Research Center 599).

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Authors and Affiliations

  1. Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany

    W. Heuer & M. Stiesch

  2. Chemical Microbiology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Braunschweig, Germany

    W. R. Abraham

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  1. W. Heuer
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  2. M. Stiesch
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  3. W. R. Abraham
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Correspondence to W. R. Abraham.

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Heuer, W., Stiesch, M. & Abraham, W.R. Microbial diversity of supra- and subgingival biofilms on freshly colonized titanium implant abutments in the human mouth. Eur J Clin Microbiol Infect Dis 30, 193–200 (2011). https://doi.org/10.1007/s10096-010-1068-y

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  • DOI: https://doi.org/10.1007/s10096-010-1068-y

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