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The effect of manuka honey on the structure of Pseudomonas aeruginosa

European Journal of Clinical Microbiology & Infectious Diseases volume 30, pages 167–171 (2011)Cite this article

Abstract

The purpose of this study was to investigate the effects of manuka honey on the structural integrity of Pseudomonas aeruginosa ATCC 27853. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of manuka honey for P. aeruginosa were determined by a microtitre plate method, and the survival of bacteria exposed to a bactericidal concentration of manuka honey was monitored. The effect of manuka honey on the structure of the bacteria was investigated using scanning and transmission electron microscopy (SEM and TEM, respectively). The MIC and MBC values of manuka honey against P. aeruginosa were 9.5% (w/v) and 12% (w/v) respectively; a time–kill curve demonstrated a bactericidal rather than a bacteriostatic effect, with a 5 log reduction estimated within 257 min. Using SEM, loss of structural integrity and marked changes in cell shape and surface were observed in honey-treated cultures. With TEM, these changes were confirmed, and evidence of extensive cell disruption and lysis was found. Manuka honey does not induce the same structural changes in P. aeruginosa as those observed in staphylococci. Our results indicate that manuka honey has the potential to be an effective inhibitor of P. aeruginosa.

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References

  1. Branski LK, Al-Mousawi A, Rivero H, Jeschke MG, Sanford AP, Herndon DN (2009) Emerging infections in burns. Surg Infect 10(5):389–397

    Article  Google Scholar 

  2. Keen EF 3rd, Robinson BJ, Hospenthal DR, Aldous WK, Wolf SE, Chung KK, Murray CK (2010) Prevalence of multidrug-resistant organisms recovered at a military burn center. Burns 36(6):819–825

    Article  PubMed  Google Scholar 

  3. Fazli M, Bjarnsholt T, Kirketerp-Møller K, Jørgensen B, Andersen AS, Krogfelt KA, Givskov M, Tolker-Nielsen T (2009) Nonrandom distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds. J Clin Microbiol 47(12):4084–4089

    Article  PubMed  Google Scholar 

  4. Blair SE, Carter DA (2005) The potential for honey in the management of wound and infection. J Australian Infection Control 10:24–31

    Google Scholar 

  5. Molan PC (1992) The antibacterial activity of honey. 1. The nature of the antibacterial activity. Bee World 73:5–28

    Google Scholar 

  6. Cooper RA, Halas E, Molan PC (2002) The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. J Burn Care Rehabil 23:366–370

    CAS  Article  PubMed  Google Scholar 

  7. Cooper RA, Molan PC, Harding KG (2002) The sensitivity to honey of Gram-positive cocci of clinical significance isolated from wounds. J Appl Microbiol 93:857–863

    CAS  Article  PubMed  Google Scholar 

  8. French VM, Cooper RA, Molan PC (2005) The antibacterial activity of honey against coagulase-negative staphylococci. J Antimicrob Chemother 56:228–231

    CAS  Article  PubMed  Google Scholar 

  9. Wahdan HAL (1998) Causes of the antimicrobial activity of honey. Infection 26:26–31

    CAS  Article  PubMed  Google Scholar 

  10. Willix DJ, Molan PC, Harfoot CG (1992) A comparison of the sensitivity of wound-infecting species of bacteria to the antibacterial activity of manuka honey and other honey. J Appl Bacteriol 73:388–394

    CAS  PubMed  Google Scholar 

  11. Karayil S, Deshpande SD, Koppikar GV (1998) Effect of honey on multidrug resistant organisms and its synergistic action with three common antibiotics. J Postgrad Med 44:93–96

    CAS  PubMed  Google Scholar 

  12. Kwakman PH, te Velde AA, de Boer L, Speijer D, Vandenbroucke-Grauls CM, Zaat SA (2010) How honeys kills bacteria. FASEB J 24(7):2576–2582

    CAS  Article  PubMed  Google Scholar 

  13. Blair SE, Cokcetin NN, Harry EJ, Carter DA (2009) The unusual antibacterial activity of medical-grade Leptospermum honey: antibacterial spectrum, resistance and transcriptome analysis. Eur J Clin Microbiol Infect Dis 28(10):1199–1208

    CAS  Article  PubMed  Google Scholar 

  14. Henriques AF, Jenkins RE, Burton NF, Cooper RA (2009) The intracellular effects of manuka honey on Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 29:45–50. doi:10.1007/s10096-009-0817-2

    Article  PubMed  Google Scholar 

  15. Allen KL, Molan PC, Reid GM (1991) A survey of the antibacterial activity of some New Zealand honeys. J Pharm Pharmacol 43(12):817–882

    CAS  PubMed  Google Scholar 

  16. Lemar KM, Turner MP, Lloyd D (2002) Garlic (Allium sativum) as an anti-Candida agent: a comparison of the efficacy of fresh garlic and freeze-dried extracts. J Appl Microbiol 93:398–405

    CAS  Article  PubMed  Google Scholar 

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Acknowledgements

We wish to thank Drs. Hann and Turner of the Electron Microscope Unit at Cardiff University, Wales, UK.

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Author notes

  1. A. F. Henriques

    Present address: Instituto de Biologia Molecular e Celular, Group of Molecular Microbiology, Universidade do Porto, Rua do Campo Alegre, 823, Porto, Portugal

Authors and Affiliations

  1. Centre for Biomedical Sciences, Cardiff School of Health Sciences, University of Wales Institute Cardiff, Western Avenue, Cardiff, CF5 2YB, UK

    A. F. Henriques, R. E. Jenkins, N. F. Burton & R. A. Cooper

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  1. A. F. Henriques
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  2. R. E. Jenkins
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  3. N. F. Burton
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Correspondence to R. A. Cooper.

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Henriques, A.F., Jenkins, R.E., Burton, N.F. et al. The effect of manuka honey on the structure of Pseudomonas aeruginosa . Eur J Clin Microbiol Infect Dis 30, 167–171 (2011). https://doi.org/10.1007/s10096-010-1065-1

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  • DOI: https://doi.org/10.1007/s10096-010-1065-1

Keywords

  • Minimum Inhibitory Concentration
  • Minimum Bactericidal Concentration
  • Display Cell Surface
  • Manuka Honey
  • Kill Curve